| 摘要 | 第10-12页 |
| Abstract | 第12-13页 |
| 1 Introduction | 第14-20页 |
| 2 Review of literature | 第20-36页 |
| 2.1 Probiotics | 第20-21页 |
| 2.1.1 Description | 第20页 |
| 2.1.2 The contrivance of probiotics accentuation | 第20页 |
| 2.1.3 Examples of Immune and Gut benefits of probiotics | 第20-21页 |
| 2.1.4 Synergistic effects | 第21页 |
| 2.1.5 Barriers in utilization of probiotics | 第21页 |
| 2.2 Prebiotics | 第21-22页 |
| 2.2.1 Description | 第21页 |
| 2.2.2 Health vantages | 第21-22页 |
| 2.3 Encapsulation | 第22页 |
| 2.4 Utilization of microencapsulated probiotics in foodstuffs | 第22-24页 |
| 2.4.1 Cheese | 第22-23页 |
| 2.4.2 Yogurt | 第23页 |
| 2.4.3 Frozen dairy dessert | 第23-24页 |
| 2.5 Potential future advances encapsulation of probiotics | 第24页 |
| 2.6 Spray-drying | 第24-25页 |
| 2.7 Characteristics of wall materials for microencapsulation | 第25-27页 |
| 2.7.1 Assortment of wall materials | 第25页 |
| 2.7.2 Most frequently and accustomed wall materials | 第25-27页 |
| 2.8 Bacteriocins | 第27页 |
| 2.8.1 Cataloging of bacteriocins | 第27页 |
| 2.9 Lactobacillus plantarum | 第27-29页 |
| 2.9.1 Bacteriocins from L. Plantarum extracted from milk based products | 第27-28页 |
| 2.9.2 Uses of L. plantarum and its bacteriocins | 第28-29页 |
| 2.10 Role of probiotics for heavy metals | 第29页 |
| 2.11 Gut microflora | 第29-30页 |
| 2.12 Evasive functions | 第30页 |
| 2.13 Probiotics and prebiotics functionality | 第30-31页 |
| 2.14 Lead and its absorption | 第31-36页 |
| 2.14.1 Sources | 第31-32页 |
| 2.14.2 Haematotoxicity | 第32-33页 |
| 2.14.3 Neurotoxicity | 第33页 |
| 2.14.4 Renal toxicity | 第33页 |
| 2.14.5 Cardiovascular toxicity | 第33-34页 |
| 2.14.6 Gastrointestinal toxicity | 第34页 |
| 2.14.7 Hepatotoxicity | 第34页 |
| 2.14.8 Procreative and progressive noxiousness | 第34-35页 |
| 2.14.9 Genotoxicity | 第35页 |
| 2.14.10 Carcinogenicity | 第35-36页 |
| 3 Material components and methods | 第36-49页 |
| 3.1 Experimental material | 第36页 |
| 3.2 Cell Free Supernatants preparation (CFS) | 第36页 |
| 3.3 Screening of L. plantarum antimicrobial potentiality | 第36-37页 |
| 3.4 Antibacterial ambit of L. plantarum KLDS 1.0344 | 第37页 |
| 3.5 Antimicrobial substances rectification of L. plantarum KLDS 1.0344 by enzymes | 第37页 |
| 3.6 Determination of acid production of L. plantanan KLDS 1.0344 | 第37-38页 |
| 3.7 Mixed culture inhibition assay | 第38页 |
| 3.8 Bacteriocin production | 第38页 |
| 3.9 Bacteriocin fractional refinement by dialysis and ammonium sulphate precipitation | 第38-39页 |
| 3.10 Thermal stability of partially purified bacteriocin | 第39页 |
| 3.11 pH stability of partially purified bacteriocin | 第39页 |
| 3.12 Cell culture preparation | 第39页 |
| 3.13 Preparation of drying carrier media | 第39-40页 |
| 3.14 Spray drying and storage | 第40页 |
| 3.15 Survival of microencapsulated Z. pAjwtorwwz KLDS 1.0344 during storage | 第40-41页 |
| 3.16 Survivability during in vitro digestion | 第41页 |
| 3.17 Characterization of spray dried powders | 第41-43页 |
| 3.17.1 Moisture content | 第41页 |
| 3.17.2 Water activity | 第41-42页 |
| 3.17.3 Hygroscopicity | 第42页 |
| 3.17.4 Differential Scanning Calorimetry (DSC) Analyses | 第42页 |
| 3.17.5 Particle size | 第42页 |
| 3.17.6 Scanning electron microscopy | 第42-43页 |
| 3.18 Bacterial strain and culture | 第43-44页 |
| 3.19 Animals and experimental design | 第44页 |
| 3.20 Determination of chemical elements in tissues and feces | 第44页 |
| 3.21 Determination of lead in blood, tissues and feces | 第44页 |
| 3.22 Determination of SOD activity in tissues (kidney and liver) | 第44-45页 |
| 3.23 Determination of MDA activity in tissues (kidney and liver) | 第45-46页 |
| 3.24 Determination of GSH activity in tissues (kidney and liver) | 第46页 |
| 3.25 Determination of GH-PX activity in tissues (kidney and liver) | 第46-48页 |
| 3.26 Determination of CAT activity in tissues (kidney and liver) | 第48页 |
| 3.27 Histopathological studies | 第48页 |
| 3.28 Statistical analyses | 第48-49页 |
| 4 Results and Discussion | 第49-92页 |
| 4.1 Antimicrobial potentiality screening by Oxford cup technique | 第49-51页 |
| 4.2 Determination of inhibitory substances of L. plantanan KLDS 1.0344 and evaluate the effect ofproteases enzymes on their activity | 第51-53页 |
| 4.3 Partial purification, thermal and pH stability of bacteriocin | 第53-56页 |
| 4.4 Mixed culture inhibition assay | 第56-62页 |
| 4.5 Impact of polysaccharides on storage stability of i. KLDS 1.0344 in spray-driedpowders at 25℃ | 第62页 |
| 4.6 Survival of microencapsulated bacteria in GI simulated conditions | 第62-66页 |
| 4.7 Residual moisture content (MC), Hygroscopicity (H) and Water activity (a_w) | 第66-69页 |
| 4.8 Thermophysical properties of spray dried powder | 第69-72页 |
| 4.9 Particle size distribution and morphological properties | 第72-73页 |
| 4.10 Body weight of mice | 第73-78页 |
| 4.11 Pb contents in the excreta, liver, kidneys and blood | 第78-82页 |
| 4.12 Ca,Zn,Fe,and Mg quantities in the kidneys and liver | 第82-83页 |
| 4.13 MDA, GSH, SOD, GPx, and CAT in liver and kidneys | 第83-84页 |
| 4.14 Histopathological appraisal | 第84-92页 |
| 5 Summary | 第92-94页 |
| Acknowledgements | 第94-95页 |
| References | 第95-116页 |
| Papers published in the period of Ph.D. education | 第116页 |
