| 摘要 | 第6-7页 |
| ABSTRACT | 第7-8页 |
| LIST OF ABBREVIATIONS | 第13-16页 |
| CHAPTER1 INTRODUCTION | 第16-38页 |
| 1.1 GENERAL INTRODUCTION | 第16-18页 |
| 1.2 REVIEW OF LITERATURE | 第18-38页 |
| 1.2.1 Lesion mimic mutants | 第18-20页 |
| 1.2.2 Hypersensitive response and programmed cell death | 第20-21页 |
| 1.2.3 LMM and systemic acquired resistance(SAR) | 第21-22页 |
| 1.2.4 Factors affecting development of lesion mimic phenotype | 第22-23页 |
| 1.2.5 Enhanced defense responses and disease resistance in LMMs | 第23页 |
| 1.2.6 Benefits of studying LMM | 第23-24页 |
| 1.2.7 Rice as a model for studying LMM | 第24-25页 |
| 1.2.8 Rice LMMs identified and studied so far | 第25-32页 |
| 1.2.9 Bacterial blight disease of rice | 第32-33页 |
| 1.2.10 LMM/spl mutants with resistance to bacterial blight of rice | 第33-35页 |
| 1.2.11 Advanced gene mapping with high-throughput resequencing methods | 第35-36页 |
| 1.2.12 Mediator:a master transcriptional regulator | 第36-38页 |
| CHAPTER2 Agronomic and Phenotypic Characterization of spl40 | 第38-53页 |
| 2.1 INTRODUCTION | 第38-39页 |
| 2.2 MATERIALS AND METHODS | 第39-44页 |
| 2.2.1 Plant materials and growth conditions | 第39页 |
| 2.2.2 Photosynthetic pigment content measurement | 第39-40页 |
| 2.2.3 Total soluble protein content measurement | 第40-41页 |
| 2.2.4 Measurement of photosynthesis related parameters | 第41页 |
| 2.2.5 Preperation of RNA | 第41-42页 |
| 2.2.6 Synthesis of cDNA | 第42-43页 |
| 2.2.7 Quantitative-Real-time PCR(qRT-PCR) | 第43-44页 |
| 2.2.8 Shading experiment | 第44页 |
| 2.2.9 Statistical analysis | 第44页 |
| 2.3 RESULTS | 第44-50页 |
| 2.3.1 Phenotypic performance of spl | 第44-46页 |
| 2.3.2 Reductions in photosynthetic pigment and soluble protein content | 第46-47页 |
| 2.3.3 Altered photosynthetic capacity in spl | 第47-48页 |
| 2.3.4 Downregulated expression of photosynthesis related genes in spl40 | 第48-49页 |
| 2.3.5 Lesion initiation was light-dependent | 第49-50页 |
| 2.4 DISCUSSION | 第50-52页 |
| 2.5 CONCLUSION | 第52-53页 |
| CHAPTER3 Analysis of Cell Death Occurrence,ROS Production and Evaluation of Bacterial Blight Resistance in spl40 | 第53-70页 |
| 3.1 INTRODUCTION | 第53-54页 |
| 3.2 MATERIALS AND METHODS | 第54-60页 |
| 3.2.1 Histochemical analyses | 第54-55页 |
| 3.2.1.1 Evans blue staining | 第54页 |
| 3.2.1.2 DAB staining | 第54-55页 |
| 3.2.1.3 NBT staining | 第55页 |
| 3.2.2 Measurement of physio-biochemical parameters | 第55-59页 |
| 3.2.2.1 Measurement of MDA content | 第55-56页 |
| 3.2.2.2 Measurement of CAT activity | 第56-57页 |
| 3.2.2.3 Measurement of SOD activity | 第57-58页 |
| 3.2.2.4 Measurement of POD activity | 第58-59页 |
| 3.2.3 Disease resistance evaluation | 第59-60页 |
| 3.2.4 Analysis of gene expression by qRT-PCR | 第60页 |
| 3.2.5 Statistical analysis | 第60页 |
| 3.3 RESULTS | 第60-67页 |
| 3.3.1 ROS accumulation and cell death occur in spl | 第60-63页 |
| 3.3.2 Perturbed ROS scavenging system in spl | 第63-65页 |
| 3.3.3 Enhanced resistance to bacterial blight pathogen | 第65-67页 |
| 3.4 DISCUSSION | 第67-69页 |
| 3.5 CONCLUSION | 第69-70页 |
| CHAPTER4 Genetic Mapping,Map-based Cloning and Functional Complementation of spl40 | 第70-105页 |
| 4.1 INTRODUCTION | 第70-71页 |
| 4.2 MATERIALS AND METHODS | 第71-91页 |
| 4.2.1 Genetic control analysis | 第71页 |
| 4.2.2 Gene mapping | 第71-73页 |
| 4.2.2.1 Primary mapping and fine mapping | 第71-72页 |
| 4.2.2.2 High-throughput sequencing to detect the candidate SNP | 第72页 |
| 4.2.2.3 Confirmation of the candidate SNP | 第72-73页 |
| 4.2.3 Cloning of candidate gene and vector construction for functional complementation | 第73-80页 |
| 4.2.3.1 PCR amplification of genomic fragments of Os05G0312000 | 第73-76页 |
| 4.2.3.2 TA cloning and sequencing of genomic fragments | 第76-79页 |
| 4.2.3.3 Cloning into pCAMBIA1300 vector | 第79-80页 |
| 4.2.4 Vector construction for overexpression of spl | 第80-83页 |
| 4.2.4.1 PCR amplification of full length coding sequence | 第80-81页 |
| 4.2.4.2 Vector construction | 第81-83页 |
| 4.2.5 Trangenic plant development via Agrobacterium tumefaciens-mediated plant transformation | 第83-84页 |
| 4.2.5.1 Transformation of plamsid vector into Agrobacterium tumefaciens by electroporation method | 第83页 |
| 4.2.5.2 Agrobacterium tumefaciens-mediated plant transformation and plant regeneration | 第83-84页 |
| 4.2.6 Protoplast isolation and subcellular localization | 第84-88页 |
| 4.2.6.1 Vector construction for subcellular localization | 第84-85页 |
| 4.2.6.2 Rice protoplast isolation | 第85-88页 |
| 4.2.7 Vector construction RNA interference(RNAi) | 第88-89页 |
| 4.2.8 Analysis of SPL40 gene expression in different parts | 第89-90页 |
| 4.2.9 Yeast two-hybrid assay to find the interacting protein | 第90-91页 |
| 4.3 RESULTS | 第91-102页 |
| 4.3.1 Genetic control of spl | 第91页 |
| 4.3.2 Physical mapping of spl40 mutation | 第91-93页 |
| 4.3.3 Gene annotation of Os05G0312000(LOC_Os05g24684) | 第93-94页 |
| 4.3.4 Functional complementation with WT allele | 第94-96页 |
| 4.3.5 spl40 allelic mutant spl40NIP | 第96-97页 |
| 4.3.6 Phenotype of F1s from different crosses | 第97-98页 |
| 4.3.7 Subcellular localization of SPL | 第98-99页 |
| 4.3.8 Expression of SPL40 in different tissues | 第99-100页 |
| 4.3.9 Identification of putative interacting proteins | 第100-102页 |
| 4.4 DISCUSSION | 第102-104页 |
| 4.5 CONCLUSION | 第104-105页 |
| CHAPTER5 Major Findings and Future Perspectives | 第105-106页 |
| 5.1 MAJOR FINDINGS | 第105页 |
| 5.2 FUTURE PERSPECTIVES | 第105-106页 |
| REFERENCES | 第106-115页 |
| APPENDIX | 第115-131页 |
| LIST OF PUBLICATIONS | 第131-132页 |
| ACKNOWLEDGEMENTS | 第132-133页 |
| AUTHORS RESUME | 第133页 |
