| 中文摘要 | 第4-5页 |
| Abstract | 第5-6页 |
| 摘要 | 第7-11页 |
| Abbreviation | 第11-19页 |
| Chapter Ⅰ:Introduction and literature review | 第19-43页 |
| 1.1 Millet production and yield in the world | 第19-21页 |
| 1.2 Utilization of Millet | 第21-26页 |
| 1.2.1. Utilization of millet as food | 第21-24页 |
| 1.2.2 Utilization of millet in food industry | 第24-25页 |
| 1.2.3 Utilization of millet as animal feed and seed | 第25-26页 |
| 1.3 Chemical composition of millet | 第26-28页 |
| 1.4 Antinutrients of millet | 第28-32页 |
| 1.4.1 Total phenolic content,flavonoid content,phytic acid and phytate | 第28-29页 |
| 1.4.2 Phenolic acid composition of millet | 第29-32页 |
| 1.5 Function of millets | 第32-38页 |
| 1.5.1 Function of millet protein | 第32-38页 |
| 1.5.1.1 Millet protein on food intake,body and liver weight | 第32-35页 |
| 1.5.1.2 Millet protein on serum enzyme activities,plasma and liver cholesterols | 第35-38页 |
| 1.5.2 Function of millet starch | 第38页 |
| 1.6 Gut microbiota and health | 第38-41页 |
| 1.7 The aim of the study | 第41-43页 |
| 1.7.1 The general research technique routes | 第42-43页 |
| ChapterⅡ:Protein Extraction | 第43-56页 |
| 2.1 Introduction | 第43-44页 |
| 2.2 Materials and Methods | 第44-48页 |
| 2.2.1 Chemicals and equipment | 第44-45页 |
| 2.2.2 Pre-processing of foxtail millet | 第45页 |
| 2.2.3 Grinding of foxtail millet | 第45-46页 |
| 2.2.4 Preparation of protein concentration | 第46-47页 |
| 2.2.4.1 Protein concentration by enzymatic method | 第46页 |
| 2.2.4.1.1 Defatting of resultant material | 第46页 |
| 2.2.4.2 Protein concentration by prolamin extraction | 第46-47页 |
| 2.2.5 Cooking of FM diet | 第47页 |
| 2.2.6 Sensory evaluation of FM diet | 第47-48页 |
| 2.2.7 Proximate analysis of FM diet | 第48页 |
| 2.2.8 Statistical analysis | 第48页 |
| 2.3 Results and discussion | 第48-55页 |
| 2.3.1 Yield of concentrated protein and protein level | 第48-49页 |
| 2.3.2 Amino acid analysis of FM diet | 第49-51页 |
| 2.3.3 Cooking time and temperature effect on nutrition | 第51-54页 |
| 2.3.4 Sensory evaluation of FM diet | 第54-55页 |
| 2.4 Conclusion | 第55-56页 |
| Chapter Ⅲ:Effect of foxtail millet diet on liver injury and blood components in mice | 第56-81页 |
| 3.1 Introduction | 第56-57页 |
| 3.2 Materials and Method | 第57-63页 |
| 3.2.1 Chemicals and equipments | 第57-58页 |
| 3.2.2 Preparation and use of D-galactosamine dose | 第58-59页 |
| 3.2.3 Pre-processing and grinding of Foxtail millet | 第59页 |
| 3.2.4 Preparation of protein concentration | 第59页 |
| 3.2.5 Cooking and proximate analysis of extracted protein | 第59页 |
| 3.2.6 Animal and diet | 第59-60页 |
| 3.2.7 Nutritional and antinutritional composition | 第60-61页 |
| 3.2.8 Enrichment of extracted protein | 第61页 |
| 3.2.9 Preparation of dietary feed stick | 第61页 |
| 3.2.10 Investigation of data | 第61-62页 |
| 3.2.10.1 Food intake | 第61-62页 |
| 3.2.10.2 Body weight and weight gain | 第62页 |
| 3.2.10.3 Liver and relative liver weight | 第62页 |
| 3.2.11 Collection of blood | 第62页 |
| 3.2.12 Preparation of buffer | 第62页 |
| 3.2.13 Crushing of liver | 第62-63页 |
| 3.2.14 Biochemical analysis | 第63页 |
| 3.2.15 Histopathological analysis of liver | 第63页 |
| 3.2.16 Statistical analysis | 第63页 |
| 3.3 Results and discussion | 第63-81页 |
| 3.3.1 Sensory evaluation of dietary feed stick | 第63-64页 |
| 3.3.2 Antinutritional effect of FM diet | 第64-65页 |
| 3.3.3 Effect of FM diet on food intake, body weight, liver and relative liver weight in mice | 第65-69页 |
| 3.3.4 Effect of FM diet on serum enzyme activities in mice | 第69-72页 |
| 3.3.5 Effect of FM diet on plasma and liver cholesterols in mice | 第72-78页 |
| 3.3.6 Histopathological evaluation of liver | 第78-80页 |
| 3.3.7 Conclusion | 第80-81页 |
| Chapter Ⅳ:Effcet of FM diet on gut microbiology | 第81-97页 |
| 4.1 Introduction | 第81-83页 |
| 4.2 Materials and Methods | 第83-86页 |
| 4.2.1 Chemicals and equipments | 第83-84页 |
| 4.2.2 Microbial diversity analysis | 第84-85页 |
| 4.2.2.1 Fecal sample collection | 第84页 |
| 4.2.2.2 Fecal sample analysis | 第84页 |
| 4.2.2.3 DNA extraction and PCR amplification | 第84-85页 |
| 4.2.2.4 Illumina MiSeq sequencing | 第85页 |
| 4.2.2.5 Processing of sequencing data | 第85页 |
| 4.2.3 Metastats analysis | 第85-86页 |
| 4.3 Results and discussion | 第86-97页 |
| 4.3.1 Metrics | 第86页 |
| 4.3.2 Population analysis-OTUs approach | 第86-89页 |
| 4.3.3 Nonmetric multidimensional scaling(NMDS)analysis | 第89-90页 |
| 4.3.4 Impact of FM diet on gut microbiota | 第90-96页 |
| 4.3.5 Conclusion | 第96-97页 |
| Chapter Ⅴ:Conclusion and suggestions | 第97-100页 |
| 5.1 Conclusion | 第97-98页 |
| 5.2 Suggestions | 第98-100页 |
| References | 第100-124页 |
| ACKNOWLEDGEMENTS | 第124-126页 |
| ABOUT AUTHOR | 第126-131页 |
