| 博士学位论文评阅人、答辩委员会签名表 | 第5-6页 |
| DEDICATIONS | 第6-7页 |
| 摘要 | 第7-8页 |
| ABSTRACT | 第8-9页 |
| ABBREVIATION | 第12-18页 |
| 第一章 CHAPTER 1 LITERATURE REVIEW | 第18-55页 |
| 1.1 EXPANSIN OVERVIEW | 第18-20页 |
| 1.2 NOMENCLATURE OF EXPANSIN GENE FAMILY | 第20-22页 |
| 1.2.1 Expansin and expansin-like genes and their proteins | 第20-21页 |
| 1.2.2 General rules of expansin nomenclature | 第21-22页 |
| 1.3 EXPANSINS AND PLANT GROWTH AND DEVELOPMENT | 第22-27页 |
| 1.4 EFFECTS ON SPECIFIC DEVELOPMENTAL STAGES FROM GERMINATION TO FRUITING | 第27-44页 |
| 1.4.1 Effects on Germination | 第27-28页 |
| 1.4.2 Effects on root development and growth | 第28-30页 |
| 1.4.3 Effects on leaf initiation and leaf growth | 第30-31页 |
| 1.4.4 Effects on stomata opening and closing | 第31-32页 |
| 1.4.5 Effects on stem elongation | 第32-34页 |
| 1.4.6 Expansin and reproduction | 第34-37页 |
| 1.4.7 Effects on fruit ripening and softening | 第37-38页 |
| 1.4.8 Effects on crop yield | 第38-40页 |
| 1.4.9 Effects on biotic and abiotic stress tolerance | 第40-44页 |
| 1.4.10 Expansins and tobacco | 第44页 |
| 1.5 CONCLUSION | 第44-46页 |
| REFERENCES | 第46-55页 |
| 第二章 CHAPTER 2 GENOME WIDE ANALYSIS OF TOBACCO EXPANSIN GENEFAMILY | 第55-86页 |
| 2.1 INTRODUCTION | 第55-57页 |
| 2.2 MATERIAL AND METHODS | 第57-60页 |
| 2.2.1 Identification and sequence analysis of the tobacco expansin gene | 第57-59页 |
| 2.2.2 Expression analysis of tobacco expansin genes | 第59页 |
| 2.2.3 Plant materials | 第59-60页 |
| 2.2.4 RNA isolation and q RT-PCR analysis | 第60页 |
| 2.3 RESULTS | 第60-77页 |
| 2.3.1 Identification of expansin gene family in tobacco | 第60-64页 |
| 2.3.2 Phylogenetic and structural analysis of tobacco expansin genes | 第64-66页 |
| 2.3.3 Go analysis | 第66-68页 |
| 2.3.4 Cis-acting elements detection in the promoter regions of Nt EXPs | 第68-72页 |
| 2.3.5 Delineating spatial-temporal patterns of expansin genes expression | 第72-74页 |
| 2.3.6 Expression analysis of Nt EXPs tobacco tissues with q RT-PCR | 第74-77页 |
| 2.4 DISCUSSION | 第77-81页 |
| 2.5 CONCLUSION | 第81-82页 |
| REFERENCES | 第82-86页 |
| 第三章 CHAPTER 3 OVEREXP RESSION OF AN EXPANSIN GENE MODULATES PLANTS GROWTH AND DEVELOPMENT AND CONFERS STRESS TOLERANCE IN TOBACCO | 第86-119页 |
| 3.1 INTRODUCTION | 第86-88页 |
| 3.2 MATERIALS AND METHODS | 第88-92页 |
| 3.2.1 Selection of the expansin genes | 第88页 |
| 3.2.2 Cis-acting regulatory elements | 第88页 |
| 3.2.3 Plant growth conditions | 第88-89页 |
| 3.2.4 Electroporation | 第89-90页 |
| 3.2.5 Agrobacterium mediated leaf disc transformation | 第90-91页 |
| 3.2.6 Plant genomic DNA extraction | 第91页 |
| 3.2.7 Polymerase chain reaction (PCR) | 第91-92页 |
| 3.2.8 Semi-quantitative reverse transcriptase PCR (RT-PCR) | 第92页 |
| 3.2.9 Agarose gel electrophoresis | 第92页 |
| 3.3 EVALUATION OF T3 TRANSGENIC PLANTS | 第92-95页 |
| 3.3.1 Measurement of morphological and physiological properties of transgenic plants | 第92-93页 |
| 3.3.2 Micro-techniques and microscopy | 第93页 |
| 3.3.3 Effect of overexpressing NtEXPA11 on tobacco polysaccharides | 第93页 |
| 3.3.4 Effect of overexpressing NtEXPA11 on abiotic stress tolerance | 第93-95页 |
| 3.3.4.1 Salt stress tolerance | 第94页 |
| 3.3.4.2 Drought tolerance | 第94页 |
| 3.3.4.3 Histochemical detection of H2O2 | 第94-95页 |
| 3.4 STATISTICAL ANALYSIS | 第95页 |
| 3.5 RESULTS | 第95-110页 |
| 3.5.1 Selecting the expansin genes | 第95-96页 |
| 3.5.2 The deduced cis-regulating elements in the promoter region of NtEXPA11 | 第96-98页 |
| 3.5.3 Global expression pattern of NtEXPA11 in tobacco | 第98-99页 |
| 3.5.4 Vegetative growth and development was enhanced in NtEXPA11-ox plants | 第99页 |
| 3.5.5 Root growth and development was enhanced in NtEXPA11-ox plants | 第99-101页 |
| 3.5.6 NtEXPA11 overexpression improved stem growth and development | 第101-103页 |
| 3.5.7 Accumulation of NtEXPA11 enlarged cell size | 第103-105页 |
| 3.5.8 Overexpression of NtEXPA11 modulated leaf growth and development | 第105-106页 |
| 3.5.9 Overexpression of NtEXPA11 modulated tobacco polysaccharides | 第106页 |
| 3.5.10 Overexpression of NtEXPA11 increased abiotic stress tolerance | 第106-110页 |
| 3.6 DISCUSSION | 第110-115页 |
| 3.6.1 Overexpression of NtEXPA11 modulated plant growth and development | 第110-113页 |
| 3.6.2 Overexpression of NtEXPA11 enhanced salt and drought tolerance | 第113-115页 |
| 3.7 CONCLUSION | 第115页 |
| REFERENCES | 第115-119页 |
| 第4章 全文结论 CONCLUSION AND RECOMMENDATIONS | 第119-121页 |
| 致谢ACKNOWLEDGEMENTS | 第121-122页 |
| APPENDICES | 第122-144页 |
| 作者简历 AUTHOR CV | 第144页 |
