| Abstract | 第1-7页 |
| 摘要 | 第7-9页 |
| TABLE OF CONTENTS | 第9-13页 |
| LIST OF SCHEMES | 第13-14页 |
| LIST OF FIGURES | 第14-16页 |
| LIST OF TABLES | 第16-17页 |
| LIST OF SYMBOLS AND ABBREVIATIONS | 第17-19页 |
| CHAPTER 1 INTRODUCTION | 第19-32页 |
| ·Recent depvelopment of nanomaterials used in DNA biosensors | 第19-20页 |
| ·Emerging nanomaterials used in this thesis | 第20-31页 |
| ·Gold nanoparticles | 第20-23页 |
| ·Introduction | 第20页 |
| ·Properties of gold nanoparticles | 第20-22页 |
| ·Applications of gold nanoparticles | 第22-23页 |
| a. Application of nanoparticles as substrates for DNA attachment | 第22页 |
| b. Application of nanoparticles for signal amplification | 第22-23页 |
| ·Silver nanoclusters | 第23-27页 |
| ·Introduction | 第23-24页 |
| ·Properties of silver nanoclusters | 第24-26页 |
| a. Optical properties of fluorescent silver nanoclusters | 第24页 |
| b. Oligonucleotide-stabilized silver nanocluster fluorophores | 第24-26页 |
| ·Applications of silver nanoclusters to analytical detection | 第26-27页 |
| a. Cell staining | 第26页 |
| b. Detection of metal ions and small biomolecules | 第26-27页 |
| c. Detection of proteins | 第27页 |
| ·Graphene oxide | 第27-31页 |
| ·Introduction | 第27-28页 |
| ·Properties of graphene oxide | 第28-29页 |
| ·Applications of graphene oxide in biosensors | 第29-31页 |
| a. Biosensors by fluorescence quenching in GO | 第29-30页 |
| b. Electrochemical biosensors | 第30-31页 |
| ·Outline of the thesis | 第31-32页 |
| CHAPTER 2 USING PROPERTIES OF GOLD NANOPARTICLES AND SYBR GREEN I FOR DETECTION URACIL-DNA GLYCOSYLASE ACTIVITY | 第32-57页 |
| ·Introduction | 第32-36页 |
| ·Enzyme-catalyzed assembly of gold nanoparticles for visualized screening of DNA base excision repair | 第36-46页 |
| ·Experimental | 第36-38页 |
| ·Apparatuses and reagents | 第36-37页 |
| a. Apparatuses | 第36页 |
| b. Reagents | 第36-37页 |
| ·Preparation of DNA probe-decorated AuNPs | 第37页 |
| ·Activity assay of UDG | 第37-38页 |
| ·Results and discussion | 第38-46页 |
| ·Probe design and analytical principle | 第38-39页 |
| ·Optimization of assay conditions | 第39-41页 |
| ·Assay of UDG activity | 第41-46页 |
| a. Enzyme-catalyzed assembly of AuNPs for visualized activity screening of UDG | 第41-43页 |
| b. Dynamic light scattering characterization of the biosensing strategy for UDG assay | 第43-44页 |
| c. Quantitative activity screening of UDG using the developed biosensing strategy | 第44-46页 |
| ·Performance and reliability of the SYBR Green I based assay for the uracil-DNA glycosylase activity | 第46-56页 |
| ·Experimental | 第46-48页 |
| ·Apparatuses and reagents | 第46页 |
| ·Preparation reagents | 第46-47页 |
| ·Optimization of assay conditions | 第47页 |
| a. Real-time monitoring of uracil removal | 第47页 |
| b. DNA hairpin probe concentration | 第47页 |
| c. Optimization of Endo IV concentration | 第47页 |
| ·Assay for UDG activity | 第47-48页 |
| ·Results and discussion | 第48-56页 |
| ·Probe design and analytical principle | 第48-49页 |
| ·Optimization of assay conditions | 第49-53页 |
| a. Real-time monitoring of uracil removal | 第49-50页 |
| b. DNA hairpin probe concentration | 第50-51页 |
| c. Optimal of Endo IV concentration | 第51-53页 |
| ·Assay for UDG activity | 第53-56页 |
| a. Control UDG activity | 第53-54页 |
| b. Assay for UDG activity | 第54-56页 |
| ·Conclusion | 第56-57页 |
| CHAPTER 3 A LABEL-FREE FLUORESCENT BASE ON DNA TEMPLATE SILVER NANOCLUSTERS FOR DETECTION OF O6-METHYLGUANINE-DNA METHYLTRANFERASE ACTIVITY | 第57-69页 |
| ·Introduction | 第57-59页 |
| ·Experimental | 第59-61页 |
| ·Apparatuses and reagents | 第59页 |
| ·Apparatuses | 第59页 |
| ·Reagents | 第59页 |
| ·Preparation of silver nanoclusters on DNA | 第59-60页 |
| ·Fluorescence measurements | 第60页 |
| ·Activity assay of MGMT | 第60页 |
| ·Gel electrophoresis analysis of MGMT activity | 第60-61页 |
| ·Results and discussion | 第61-68页 |
| ·Probe design and analytical principle | 第61-62页 |
| ·Typical characteristics of the developed biosensor in MGMT activity assay | 第62-63页 |
| ·Optimization of DNA-AgNCs synthesis conditions | 第63-64页 |
| ·Optimization of factors affecting MGMT activity | 第64-66页 |
| ·Electrophoresis characterization of the biosensing strategy for MGMT assay | 第66-67页 |
| ·Quantitative activity screening of MGMT using the developed biosensing strategy | 第67-68页 |
| ·Conclusion | 第68-69页 |
| CHAPTER 4 FLUORESCENCE DETECTION OF MELAMINE BASE ON FUNCTIONALIZED GRAPHENE OXIDE | 第69-82页 |
| ·Introduction | 第69-72页 |
| ·Experimental | 第72-75页 |
| ·Apparatuses and reagents | 第72-73页 |
| ·Synthesis of graphene oxide | 第73页 |
| ·Verifying the specificity of the sensor | 第73页 |
| ·Optimization of assay conditions | 第73-74页 |
| ·Optimization of SG concentration | 第73-74页 |
| ·Optimization of GO concentration | 第74页 |
| ·Optimization of T_(55) concentration | 第74页 |
| ·Detection of melamine | 第74-75页 |
| ·Results and discussion | 第75-81页 |
| ·Probe design and analytical principle | 第75-76页 |
| ·Signal amplification of the sensing system | 第76-77页 |
| ·Optimization of analytical conditions | 第77-79页 |
| ·Optimization of SG concentration | 第77-78页 |
| ·Optimization of GO concentration | 第78-79页 |
| ·Optimization of T_(55) concentration | 第79页 |
| ·Detection of melamine | 第79-81页 |
| ·Conclusion | 第81-82页 |
| CONCLUSION AND FUTURE WORK | 第82-83页 |
| REFERENCES | 第83-97页 |
| RESEARCH PUBLICATIONS | 第97-98页 |
| ACKNOWLEDGEMENTS | 第98页 |
