| 摘要 | 第8-10页 |
| Abstract | 第10-12页 |
| 1 Introduction | 第13-16页 |
| 1.1 Background of Study | 第13-14页 |
| 1.2 Objectives of this Research | 第14-15页 |
| 1.3 Research Grants | 第15-16页 |
| 2 Literature Review | 第16-33页 |
| 2.1 The Concept of Probiotics | 第16-17页 |
| 2.2 Streptococcus thermophilus | 第17-18页 |
| 2.3 Probiotic Lactic Acid Bacteria Use in Yoghurt Starter Cultures | 第18-19页 |
| 2.4 Probiotic Lactic Acid Bacteria and Obesity Treatment | 第19-20页 |
| 2.5 Probiotic Lactic Acid Bacteria and Type-2 Diabetes Treatment | 第20-22页 |
| 2.6 Probiotic Lactic acid bacteria as starter cultures in ice cream | 第22-23页 |
| 2.7 Some Current Challenges with the use of Lactic acid bacteria as Probiotics | 第23-24页 |
| 2.8 Lactic Acid Bacteria Inhibition of Foodborne Pathogens | 第24-31页 |
| 2.9 Bacterial Vaginosis suppression by Lactic Acid Bacteria | 第31-33页 |
| 3 Materials and Methods | 第33-38页 |
| 3.1 Materials | 第33页 |
| 3.1.1 Bacteria strains and Growth Conditions | 第33页 |
| 3.1.2 Experimental Animals | 第33页 |
| 3.2 Methodology | 第33-36页 |
| 3.2.1 Bioinformatics of S. thermophilus KLDS 3.1003 | 第33-34页 |
| 3.2.2 Antimicrobial screening of single-culture bacteria and CFS | 第34页 |
| 3.2.3 Effect of p H and other treatments on the antimicrobial activity of CFS | 第34页 |
| 3.2.4 Tolerance assays | 第34-35页 |
| 3.2.5 Effect of carbon sources on the lactic and acetic acid production of bacterialstrains | 第35页 |
| 3.2.6 Animal Toxicity Studies | 第35-36页 |
| 3.3 Statistical Analyses | 第36-38页 |
| 4 Results | 第38-61页 |
| 4.1 Bioinformatics of S. thermophilus KLDS 3.1003 | 第38-45页 |
| 4.1.1 Phylogenetic Analyses | 第38页 |
| 4.1.2 Genome Characteristics of S. thermophilus KLDS 3.1003 | 第38页 |
| 4.1.3 Comparatove Genome Report of Some Sequenced S. thermophilus strains | 第38-39页 |
| 4.1.4 Amino acid-coding genes of S. thermophilus KLDS 3.1003 | 第39页 |
| 4.1.5 CRISPR-cas Properties of S. thermophilus KLDS 3.1003 | 第39页 |
| 4.1.6 EPS Gene Cluster of S. thermophilus KLDS 3.1003 | 第39-42页 |
| 4.1.7 Gene-coding Enzymes for EPS Biosynthesis | 第42-45页 |
| 4.2 In vitro studies | 第45-52页 |
| 4.2.1 Inhibition zone determination of bacterial cells and CFS | 第45-47页 |
| 4.2.2 Antimicrobial Substance Screening Results | 第47-48页 |
| 4.2.3 Bile and Acid Tolerance Assays | 第48-49页 |
| 4.2.4 Lactic and Acetic Acid Production Profiles of Probiotic LABs | 第49-52页 |
| 4.3 In vivo studies | 第52-61页 |
| 4.3.1 Weight of Study Animals | 第52页 |
| 4.3.2 Blood biochemical analyses | 第52-53页 |
| 4.3.3 Haematological analyses | 第53-54页 |
| 4.3.4 Histopathology and Necropsy Analyses | 第54-61页 |
| 5 Discussion | 第61-71页 |
| 5.1 Bioinformatics of S. thermophilus KLDS 3.1003 | 第61-62页 |
| 5.1.1 Phylogenetic analyses and CRISPR cas properties | 第61页 |
| 5.1.2 EPS gene cluster of S. thermophilus KLDS 3.1003 | 第61-62页 |
| 5.1.3 Gene-coding enzymes for carbohydrate fermentation | 第62页 |
| 5.2 Results from in vitro studies | 第62-66页 |
| 5.2.1 Inhibition zone determination of bacterial cells and their CFS | 第62-64页 |
| 5.2.2 Effects of various treatments on CFS antimicrobial activities | 第64页 |
| 5.2.3 Tolerance assays of LAB strains in gastric juice, intestinal juice and bile salts | 第64-65页 |
| 5.2.4 Supplementary carbon sources on lactic and acetic acid production | 第65-66页 |
| 5.3 Results from in vivo studies | 第66-71页 |
| 5.3.1 Effects of probiotic treatments on weights of experimental animals | 第66-67页 |
| 5.3.2 Effects of probiotic administration on blood biochemical parameters | 第67-68页 |
| 5.3.3 Effects of probiotic administration on haematological parameters | 第68-69页 |
| 5.3.4 Effects of probiotic administration on histopathological analyses | 第69-71页 |
| 6 Conclusions | 第71-72页 |
| 7 Future Considerations | 第72-74页 |
| 8 Innovations of this Research | 第74-75页 |
| 9 Achievements | 第75-76页 |
| 10 Nucleotide Sequence Accession Number | 第76-77页 |
| Acknowledgements | 第77-78页 |
| References | 第78-98页 |
| Appendix | 第98-157页 |
| Papers published in the period of Ph.D Education | 第157-158页 |
