| Acknowledgement | 第5-6页 |
| Abbreviations | 第6-13页 |
| Abstract | 第13-14页 |
| 摘要 | 第15-21页 |
| Chapter 1. Introduction and Literature Review | 第21-48页 |
| 1.1. General introduction | 第21-22页 |
| 1.2. Review of Relevant Literature | 第22-40页 |
| 1.2.1. Stilbenes | 第22-29页 |
| 1.2.2. Fluoroquinolones | 第29-34页 |
| 1.2.3. Production of Monoclonal Antibody | 第34-40页 |
| 1.3. Statement of the Problem and Justification of Research | 第40-41页 |
| 1.4. Objectives of the Study | 第41-42页 |
| 1.4.1. Main Objective | 第41页 |
| 1.4.2. Specific Objectives | 第41-42页 |
| 1.5. References | 第42-48页 |
| Chapter 2. Development of an Immunochromatographic Assay for the Detection of Hexestrol andDiethylstilbestrol Residues in Bovine Milk Sample | 第48-68页 |
| 2.1. Introduction | 第48-49页 |
| 2.2. Materials and Methods | 第49-57页 |
| 2.2.1. Chemicals and materials | 第49-50页 |
| 2.2.2. Hapten synthesis and Immunogen preparation | 第50-52页 |
| 2.2.3. Immunization | 第52-53页 |
| 2.2.4. Production of monoclonal antibodies | 第53页 |
| 2.2.5. Development of Indirect Competitive ELISA | 第53-55页 |
| 2.2.6. Development of Immunochromatographic assay test kit: | 第55-56页 |
| 2.2.7. Recovery test in Milk | 第56-57页 |
| 2.3. Results and discussion | 第57-65页 |
| 2.3.1. Hapten, Immunogen and coating antigen synthesis | 第57-59页 |
| 2.3.2. Production of monoclonal antibodies | 第59页 |
| 2.3.3. Optimization of ic-ELISA | 第59-61页 |
| 2.3.4. Sensitivity and Specificity of anti-HES monoclonal antibody on ic-ELISA assay | 第61-62页 |
| 2.3.5. Sensitivity of Immunochromatographic assay | 第62-63页 |
| 2.3.6. Performance of ic-ELISA and LFA in Milk | 第63-65页 |
| 2.4. Conclusion | 第65页 |
| 2.5. References | 第65-68页 |
| Chapter 3. Rapid and Sensitive Immunoassays for the Detection of Lomefloxacin and RelatedDrug Residues in Bovine Milk Samples | 第68-86页 |
| 3.1. Introduction | 第68-69页 |
| 3.2. Materials and methods | 第69-74页 |
| 3.2.1. Chemicals and instruments | 第69-70页 |
| 3.2.2. Antigen design and synthesis | 第70-71页 |
| 3.2.3. Immunization and antibody production | 第71-72页 |
| 3.2.4. Cell fusion, Screening and Antibody production | 第72页 |
| 3.2.5. Ic-ELISA development and optimization | 第72-73页 |
| 3.2.6. Recovery studies by ic-ELISA | 第73页 |
| 3.2.7. Synthesis of GNP-labeled mAb | 第73-74页 |
| 3.2.8. Immunochromatographic strip preparation | 第74页 |
| 3.2.9. Application of the strip device | 第74页 |
| 3.3. Results and Discussion | 第74-81页 |
| 3.3.1. Antigen synthesis | 第74-75页 |
| 3.3.2. Mice immunity evaluation | 第75-76页 |
| 3.3.3. Optimization and specificity of ic-ELISA | 第76-78页 |
| 3.3.4. Cross-reactivity study and establishment of standard curves | 第78-79页 |
| 3.3.5. Recovery results of spiked bovine milk samples by ic-ELISA | 第79页 |
| 3.3.6. Lateral flow immunochromatographic assay | 第79-81页 |
| 3.4. Conclusion | 第81-82页 |
| 3.5. References | 第82-86页 |
| Chapter 4. Development of an Indirect Enzyme-Linked Immunosorbent Assay (Ic-Elisa) and Lateral-Flow Test Strips to Detect the Residues of Pefloxacin and its Analogues in Chicken Muscle | 第86-107页 |
| 4.1. Introduction | 第86-87页 |
| 4.2. Materials and Methods | 第87-94页 |
| 4.2.1. Reagents and Apparatus | 第87-88页 |
| 4.2.2. Design and synthesis of antigens | 第88-89页 |
| 4.2.3. Immunization of mice | 第89-90页 |
| 4.2.4. Cell fusion, screening and production of mAb | 第90页 |
| 4.2.5. ELISA procedure | 第90-91页 |
| 4.2.6. Cross-reactivity determination | 第91页 |
| 4.2.7. Preparation of the lateral-flow test strip | 第91-92页 |
| 4.2.8. Sample preparation and analysis | 第92-93页 |
| 4.2.9. Application of the lateral flow test strip in 0.01 M PBS and spiked chicken meat | 第93-94页 |
| 4.3. Results and Discussion | 第94-102页 |
| 4.3.1. Design and synthesis of the coating antigen | 第94-95页 |
| 4.3.2. Mice blood Testing | 第95页 |
| 4.3.3. Optimization of ic-ELISA | 第95-98页 |
| 4.3.4. Determination of cross-reactivity | 第98-99页 |
| 4.3.5. Immunoassay analysis of spiked chicken muscle samples | 第99-100页 |
| 4.3.6. Lateral-flow strip detection of FQs in 0.01 M PBS and spiked samples | 第100-102页 |
| 4.4. Conclusion | 第102-103页 |
| 4.5. References | 第103-107页 |
| Conclusions and Recommendations | 第107-110页 |
| General Conclusions | 第107-108页 |
| Key Innovations | 第108-109页 |
| RECOMMENDATIONS | 第109-110页 |
| PUBLICATIONS & PATENTS | 第110-111页 |
| Published Articles | 第110页 |
| Co-authored Articles | 第110-111页 |
| Patented works | 第111页 |
