| 附件 | 第6-7页 |
| 摘要 | 第7-8页 |
| Abstract | 第8-9页 |
| LIST OF ABBREVIATIONS | 第13-20页 |
| 1 Chapter 1 Introduction | 第20-29页 |
| 1.1 Preamble | 第20-21页 |
| 1.2 Natural variation, flowering time, and maturity trait in soybean | 第21-22页 |
| 1.3 Photoperiodism phenomenon- remarkable findings in plant science | 第22-23页 |
| 1.4 Regulatory networks and genes influencing flowering time in plants | 第23-27页 |
| 1.5 Establishment of soybean growth season | 第27-28页 |
| 1.6 The purpose and significance of this study | 第28-29页 |
| 2 Chapter 2 GmCOL Genes in Maturity and Flowering Time | 第29-42页 |
| 2.1 Photoperiod and relevant genes that influencing maturity and flowering | 第29-30页 |
| 2.2 CONSTANS and CONSTANS-Like genes | 第30-31页 |
| 2.3 Functional and evolutionary role of COL family gene in soybean | 第31-35页 |
| 2.4 Flowering time regulated by day length | 第35-36页 |
| 2.5 Co-relation between maturity, flowering and CONSTANS genes | 第36-37页 |
| 2.6 Regulatory role of COL family genes in photoperiod responsive in flowering in different species | 第37-38页 |
| 2.7 Identification and selection of determinate growth habit MG cultivars | 第38-42页 |
| 3 Chapter 3 Materials and Methods | 第42-51页 |
| 3.1 Phenotype investigation of soybean in different maturity groups | 第42-46页 |
| 3.1.1 Experimental materials | 第42-45页 |
| 3.1.2 Methods | 第45页 |
| 3.1.3 Phenotypic evaluation | 第45页 |
| 3.1.4 Growth traits | 第45页 |
| 3.1.5 Phenotypic evaluation of stem growth habits | 第45-46页 |
| 3.2 Genetic Study | 第46-51页 |
| 3.2.1 Plant materials | 第46页 |
| 3.2.2 Genetic materials | 第46页 |
| 3.2.3 Gene identity of 21 GmCOL family genes | 第46-47页 |
| 3.2.4 Genomic DNA extraction | 第47页 |
| 3.2.5 Data mining and primer design | 第47-49页 |
| 3.2.6 PCR amplification and purification | 第49-50页 |
| 3.2.7 Sequencing and sequence data analysis | 第50-51页 |
| 4 Chapter 4 Results and Analysis | 第51-129页 |
| 4.1 Analysis of soybean varieties in different growth stages | 第51-68页 |
| 4.1.1 Investigation results of growth period traits | 第51-58页 |
| 4.1.2 Association of flowering and maturity dates of soybean shown divergence | 第58-60页 |
| 4.1.3 Clustering analysis | 第60-62页 |
| 4.1.4 Analysis of soybean maturity group varieties growth period | 第62-64页 |
| 4.1.5 Soybean varieties matured differently with different beginning bloom days | 第64-68页 |
| 4.2 Bioinformatics analysis | 第68-70页 |
| 4.2.1 Construction and analysis of phylogram of COL genes in soybean | 第68-69页 |
| 4.2.2 Analysis and association of soybean determinacy | 第69-70页 |
| 4.3 Sequencing results: Analysis of COL genes in soybean varieties with different growth stages | 第70-129页 |
| 4.3.1 Gene sequencing results and genotype analysis | 第70-80页 |
| 4.3.2 Genotype distribution of GmCOL family genes in soybean cultivars | 第80-89页 |
| 4.3.3 Genotype number and genotype frequency of GmCOL genes | 第89-91页 |
| 4.3.4 GmCOL genes maturity groups and genotype distribution | 第91-94页 |
| 4.3.5 Statistical analysis of genotypes and phenotypes | 第94-99页 |
| 4.3.6 Association analysis GmCOL genotypes and pairwise comparison effect | 第99-104页 |
| 4.3.7 Soybean COL gene family exhibited different linkage disequilibrium | 第104-106页 |
| 4.3.8 Haplotype analysis of 21 soybean GmCOL family genes | 第106-126页 |
| 4.3.9 Haplotypes and natural variation of flowering time of GmCOL family genes… | 第126-129页 |
| 5 Chapter 5 Discussion | 第129-134页 |
| 5.1 Selected soybean cultivars from different maturity groups show diversity in flowering time and maturity | 第129-130页 |
| 5.2 Natural variation, growth period group system and genotypes at the GmCOL loci of accessions | 第130-132页 |
| 5.3 Conservation and divergence of TFL1 homologs in soybean and other species | 第132页 |
| 5.4 Polymorphism of GmCOL family genes – association to flowering time and maturity… | 第132-134页 |
| 6 Chapter 6 Summery and Conclusion | 第134-136页 |
| 7 Chapter 7 References | 第136-150页 |
| Appendixes | 第150-174页 |
| Resume | 第174-175页 |
| Acknowledgements | 第175页 |
