| 摘要 | 第3-4页 |
| Abstract | 第4-5页 |
| List of abbreviations | 第9-11页 |
| Introduction | 第11-30页 |
| 1 Postpartum endometritis | 第11-16页 |
| 1.1 Causes of postpartum endometritis | 第12页 |
| 1.2 Symptoms of partum endometritis | 第12-13页 |
| 1.3 Diagnosis of endometritis and subclinical endometritis | 第13页 |
| 1.4 Treatment of endometritis in dairy cows | 第13-14页 |
| 1.5 The postpartum uterus | 第14-15页 |
| 1.6 Local immunity of the endometrium | 第15-16页 |
| 1.7 Changes in the uterus during endometritis | 第16页 |
| 2 Cortisol | 第16-23页 |
| 2.1 Glucocorticoids | 第16-18页 |
| 2.2 Mechanism of action | 第18-19页 |
| 2.3 Glucocorticoids and inflammation | 第19页 |
| 2.4 Anti-Inflammatory Effects of Glucocorticoids | 第19-20页 |
| 2.5 Pro-Inflammatory Effects of Glucocorticoids | 第20-21页 |
| 2.6 Glucocorticoids and recovery | 第21页 |
| 2.7 Glucocorticoids during pregnancy | 第21-23页 |
| 3 References | 第23-30页 |
| Experiment 1:The effect of cortisol on the physical and blood parameters of goats withendometritis induced by E.coli | 第30-43页 |
| 1 Materials | 第30-32页 |
| 1.1 Animals | 第30页 |
| 1.2 Instruments | 第30-31页 |
| 1.3 Reagents | 第31页 |
| 1.4 Prepared reagents | 第31-32页 |
| 2 Experimental method | 第32-34页 |
| 2.1 Preparation of bacteria | 第32页 |
| 2.2 Preparation of animals and groups | 第32页 |
| 2.3 Inoculation of bacteria | 第32-33页 |
| 2.4 Clinical inspection | 第33-34页 |
| 2.5 Uterine discharge cytology | 第34页 |
| 3 Experimental results | 第34-39页 |
| 3.1 Clinical inspection | 第34-38页 |
| 3.2 Uterine cytology examination results | 第38-39页 |
| 4 Discussion | 第39-41页 |
| 5 References | 第41-43页 |
| Experiment 2:The effect of cortisol on the pathological tissue structure of goats with endometritisinduced by E. coli | 第43-51页 |
| 1 Materials | 第43-45页 |
| 1.1 Animals and experimental groups | 第43页 |
| 1.2 Instruments | 第43页 |
| 1.3 Reagents | 第43-44页 |
| 1.4 Prepared reagents | 第44-45页 |
| 2 Experimental methods | 第45-47页 |
| 2.1 Collection and fixation of samples | 第45页 |
| 2.2 Preparation of paraffin sections | 第45-47页 |
| 3 Results | 第47页 |
| 4 Discussion | 第47-49页 |
| 5 References | 第49-51页 |
| Experiment 3:The effect of cortisol on mRNA expression of TLR4 and other inflammatorycytokines in endometrial tissue of the goat with endometritis induced by E. coli | 第51-65页 |
| 1 Materials | 第51-53页 |
| 1.1 Animals and experimental groups | 第51页 |
| 1.2 Instruments | 第51页 |
| 1.3 Reagents | 第51-52页 |
| 1.4 Prepared reagents | 第52-53页 |
| 2 Methods | 第53-58页 |
| 2.1 Collection and preparation of sample | 第53页 |
| 2.2 Real-time fluorescence quantitative PCR | 第53-58页 |
| 3 Results | 第58-61页 |
| 3.1 The effect of elevated cortisol levels on TLR4 mRNA expression in the endometrialtissue of E. coli induced endometritis in goats | 第58页 |
| 3.2 The effect of elevated cortisol levels on mRNA expression of inflammatory factors inthe endometrial tissue of E. coli induced endometritis in goats | 第58-60页 |
| 3.3 The effect of elevated cortisol levels on mRNA expression of recovery related genes inthe endometrial tissue of E. coli induced endometritis in goats | 第60-61页 |
| 4 Discussion | 第61-63页 |
| 5 References | 第63-65页 |
| Conclusion | 第65-66页 |
| Acknowledgement | 第66-67页 |
