| 摘要 | 第9-12页 |
| Abstract | 第12-14页 |
| 1 Introduction | 第15-36页 |
| 1.1 Research Background | 第15-16页 |
| 1.2 Literature review | 第16-36页 |
| 1.2.1 Lactic acid bacteria | 第16-18页 |
| 1.2.2 Lactic Acid Bacteria (LAB) and Probiotic | 第18-22页 |
| 1.2.3 Lactobacillus brevis | 第22-24页 |
| 1.2.4 Freeze-dried technique | 第24页 |
| 1.2.5 γ- Aminobutyric acid (GABA) | 第24-27页 |
| 1.2.6 Diabetes | 第27-34页 |
| 1.2.7 Streptozotocin (STZ) and diabetic induction | 第34-36页 |
| 2 Materials and Methods | 第36-47页 |
| 2.1 Experimental Materials | 第36-38页 |
| 2.1.1 Test strain | 第36页 |
| 2.1.2 Cell line and cell culture medium | 第36页 |
| 2.1.3 Medium | 第36-37页 |
| 2.1.4 Experimental animals | 第37页 |
| 2.1.5 Main reagents and sources | 第37页 |
| 2.1.6 Main experimental apparatus | 第37-38页 |
| 2.2 Experimental methods | 第38-47页 |
| 2.2.1 Bacterial strains and cultivation conditions | 第38-39页 |
| 2.2.2 Glutamic acid decarboxylase (gad) Gene Library Screening of Lactobacillus brevis strains | 第39页 |
| 2.2.3 PCR amplification of the 16S rDNA and sequence analysis | 第39页 |
| 2.2.4 Freeze-dried Lactobacillus brevis strains with cryoprotective agents | 第39-40页 |
| 2.2.5 Assessment of KLDS 1.0727 and KLDS 1.0373 in vitro | 第40-44页 |
| 2.2.6 In vivo experiments | 第44-47页 |
| 3 Results and Analysis | 第47-75页 |
| 3.1 The presence of glutamic acid decarboxylase (gad) gene in Lactobacillus brevis strains | 第47-48页 |
| 3.2 Tolerance of KLDS 1.0727 and KLDS 1.0373 to simulation (GIJ) in vitro | 第48-62页 |
| 3.2.1 Determination of growth rate and acidification of Lactobacillus brevis strains | 第48-49页 |
| 3.2.2 Survival of Lactobacillus brevis strains in acidic conditions | 第49页 |
| 3.2.3 The resistance of free or freeze-dried KLDS 1.0727 and KLDS 1.0373 to bile salts in MRSincubated at 37℃ | 第49页 |
| 3.2.4 Resistance of KLDS 1.0727 and KLDS 1.0373 to different types of antibiotics | 第49-52页 |
| 3.2.5 Tolerance of KLDS 1.0727 and KLDS 1.0373 to simulated intestinal juice (SIJ) with differentp H levels under aerobic or anaerobically conditions | 第52-55页 |
| 3.2.6 Survival of free or freeze-dried Lactobacillus brevis strains in simulated (GIJ) conditions | 第55-58页 |
| 3.2.7 Inhibitory effect of free or freeze-dried KLDS 1.0727 and 1.0373 strains against foodbornepathogenic bacteria | 第58-61页 |
| 3.2.8 Adhesion of KLDS 1.0727 and KLDS 1.0373 to Caco-2 cells | 第61-62页 |
| 3.3 Intervention Effect of KLDS 1.0727 and KLDS 1.0373 strains on Type I Diabetes in vivo | 第62-75页 |
| 3.3.1 Hypoglycemic Activity and body weight of C57BL/6 Mice with Streptozotocin (STZ) InducedDiabetes during four weeks | 第62-66页 |
| 3.3.2 Serum biochemistry parameters | 第66-68页 |
| 3.3.3 Liver functions parameters | 第68-70页 |
| 3.3.4 Kidney function parameter | 第70-72页 |
| 3.3.5 Blood plasma insulin analysis | 第72页 |
| 3.3.6 Histology of mice organs streptozotocin-induced diabetes | 第72-75页 |
| 4 Discussions | 第75-82页 |
| 4.1 Screening and identification of Lactobacillus brevis strains for the presence of glutamic aciddecarboxylase (gad) gene | 第75页 |
| 4.2 Determine resistance of KLDS 1.0727 and KLDS 1.0373 free or freeze-dried to differentconcentration of bile salts in acidic conditions | 第75-76页 |
| 4.3 The resistance of KLDS 1.0727 and KLDS 1.0373 for different types of antibiotics | 第76页 |
| 4.4 Tolerances of KLDS 1.0727 and KLDS 1.0373 to simulated intestinal juice (SIJ) with differentp H in aerobic or anaerobic conditions and simulated gastric juice (GIT) conditions | 第76-77页 |
| 4.5 antagonistic properties of free or freeze-dried KLDS 1.0727 and KLDS 1.0373 strains againstfoodborne pathogenic bacteria | 第77页 |
| 4.6 Caco-2 cell adhesion assay | 第77页 |
| 4.7 In vivo discussions | 第77-82页 |
| 5 Conclusion | 第82-83页 |
| Acknowledgements | 第83-84页 |
| References | 第84-97页 |
| Appendix list of abbreviations | 第97-98页 |
| Paper published in the period of Ph.D. education | 第98页 |
