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陆地棉杂种优势的全基因组关联分析

摘要第6-7页
Abstract第7页
DEDICATIONS第8-17页
Abbreviations第17-19页
CHAPTER 1: INTRODUCTION第19-48页
    1.1 Plant breeding in the modern era第19-20页
    1.2 Cotton origin and domestication第20-21页
    1.3 Current status of cotton production第21-22页
    1.4 Spectrum of genetic diversity第22-24页
    1.5 Genetic diversity in the cultivated cottons第24-27页
    1.6 Hybrid vigor第27-33页
        1.6.1 Dominance model of heterosis第27-28页
        1.6.2 Overdominance model of heterosis第28-30页
        1.6.3 Prediction of heterosis第30-31页
        1.6.4 Phenotypic data-based prediction of heterosis第31-32页
        1.6.5 Molecular marker-based prediction of heterosis第32-33页
    1.7 Cotton as a model crop for heterosis第33-36页
    1.8 Significance of F1 for commercial production of cotton第36-37页
    1.9 Cotton research updates and advances第37-42页
        1.9.1 Advanced mapping and sequencing technologies第37-38页
        1.9.2 Single nucleotide polymorphisms (SNPs)第38-39页
        1.9.3 RAD Sequencing第39-40页
        1.9.4 Successful achievements of whole genomes assembling第40-41页
        1.9.5 Transcriptomics and gossypomics第41-42页
    1.10 Justifications for approaching GWAS第42-46页
    Objectives of Research第46-48页
CHAPTER 2: MATERIALS AND METHODS.第48-64页
    2.1 Association mapping panel construction第48页
    2.2 Mating design第48页
    2.3 Experimental locations第48-49页
    2.4 Experimental design and procedures第49-50页
    2.5 Data collection of phenotypic traits第50-54页
        2.5.1 Plant height (cm)第51页
        2.5.2 Bolls per plant (no.)第51页
        2.5.3 Boll weight (g)第51页
        2.5.4 Lint percentage (%)第51页
        2.5.5 Fiber length (mm)第51-52页
        2.5.6 Micronaire value (μg inch-1)第52页
        2.5.7 Fiber strength (g tex-1)第52页
        2.5.8 Fiber elongation (%)第52-53页
        2.5.9 Length uniformity index (%)第53页
        2.5.10 Fiber uniformity ratio (%)第53-54页
    2.6 Microsatellites genotyping第54-56页
        2.6.1 DNA quantification and qualification第54-55页
        2.6.2 Polymerase chain reaction and electrophoresis第55-56页
    2.7 SNP genotyping第56页
        2.7.1 DNA extraction第56页
        2.7.2 Sample preparation for RAD sequencing第56页
    2.8 Phenotypic data analysis第56-59页
        2.8.1 Principal component analysis第57-59页
    2.9 Genotypic data analysis of microsatellites第59-61页
        2.9.1 Population structure第59-60页
        2.9.2 Linkage disequilibrium第60页
        2.9.3 Association analysis and superior allele identification第60-61页
    2.10 Genotypic data analysis of SNPs第61-64页
        2.10.1 Alignment and genotyping第61-62页
        2.10.2 Population genetic analysis and GWAS第62页
        2.10.3 Haplotype block analysis第62-63页
        2.10.4 Candidate gene finding第63-64页
CHAPTER 3: RESULTS第64-118页
    3.1 Phenotypic data analysis第64-73页
        3.1.1 Summary statistics of phenotypic data第64页
        3.1.2 Correlation (r) matrix (Pearson (n))第64-65页
        3.1.3 Principal component analysis第65-73页
    3.2 Genotypic data analysis of microsatellites第73-86页
        3.2.1 Population structure第73页
        3.2.2 Linkage disequilibrium analysis第73-76页
        3.2.3 Association studies between microsatellites and phenotypic traits第76页
        3.2.4 Traits associated with microsatellites第76-84页
        3.2.5 Discovery of favorable alleles第84-86页
    3.3 Genotypic data analysis of SNPs第86-118页
        3.3.1 Population structure第86-88页
        3.3.2 Genetic variation based on SNPs第88-91页
        3.3.3 Common SNPs among F1 sets第91页
        3.3.4 Common QTNs among variables第91-93页
            3.3.4.1 h QTNs with additive effects第92页
            3.3.4.2 QTNs with dominant effects第92页
            3.3.4.3 h QTNs with additive × dominant effects第92页
            3.3.4.4 QTN with additive × additive effect第92-93页
        3.3.5 Significant associations for pleiotropic and unique SNPs第93-99页
        3.3.6 Mining of associated key SNPs第99-101页
        3.3.7 Identified hotspot genomic regions第101-102页
        3.3.8 Identification of candidate genes associated with SNPs第102-118页
CHAPTER 4: DISCUSSIONS第118-123页
    4.1 Breeding design and phenotyping第118-120页
    4.2 Heterosis related loci identified with microsatellites and SNPs第120-121页
    4.3 Usefulness of germplasm used第121-123页
CONCLUSIONS第123-124页
References (参考文献)第124-143页
Acknowledgements第143-145页
Curriculum Vitae (个人简介)第145-147页
Appendices第147-192页

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