| 摘要 | 第3-6页 |
| Abstract | 第6-7页 |
| Chapter 1 Introduction | 第10-24页 |
| 1.1 Cytochrome P450s and Their Possible Role in Bone Biology | 第10-12页 |
| 1.1.1 Introduction of Cytochrome P450s | 第10-11页 |
| 1.1.2 The Role of CYPs in Bone Biology | 第11-12页 |
| 1.2 CYPs and Inflammation-Induced Osteoporosis | 第12-17页 |
| 1.2.1 Bone Remodeling | 第12-15页 |
| 1.2.2 CYPs Involved Bone Biology and Inflammation-induced Osteoporosis | 第15-17页 |
| 1.3 Crosstalk between Metastatic Breast Cancer and Bone Microenvironment | 第17-24页 |
| 1.3.1 Breast Cancer | 第17页 |
| 1.3.2 Bone Metastases | 第17-19页 |
| 1.3.3 Mechanisms of Osteolytic Metastasis | 第19-24页 |
| Chapter 2 Materials and Methods | 第24-32页 |
| 2.1 Materials and Reagents | 第24页 |
| 2.2 Instruments and Consumables | 第24-25页 |
| 2.3 Methods | 第25-32页 |
| 2.3.1 Cell Culture | 第25-28页 |
| 2.3.2 RNA Extraction and Reverse Transcription | 第28页 |
| 2.3.3 Quantitative Real-time PCR Analysis | 第28-30页 |
| 2.3.4 Statistical analysis | 第30页 |
| 2.3.5 Detailed Experimental Design | 第30-32页 |
| Chapter 3 Results | 第32-42页 |
| 3.1 Comparison of Cytochrome P450 Expression in Four Different HumanOsteoblast Models | 第32-34页 |
| 3.2 Cytochrome P450 Expression Pattern in Human Osteoblast duringOsteogenic Differentiation with or without TNF-α Treatment | 第34-39页 |
| 3.2.1 CYPs Gene Expression Pattern in MG63 and HOB during Different Stages ofOsteogenic Differentiation | 第34-37页 |
| 3.2.2 Effects of TNF-α on CYPs Gene Expression in MG63 and HOB | 第37-39页 |
| 3.3 The Role of CYPs in the Crosstalk Interaction between Human Osteoblastand Breast Cancer Cells | 第39-42页 |
| 3.3.1 MG63 Cells and HOB Cells Cultured in MCF-7 Conditioned Medium | 第39-40页 |
| 3.3.2 MCF-7 Cells Cultured in HOB Conditioned Medium | 第40-42页 |
| Chapter 4 Discussions | 第42-54页 |
| 4.1 Comparison of Cytochrome P450 Expression in Four Different HumanOsteoblast Models | 第42-47页 |
| 4.2 Cytochrome P450 Expression Pattern in Human Osteoblast duringOsteogenic Differentiation with or without TNF-α Treatment | 第47-51页 |
| 4.2.1 CYPs Gene Expression Pattern in MG63 and HOB during Different Stages ofOsteogenic Differentiation | 第49页 |
| 4.2.2 Effects of TNFα on CYPs Gene Expression in MG63 and HOB | 第49-50页 |
| 4.2.3 Time Dependent Influence of TNF-α on CYPs Expression | 第50-51页 |
| 4.3 The Role of CYPs in the Crosstalk Interaction between Human Osteoblastand Breast Cancer Cells | 第51-54页 |
| 4.3.1 MG63 Cells and HOB Cells-MCF-7 Conditioned Medium | 第51-52页 |
| 4.3.2 MCF-7 Cells-HOB Conditioned Medium | 第52-54页 |
| Chapter 5 Conclusions and Future Prospects | 第54-58页 |
| 5.1 Comparison of Cytochrome P450 Expression in Four Different HumanOsteoblast Models | 第54页 |
| 5.2 Cytochrome P450 Expression Pattern in Human Osteoblast duringOsteogenic Differentiation with or without TNF-α Treatment | 第54-55页 |
| 5.3.The Role of CYPs in the Crosstalk Interaction between Human Osteoblastand Breast Cancer Cells | 第55-58页 |
| References | 第58-74页 |
| Notes on Publications and Participation in Scientific Research | 第74-76页 |
| Acknowledgement | 第76-77页 |
