| DEDICATION | 第4-10页 |
| SUMMARY | 第10-12页 |
| LIST OF ABBREVIATIONS | 第13-15页 |
| PART 1 THE REVIEW OF THE LITERATURE | 第15-29页 |
| CHAPTER ONE THE REVIEW OF THE LITERATURE | 第17-29页 |
| 1.1 Stress and heat shock response | 第17-18页 |
| 1.2 Heat shock proteins and their functions | 第18-21页 |
| 1.3 Heat shock protein regulation | 第21-22页 |
| 1.4 Heat shock protein expression in heat stress treatment and tissue damage | 第22页 |
| 1.5 Heat shock protein 47 (Hsp47) | 第22-25页 |
| 1.5.1 The regulation of Hsp47 | 第22-23页 |
| 1.5.2 Localization of Hsp47 | 第23页 |
| 1.5.3 The functions of Hsp47 | 第23-25页 |
| 1.6 Hsp47 in pathological and other conditions | 第25-27页 |
| 1.6.1 Hsp47 in fibrotic conditions | 第25-26页 |
| 1.6.2 Hsp47 in tissue transplantation | 第26页 |
| 1.6.3 Hsp47 in wound healing | 第26-27页 |
| 1.6.4 Hsp47 during development | 第27页 |
| 1.6.5 Hsp47 in cancer | 第27页 |
| 1.7 Objectives of the Thesis | 第27-29页 |
| PART 2 EXPERIMENTAL STUDIES | 第29-87页 |
| CHAPTER TWO Expression and localization of Hsp47 in heat-stressed H9c2 cells: correlationwith myocardial cells damage in vitro | 第31-45页 |
| 2.1 Introduction | 第31-33页 |
| 2.2 Materials and methods | 第33-35页 |
| 2.2.1 Cell culture and heat shock treatment | 第33页 |
| 2.2.2 Cardiac marker enzymes activity assay | 第33-34页 |
| 2.2.3 Cytopathology | 第34-35页 |
| 2.2.4 Immunofluorescence assay (IFA) | 第35页 |
| 2.2.5 Statistical analysis | 第35页 |
| 2.3 Results | 第35-39页 |
| 2.3.1 Detection of enzyme levels in H9c2 cell supernatant after heat treatment | 第35-36页 |
| 2.3.2 Cytopathological changes in heat-stressed H9c2 cells | 第36-37页 |
| 2.3.3 Immunofluorescence staining of heat-stressed H9c2 cells in vitro | 第37-39页 |
| 2.4 Discussion | 第39-41页 |
| REFERENCES | 第41-45页 |
| CHAPTER THREE Expression and localization of Hsp47 in heat-stressed rat hearts: correlationwith heart tissue damage in vivo | 第45-59页 |
| 3.1 Introduction | 第45-47页 |
| 3.2 Materials and methods | 第47-49页 |
| 3.2.1 Experimental design and heat stress treatment of rats | 第47页 |
| 3.2.2 Detection of myocardial cell damage related enzymes | 第47页 |
| 3.2.3 Histopathological observations | 第47-48页 |
| 3.2.4 Immunohistochemistry (IHC) | 第48页 |
| 3.2.5 Statistical analysis | 第48-49页 |
| 3.3 Results | 第49-52页 |
| 3.3.1 Clinical symptoms of heat-stressed animals | 第49页 |
| 3.3.2 Detection of enzyme levels in rat serum after heat treatment | 第49页 |
| 3.3.3 Histopathological changes in heat-stressed myocardial cells in vivo | 第49-50页 |
| 3.3.4 Immunohistochemical staining of heat-stressed myocardial cells in vivo | 第50-52页 |
| 3.4 Discussion | 第52-55页 |
| REFERENCES | 第55-59页 |
| CHAPTER FOUR Expression of Hsp47 and its corresponding mRNA in heat-stressed ratsmyocardial cells in vitro and in vivo | 第59-75页 |
| 4.1 Introduction | 第59-61页 |
| 4.2 Materials and methods | 第61-66页 |
| 4.2.1 Cell culture and heat stress treatment of the myocardial cells in vitro | 第61-62页 |
| 4.2.2 Experimental design and heat stress treatment of rats in vivo | 第62页 |
| 4.2.3 Detection of hsp47 mRNA by quantitative real-time PCR (qRT-PCR) | 第62-64页 |
| 4.2.4 Immuno-blotting | 第64-66页 |
| 4.2.5 Statistical analysis | 第66页 |
| 4.3 Results | 第66-69页 |
| 4.3.1 Transcriptional levels of hsp47 mRNA in heat-stressed myocardial cells in vitro and in vivo | 第66页 |
| 4.3.2 Variations in expression of Hsp47 in heat-stressed myocardial cells in vitro and in vivo | 第66-69页 |
| 4.4 Discussion | 第69-71页 |
| REFERENCES | 第71-75页 |
| CHAPTER FIVE Association of Hsp47 expression with HSF-1 in myocardial cells of rats in vitroand in vivo | 第75-87页 |
| 5.1 Introduction | 第75-79页 |
| 5.2 Materials and methods | 第79-80页 |
| 5.2.1 Experimental design and heat stress treatment of rats | 第79页 |
| 5.2.2 Cell culture and heat stress treatment of the H_9C_2 myocardial cells | 第79页 |
| 5.2.3 ELISA detection of HSF-1 in vivo | 第79-80页 |
| 5.2.4 ELISA detection of HSF-1 in vitro | 第80页 |
| 5.2.5 Statistical analysis | 第80页 |
| 5.3 Results | 第80-82页 |
| 5.3.1 ELISA detection of HSF-1 in vivo | 第80-81页 |
| 5.3.2 ELISA detection of HSF-1 in vitro | 第81-82页 |
| 5.4 Discussion | 第82-84页 |
| REFERENCES | 第84-87页 |
| CONCLUSION | 第87-89页 |
| INNOVATION | 第89-91页 |
| PUBLICATIONS | 第91-93页 |
| ACKNOWLEDGEMENTS | 第93页 |
