| 摘要 | 第8-10页 |
| Abstract | 第10-12页 |
| 1 Introduction | 第13-29页 |
| 1.1 Chicken as experimental model | 第13页 |
| 1.2 Selenium | 第13-21页 |
| 1.2.1 Form and distribution | 第13-14页 |
| 1.2.2 Se deficiency and disease | 第14-15页 |
| 1.2.3 Selenoproteins | 第15-16页 |
| 1.2.4 Expression pattern of Selenoproteins in Se deficiency | 第16-17页 |
| 1.2.5 Selenoproteins knockdown and/or over-expression | 第17-19页 |
| 1.2.6 Autophagy and Apoptosis | 第19-21页 |
| 1.3 Se and Male Reproduction | 第21-28页 |
| 1.3.1 Selenium role in Testes | 第21-22页 |
| 1.3.2 Oxidative stress in Testes | 第22-23页 |
| 1.3.3 Functional importance of Sertoli cells | 第23-27页 |
| 1.3.4 Selenoprotein U and its role in reproduction | 第27-28页 |
| 1.4 Objectives of Research | 第28-29页 |
| 2 Material and methods | 第29-35页 |
| 2.1 In-vitro Experiment | 第29-31页 |
| 2.1.1 Sertoli Cell culture | 第29页 |
| 2.1.2 si RNA transfection of Sertoli cells | 第29-30页 |
| 2.1.3 Total RNA Extraction | 第30页 |
| 2.1.4 Primers Design | 第30页 |
| 2.1.5 Quantitative Real-time PCR | 第30-31页 |
| 2.1.6 Western Blotting | 第31页 |
| 2.1.7 Electron Microscopy | 第31页 |
| 2.2 In-vivo Experiment | 第31-34页 |
| 2.2.1 Birds and experimental design | 第31-32页 |
| 2.2.2 Total RNA Extraction | 第32页 |
| 2.2.3 Primers Design | 第32页 |
| 2.2.4 Quantitative Real-time PCR | 第32-33页 |
| 2.2.5 Measurement of Oxidative stress | 第33页 |
| 2.2.6 Hematoxylin and Eosin Staining | 第33-34页 |
| 2.3 Statistical analysis | 第34-35页 |
| 3 Results | 第35-60页 |
| 3.1 In-vitro Experiment | 第35-44页 |
| 3.1.1 Establishment of Sel U knockdown model | 第35-36页 |
| 3.1.2 mRNA expression of autophagy and cyto-skeleton genes in SCs | 第36页 |
| 3.1.3 mRNA expression of apoptosis genes in SCs | 第36-37页 |
| 3.1.4 mRNA expression of PI3K-Akt signaling pathway genes in SCs | 第37-38页 |
| 3.1.5 mRNA expression of Regulatory factors in SCs | 第38-39页 |
| 3.1.6 mRNA expressions of Junction associated genes in SCs | 第39-40页 |
| 3.1.7 Protein expression of autophagy genes in SCs | 第40-41页 |
| 3.1.8 Protein expression of apoptosis genes in SCs | 第41-42页 |
| 3.1.9 Protein expression of PI3K-Akt signaling pathway genes in SCs | 第42-43页 |
| 3.1.10 Ultrastructure analysis | 第43-44页 |
| 3.2 In-vivo Experiment | 第44-60页 |
| 3.2.1 mRNA expressions of selenoproteins in Testis tissues | 第44-49页 |
| 3.2.2 mRNA expression of PI3K-Akt and MEK-ERK pathway genes in Testis tissues | 第49-51页 |
| 3.2.3 mRNA expression of Regulatory factors in Testis tissues | 第51-52页 |
| 3.2.4 mRNA expression of Junction associated genes in Testis tissues | 第52-53页 |
| 3.2.5 Measurement of oxidative stress in Testis tissues | 第53-57页 |
| 3.2.6 Histo-pathology of Testis tissues | 第57-60页 |
| 4 Discussion | 第60-74页 |
| 4.1 Role of Selenoprotein U in autophagy and apoptosis in chicken Sertoli cells (in-vitro) | 第60-63页 |
| 4.2 Effect of Se deficiency on the expression of selenoproteins in chicken testis (in-vivo) | 第63-66页 |
| 4.3 Effect of Se deficiency on the oxidative status and histopathology in chicken testis (in-vivo) | 第66-69页 |
| 4.4 Effect of Se deficiency and Sel U silencing on the expression of Regulatory factors andJunction associated genes in chicken Sertoli cells (in-vitro and in-vivo) | 第69-74页 |
| 5 Conclusion | 第74-75页 |
| Acknowledgement | 第75-76页 |
| References | 第76-99页 |
| Papers Published in the period of PhD Study | 第99-100页 |
