| 摘要 | 第1-6页 |
| Abstract | 第6-11页 |
| Chapter 1 General Introduction and objectives of this thesis | 第11-25页 |
| ·Physiological properties and clinical application of adenosine | 第11-13页 |
| ·Role of adenosine in the heart | 第11-12页 |
| ·Role of adenosine in the nervous system | 第12页 |
| ·Role of adenosine in the skeletal muscle | 第12-13页 |
| ·Importance of nucleosides, nucleotides and related compounds | 第13页 |
| ·De novo biosynthesis of purine nucleotides and nucleosides | 第13-14页 |
| ·Regulation of purine nucleotides production | 第14-16页 |
| ·Methods for the production of nucleic acid-related substances | 第16-21页 |
| ·Enzymatic hydrolysis of RNA | 第17-18页 |
| ·Fermentation production of 5'-IMP | 第18页 |
| ·Fermentation production of inosine | 第18-19页 |
| ·Fermentation production of guanosine | 第19页 |
| ·Fermentation production of adenosine and adenine | 第19-21页 |
| ·Optimization procedures | 第21-23页 |
| ·Empirical optimization | 第21页 |
| ·Statistically-based optimization | 第21-22页 |
| ·Analysis of quadratic models | 第22-23页 |
| ·Objectives of this thesis | 第23-25页 |
| Chapter 2 Materials and Methods | 第25-31页 |
| ·Equipments | 第25页 |
| ·Chemical reagents | 第25-26页 |
| ·Bacterial strain | 第26页 |
| ·Culture media | 第26页 |
| ·Preparation of seed culture | 第26页 |
| ·Fermentation conditions | 第26-27页 |
| ·Cell growth measurement | 第27页 |
| ·Residual sugar determination | 第27-28页 |
| ·Quantitative determination of bases and nucleosides | 第28-29页 |
| ·HPLC assay of adenosine | 第29-31页 |
| Chapter 3 Results and Discussions | 第31-62页 |
| ·Evaluation of factors affecting adenosine accumulation | 第31-33页 |
| ·Effect of corn steep liquor and yeast extract on growth and adenosine production | 第33-41页 |
| ·Culture conditions | 第34页 |
| ·Experimental design | 第34-35页 |
| ·Data analysis for cell growth | 第35-37页 |
| ·Data analysis for adenosine accumulation | 第37-41页 |
| ·Model validation | 第41-43页 |
| ·Strain preservation | 第43-44页 |
| ·Action of antibiotics and surface active agents on adenosine production | 第44-57页 |
| ·Culture conditions | 第44页 |
| ·Effect of antibiotics | 第44-49页 |
| ·Effect of surface active agents | 第49-53页 |
| ·Response surface representation of the effects of Tween 80 and time of addition | 第53-57页 |
| ·Effects of additional medium components | 第57-62页 |
| ·Magnesium phosphate | 第57-59页 |
| ·EDTA | 第59页 |
| ·Sodium citrate | 第59-60页 |
| ·Effect of magnesium sulfate concentration | 第60-62页 |
| Chapter 4 Conclusions and suggestions for future work | 第62-64页 |
| References | 第64-70页 |
| Acknowledgments | 第70页 |
