| DEDICATION | 第1-6页 |
| ACKNOWLEDGEMENTS | 第6-7页 |
| ABSTRACT (English) | 第7-11页 |
| ABSTRACT (Chinese) | 第11-13页 |
| TABLE OF CONTENTS | 第13-17页 |
| LIST OF ABBREVIATIONS | 第17-18页 |
| Chapter 1: INTRODUCTION AND LITERATURE REVIEW | 第18-42页 |
| ·Indigestible dietary fibers and physiological aspect | 第19-25页 |
| ·Insoluble dietary fibers | 第20-21页 |
| ·Soluble dietary fibers | 第21-25页 |
| ·Pectins | 第21-22页 |
| ·Guar gum | 第22页 |
| ·Insulin and oligofructose | 第22-23页 |
| ·Polydextrose | 第23-24页 |
| ·Fibersol-2 | 第24-25页 |
| ·Function of Dietary fiber | 第25-27页 |
| ·Effect of dietary fiber on glucose absorption | 第25页 |
| ·Relationship between dietary fiber and cancer | 第25-27页 |
| ·Product specifications of Fibersol-2 | 第27页 |
| ·Effect of dietary fiber on mineral absorption | 第27-32页 |
| ·Characteristics of fibersol-2 | 第27-29页 |
| ·Effect of fibersol-2 on mineral absorption | 第29-31页 |
| ·Food applications of fibersol-2 | 第31-32页 |
| ·CURRENT PROBLEMS AN GENERAL OBJECTIVES | 第32-33页 |
| ·SPECIFIC OBJECTIVES OF THIS RESEARCH | 第33-34页 |
| ·REFERENCES | 第34-42页 |
| Chapter 2: LABORATORY PREPARATION OF FIBERSOL-2 USING COLUMN CHROMATOGRAPHY FOR DESALTING | 第42-61页 |
| ·INTRODUCTION | 第42页 |
| ·MATERIALS AND METHODS | 第42-48页 |
| ·Materials | 第42-43页 |
| ·Methods | 第43-48页 |
| ·Pretreatment and enzymatic hydrolysis of cornstarch | 第43页 |
| ·Preparation of activated charcoal for decolorization | 第43页 |
| ·Ions ex-change chromatography for desalting | 第43-44页 |
| ·Molecular weight analysis of the prepared fibersol-2 by high-performance liquid chromatography (HPLC) | 第44页 |
| ·Measuring total dietary fiber in the prepared fibersol-2 | 第44-47页 |
| ·Method for laboratory preparation of fibersol-2 | 第47-48页 |
| ·RESULTS AND DISCUSSION | 第48-59页 |
| ·Glucose removal effect of the hydrolysate pyrodextrin | 第48-50页 |
| ·Molecular weight distribution of the prepared fibersol-2 after using column chromatography for desalting, compared to the standard fiberso-2 | 第50-57页 |
| ·Total dietary fiber determination (TDF) | 第57-59页 |
| ·CONCLUSION | 第59页 |
| ·REFERENCES | 第59-61页 |
| Chapter 3: LABORATORY PREPARATION OF FTBERSOL-2 USING SACCHAROMYCES CEREVISIAE FOR DESALTING | 第61-75页 |
| ·INTRODUCTION | 第61页 |
| ·MATERIALS AND METHODS | 第61-63页 |
| ·Materials | 第61页 |
| ·Methods | 第61-63页 |
| ·Pretreatment and enzymatic hydrolysis of cornstarch | 第61-62页 |
| ·Yeast fermentation | 第62页 |
| ·Molecular weight analysis of the prepared fibersol-2 by high- performance liquid chromatography (HPLC) | 第62-63页 |
| ·Measuring total dietary fiber in the prepared fibersol-2 | 第63页 |
| ·Statistical analysis | 第63页 |
| ·RESULTS AND DISCUSSION | 第63-72页 |
| ·Yeast application effect for desalting fibersol-2 | 第63-71页 |
| ·Molecular weight distribution of the prepared fibersol-2 after using yeast for desalting, compared to the standard fiberso-2 | 第71-72页 |
| ·CONCLUSION | 第72-73页 |
| ·REFERENCES | 第73-75页 |
| Chapter 4: STUDY OF CALCIUM BINDING EFFECT ON FIBERSOL-2 | 第75-88页 |
| ·INTRODUCTION | 第75页 |
| ·MATERIALS AND METHODS | 第75-81页 |
| ·Materials | 第75-76页 |
| ·Methods | 第76-81页 |
| ·Pretreatment and Enzymatic Hydrolysis | 第76页 |
| ·Calcium association with fibersol-2 | 第76页 |
| ·Desalting fibersol-2 | 第76页 |
| ·Determination of total calcium content by atomic absorption spectrophotometer measurement | 第76-77页 |
| ·Determination of free calcium content by ion selective electrode potentiometer measurement | 第77页 |
| ·Viscosity analysis of fibersol-2 with and without calcium binding | 第77-79页 |
| ·Method for laboratory preparation of calcium fortified fibersol-2 | 第79-80页 |
| ·Sensory evaluation | 第80页 |
| ·Product specifications and analytical methods of fibersol-2 | 第80页 |
| ·Statistical analysis | 第80-81页 |
| ·RESULTS AND DISCUSSION | 第81-85页 |
| ·Specification of the prepared fibersol-2 | 第81-83页 |
| ·Fibersol-2 fortification with calcium | 第83-84页 |
| ·Sensory evaluation | 第84-85页 |
| ·CONCLUSION | 第85-86页 |
| ·REFERENCES | 第86-88页 |
| Chapter 5: IN-VIVO EVALUATION OF LABORATORY PREPARED FIBERSOL-2 AND CALCIUM CHELATED FIBERSOL-2 | 第88-101页 |
| ·INTRODUCTION | 第88-89页 |
| ·MATERIALS AND METHODS | 第89-91页 |
| ·Materials | 第89页 |
| ·Methods | 第89-91页 |
| ·Animal grading and metabolism test of calcium chelated to fibersol-2 | 第89-90页 |
| ·Blood glucose level and insulin secretion analysis | 第90-91页 |
| ·Statistical analysis | 第91页 |
| ·RESULTS AND DISCUSSION | 第91-98页 |
| ·Content of the laboratory prepared fibersol-2 and laboratory prepared calcium fortified fibersol-2 in feces and urine after feeding mice | 第91-95页 |
| ·Effect of absorbed and retained calcium after feeding mice with laboratory prepared calcium fortified fibersol-2 | 第95-96页 |
| ·Effect of the laboratory prepared fibersol-2 and laboratory prepared calcium fortified fibersol-2 on blood glucose level and insulin secretion | 第96-98页 |
| ·CONCLUSION | 第98页 |
| ·REFERENCES | 第98-101页 |
| Chapter 6: FOOD APPLICATION OF LABORATORY PREPARED FD3ERSOL-2 AND CALCIUM CHELATED FIBERSOL-2:- Cake | 第101-111页 |
| ·INTRODUCTION | 第101-102页 |
| ·MATERIALS AND METHODS | 第102-105页 |
| ·Materials | 第102页 |
| ·Methods | 第102-105页 |
| ·Sensory evaluation | 第104页 |
| ·Statistical analysis | 第104-105页 |
| ·RESULTS AND DISCUSSION | 第105-109页 |
| ·CONCLUSION | 第109页 |
| ·REFERENCES | 第109-111页 |
| GENERAL CONCLUSION AND RECOMMENDATIONS | 第111-114页 |
| 1. GENERAL CONCLUSION | 第111-112页 |
| 2. RECOMMENDATIONS | 第112-114页 |
| PAPERS PUBLISHED | 第114-115页 |
| APPENDIX | 第115-125页 |
