| 摘要 | 第4-9页 |
| ABSTRACT | 第9-10页 |
| CHAPTER1 Introduction | 第14-24页 |
| 1.1 The biology of IL-2 | 第14-15页 |
| 1.2 The biology of IL-2 receptor | 第15-16页 |
| 1.3 The signal pathway of IL-2 | 第16-20页 |
| 1.3.1 JAK/ STAT5 signal pathway | 第17页 |
| 1.3.2 RAS/ MAPK signal pathway | 第17-18页 |
| 1.3.3 PI3K/ AKT signal pathways | 第18-20页 |
| 1.4 The clinical application of IL-2 | 第20-22页 |
| 1.4.1 The clinical application of low dosage IL-2 | 第20-21页 |
| 1.4.2 The clinical application of high dosage IL-2 | 第21-22页 |
| 1.5 Summary | 第22-24页 |
| CHAPTER2 Construction and purification of SF-H2 and SF-H9RETR | 第24-56页 |
| 2.1 Introduction | 第24页 |
| 2.2 Materials and Instruments | 第24-34页 |
| 2.2.1 Materials | 第25页 |
| 2.2.2 Reagents | 第25-28页 |
| 2.2.3 Solution preparation | 第28-32页 |
| 2.2.4 Expriment instruments | 第32-34页 |
| 2.3 Method | 第34-46页 |
| 2.3.1 Construction of Recombinant Plasmids SF- H2 and SF- H9RETR | 第34-37页 |
| 2.3.2 Screening of recombinant plasmid SF- H2/ SF- H9RETR | 第37-40页 |
| 2.3.3 Screen SF- H2 and SF- H9RETR high expression strains | 第40-42页 |
| 2.3.4 Purification of SF- H2/ SF- H9RETR | 第42-44页 |
| 2.3.5 SF- H2 and SF- H9RETR protein identification | 第44-46页 |
| 2.4 Result | 第46-54页 |
| 2.4.1 Construction of Recombinant Plasmids SF- H2 and SF- H9RETR | 第46-48页 |
| 2.4.2 Screening of recombinant plasmid SF- H2/ SF- H9RETR | 第48-50页 |
| 2.4.3 Screen SF- H2 and SF- H9RETR high expression strains | 第50-52页 |
| 2.4.4 The protein purification of SF- H2/ SF- H9RETR | 第52页 |
| 2.4.5 SF- H2 and SF- H9RETR protein identification | 第52-54页 |
| 2.5 Summary | 第54-56页 |
| CHAPTER3 Explore the similarities and differences between SF-H2 and SF-H9RETR signaling pathways | 第56-68页 |
| 3.1 Introduction | 第56页 |
| 3.2 Materials and Instruments | 第56-59页 |
| 3.2.1 Materials | 第56页 |
| 3.2.2 Reagents | 第56-58页 |
| 3.2.3 Solution preparation | 第58页 |
| 3.2.4 Expriment instruments | 第58-59页 |
| 3.3 Method | 第59-63页 |
| 3.3.1 Endotoxin removal in SF-H2 and SF-H9RETR | 第59页 |
| 3.3.2 Cell culture methods | 第59-60页 |
| 3.3.3 Detection of the activity of SF-H2 and SF-H9RETR by MTT | 第60页 |
| 3.3.4 Western blot analysis of the similarities and differences between SF-H2 and SF-H9RETR signaling pathways | 第60-61页 |
| 3.3.5 Mass spectrometry analysis of the similarities and differences between SF-H2 and SF-H9RETR signaling pathways | 第61-63页 |
| 3.4 Results | 第63-67页 |
| 3.4.1 Detection of the activity of SF-H2 and SF-H9RETR by MTT | 第63-65页 |
| 3.4.2 Western blot analysis of the similarities and differences between SF-H2 and SF-H9RETR signaling pathways | 第65-66页 |
| 3.4.3 Mass spectrometry analysis of the similarities and differences between SF-H2 and SF-H9RETR signaling pathways | 第66-67页 |
| 3.5 Summary | 第67-68页 |
| CHAPTER4 Construction and purification of DF-H2 and DF-H9RETR | 第68-86页 |
| 4.1 Introduction | 第68页 |
| 4.2 Materials and Instruments | 第68-69页 |
| 4.2.1 Materials | 第68页 |
| 4.2.2 Reagents | 第68页 |
| 4.2.3 Solution preparation | 第68-69页 |
| 4.2.4 Instruments | 第69页 |
| 4.3 Method | 第69-77页 |
| 4.3.1 Construction of Recombinant Plasmids DF- H2C and DF- H9CRE.. | 第69-73页 |
| 4.3.2 Screening of recombinant plasmid DF- H2C/ DF- H9CRE | 第73-75页 |
| 4.3.3 Screen DF- H2c and DF- H9CRE high expression strains | 第75-76页 |
| 4.3.4 The protein purification of DF- H2C and DF- H9CRE | 第76-77页 |
| 4.3.5 DF- H2C and DF- H9CRE protein identification | 第77页 |
| 4.4.Results | 第77-84页 |
| 4.4.1 Construction of Recombinant Plasmids DF- H2C and DF- H9CRE.. | 第77-78页 |
| 4.4.2 Screening of recombinant plasmid DF-H2C and DF-H9CRE | 第78-81页 |
| 4.4.3 Screen DF- H2C and DF- H9CRE high expression strains | 第81-82页 |
| 4.4.4 The protein purification of DF- H2C/ DF- H9CRE | 第82-83页 |
| 4.4.5 DF- H2C and DF- H9CRE protein identification | 第83-84页 |
| 4.5 Summary | 第84-86页 |
| CHAPTER5 Explore the similarities and differences between DF-H2C and DF-H9CRE signaling pathways | 第86-96页 |
| 5.1 Introduction | 第86页 |
| 5.2 Materials and Instruments | 第86-87页 |
| 5.2.1 Materials | 第86页 |
| 5.2.2 Reagents | 第86页 |
| 5.2.3 Solution preparation | 第86页 |
| 5.2.4 Expriment instruments | 第86-87页 |
| 5.3 Method | 第87-88页 |
| 5.3.1 Endotoxin removal in DF-H2C and DF-H9CRE | 第87页 |
| 5.3.2 Detection of the activity of DF-H2C and DF-H9CRE by MTT | 第87页 |
| 5.3.3 Western blot analysis of the similarities and differences between DF-H2C and DF-H9CRE signaling pathways | 第87-88页 |
| 5.3.4 Mass spectrometry analysis of the similarities and differences between DF-H2C and DF-H9CRE signaling pathways | 第88页 |
| 5.4 Results | 第88-93页 |
| 5.4.1 Detection of the activity of DF-H2C and DF-H9CRE by MTT | 第88-90页 |
| 5.4.2 Western blot analysis of the similarities and differences between DF-H2C and DF-H9CRE signaling pathways | 第90-93页 |
| 5.4.3 Mass spectrometry analysis of the similarities and differences between DF-H2C and DF-H9CRE signaling pathways | 第93页 |
| 5.5 Summary | 第93-96页 |
| CHAPTER6 Discussion | 第96-98页 |
| Reference | 第98-104页 |
| Publications and participation in scientific research | 第104-106页 |
| Acknowledgements | 第106页 |
