| DEDICATION | 第1-6页 |
| List of Figures | 第6-7页 |
| List of Tables | 第7-8页 |
| List of Abbreviations | 第8-9页 |
| Acknowledgement | 第9-10页 |
| ABSTRACT | 第10-12页 |
| 摘要 | 第12-14页 |
| Tables of content | 第14-18页 |
| PART ONE: GENERAL INTRODUCTION AND REVIEW OF RELEVANT LITERATURE | 第18-32页 |
| ·General introduction | 第18-19页 |
| ·Literature review | 第19-30页 |
| ·Polyphenols | 第19-20页 |
| ·Polyphenols and Methylxanthines in cocoa | 第20-21页 |
| ·Extraction of polyphenols | 第21-22页 |
| ·Quantification of polyphenols | 第22-23页 |
| ·Measurement of antioxidant activity of polyphenols | 第23页 |
| ·Bioactivity and mechanisms of Polyphenols and their influence on human health | 第23-24页 |
| ·Bioavailability and Pharmaceutical potential of cocoa polyphenols | 第24-25页 |
| ·Dietary Fiber | 第25-30页 |
| ·Definition and Composition | 第25-27页 |
| ·Sources and Functional properties of DF | 第27页 |
| ·Physicochemical Properties | 第27-28页 |
| ·Physiological Properties | 第28-30页 |
| ·Antioxidant Activity | 第30页 |
| ·Problem statement and study justification | 第30-32页 |
| ·Main objective | 第31页 |
| ·Specific objective | 第31-32页 |
| PART TWO: MATERIAL AND METHOD | 第32-47页 |
| ·Plant materials | 第32-33页 |
| ·Chemicals | 第32页 |
| ·Proximate analysis | 第32-33页 |
| ·In vitro Antioxidant and antimicrobial activities of CBS phenolic extracts | 第33-34页 |
| ·Sample preparations and polphenols Extraction | 第33页 |
| ·Determination of Total phenolic (TP) and Total Flavonoids (TF) contents | 第33-34页 |
| ·Other compositional analysis of CBS polyphenolic extracts | 第34页 |
| ·In vitro antioxidant measurements | 第34-36页 |
| ·2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging assay | 第34-35页 |
| ·β- carotene linoleic acid assay | 第35-36页 |
| ·Ferric reducing/antioxidant power (FRAP) assay | 第36页 |
| ·Antimicrobial investigations | 第36-38页 |
| ·Bacterial strains and preparation of the standardized bacterial suspension | 第36-37页 |
| ·Sample preparation for antibacterial activity | 第37页 |
| ·Disk diffusion method | 第37页 |
| ·Broth microdilution method | 第37-38页 |
| ·HPLC analysis of major phenolic compounds | 第38-39页 |
| ·Physicochemical, and Biological Functional properties of CBS dietary fibers | 第39-40页 |
| ·Preparation of Dietary fiber Powder Concentrates | 第39页 |
| ·Proximate analysis and monosaccharide analysis SDF, IDF and TDF | 第39-40页 |
| ·Physicochemical functions analyses of CBS fiber powders/concentrates | 第40-41页 |
| ·Analysis of physical characteristics | 第40页 |
| ·Color | 第40页 |
| ·Bulk and Tapped Density | 第40页 |
| ·Porosity | 第40-41页 |
| ·Viscosity determination | 第41页 |
| ·Analysis of hydration properties: | 第41页 |
| ·Water Holding and swelling Capacities (WHC) | 第41页 |
| ·Investigations of Biological Functional properties of CBS Powder Concentrates | 第41-44页 |
| ·Hypoglycemic and Cholesterol lowering effects of CBS fibers | 第41页 |
| ·Measurement of glucose adsorption capacity (GAC) of CBS fibers | 第41-42页 |
| ·Glucose dialysis retardation Index measurement (GDRI) | 第42页 |
| ·Determination of fiber effect on Starch Digestibility | 第42-43页 |
| ·Measurement of dietary fiber binding capacity for cholesterol in egg yolk | 第43页 |
| ·Measurement of fat/oil binding capacity | 第43-44页 |
| ·Determination of sodium cholate absorption capacity | 第44页 |
| ·Antioxidant Activity and total polyphenolic content measurement | 第44-45页 |
| ·2, 2-diphenyl-1-picrylhydrazylradical (DPPH) scavenging assay | 第44-45页 |
| ·2, 2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) | 第45页 |
| ·Measurement of Total phenolic (TPC) and Total Flavanoid content TFC) | 第45-46页 |
| ·HPLC analysis of major phenolic compounds | 第46页 |
| ·Statistical Treatment | 第46-47页 |
| PART THREE: RESULTS AND DISCUSSION | 第47-73页 |
| ·Proximate analysis | 第47页 |
| ·Antioxidant and antimicrobial activities of CBS Phenolic extracts | 第47-54页 |
| ·Total phenolic and contents other compositional analysis of extracts | 第47-48页 |
| ·Scavenging activity on DPPH radical | 第48-51页 |
| ·β-Carotene–linoleate assay bleaching | 第51-53页 |
| ·Ferric reducing activity based on FRAP assay | 第53-54页 |
| ·Antimicrobial activities | 第54-56页 |
| ·Antimicrobial activities as related to the diameter of zone of inhibition | 第54-55页 |
| ·Minimum Inhibitory Concentration (MIC) of Extracts | 第55-56页 |
| ·Identification and quantification of major compounds | 第56-58页 |
| ·Potential Biological and Physicochemical Properties and of CBS Dietary Fibers | 第58-61页 |
| ·Proximate analysis | 第58-59页 |
| ·Monosaccharide Composition and Uronic acid content of CBS Fibers | 第59-61页 |
| ·Physicochemical Properties of CBS fiber powder concentrates | 第61-62页 |
| ·Physical properties | 第61页 |
| ·Bulk, Tapered Densities and Porosities | 第61-62页 |
| ·Physicochemical properties | 第62-64页 |
| ·Physiological properties of CBS fibers | 第64-70页 |
| ·Viscosity | 第64-65页 |
| ·Glucose adsorption capacity (GAC) of CBS fiber fractions | 第65-66页 |
| ·Effects of CBS fibers on Diffusion of Glucose | 第66-68页 |
| ·Effect of CBS fibers on α-Amylase Kinetics /Starch Digestibility | 第68-69页 |
| ·Binding capacity of CBS fiber powders for oil, cholesterol, and cholic acid | 第69-70页 |
| ·Antioxidant and phenolic content of CBS fiber concentrates | 第70-72页 |
| ·HPLC analysis identification of bioactive compounds | 第72-73页 |
| PART FOUR: GENERAL CONCLUSIONS AND RECOMMENDATIONS | 第73-75页 |
| ·Conclusion | 第73-74页 |
| ·Recommendations | 第74-75页 |
| 附件 | 第75-76页 |
| List of References | 第76-83页 |
| Annex | 第83-85页 |
