| 摘要 | 第4-10页 |
| Abstract | 第10-17页 |
| Chapter 1 General review | 第21-55页 |
| 1.1 Introduction | 第21-23页 |
| 1.2 Bioleaching microorganisms | 第23-33页 |
| 1.2.1 Mesophiles | 第24-26页 |
| 1.2.2 Moderate Thermophiles | 第26-29页 |
| 1.2.3 Thermophiles | 第29-31页 |
| 1.2.4 Acidophilic heterotrophs | 第31-33页 |
| 1.3 Leaching bacteria isolation techniques | 第33-35页 |
| 1.4 Microbial population analysis methods | 第35-45页 |
| 1.4.1 Culture-dependent techniques | 第35-36页 |
| 1.4.2 PCR-based Molecular Methods | 第36-41页 |
| 1.4.3 Nucleic acid hybridization and fluorescent in situ hybridization(FISH) | 第41-42页 |
| 1.4.4 Microarrays techniques | 第42-43页 |
| 1.4.5 Emerging molecular methods(Omic approaches) | 第43-45页 |
| 1.5 Microbe-microbe interactions | 第45-48页 |
| 1.5.1 Competition | 第46页 |
| 1.5.2 Synergism | 第46-47页 |
| 1.5.3 Mutualism | 第47-48页 |
| 1.5.4 Predation | 第48页 |
| 1.6 Microbe-mineral interactions | 第48-53页 |
| 1.6.1 The two pathways | 第49-51页 |
| 1.6.2 Microbe-mineral interface:Role of the EPS | 第51-53页 |
| 1.7 Research aims,significances and contents | 第53页 |
| 1.8 Originality of the thesis topic | 第53-55页 |
| Chapter 2 Isolation of leaching bacteria using modified double layer technique | 第55-66页 |
| 2.1 Introduction | 第55-56页 |
| 2.2 Materials and methods | 第56-58页 |
| 2.2.1 Samples and media | 第56页 |
| 2.2.2 Yeast isolation | 第56-57页 |
| 2.2.3 Leaching bacteria isolation | 第57页 |
| 2.2.4 Phylogenetic analysis | 第57-58页 |
| 2.2.5 Morphological,physiological and biochemical characterization | 第58页 |
| 2.3 Results and discussion | 第58-65页 |
| 2.3.1 Phylogenetic and physiological characterization of the yeast strain | 第58-60页 |
| 2.3.2 Effect of Candida digboiensis NB on leaching bacteria growth on double layer agar plate | 第60-61页 |
| 2.3.3 Isolation and identification of the leaching bacteria | 第61-63页 |
| 2.3.4 Physiological characterization of the leaching bacteria | 第63-65页 |
| 2.4 Conclusions | 第65-66页 |
| Chapter 3 Impact of C.digboiensis on leaching bacteria under organic acid stress | 第66-75页 |
| 3.1 Introduction | 第66-67页 |
| 3.2 Materials and methods | 第67-68页 |
| 3.2.1 Microorganisms and cultures conditions | 第67页 |
| 3.2.2 Growth and activity of the different strains under organic acid stress | 第67页 |
| 3.2.3 Effect of C.digboiensis on iron and sulfur oxidation rates under organic acids stress | 第67-68页 |
| 3.2.4 Effect of the introduction of C.digboiensis in bioleaching of pyrite under organic acid stress | 第68页 |
| 3.2.5 Analytical methods | 第68页 |
| 3.3 Results and discussions | 第68-74页 |
| 3.3.1 Response of the strains to various concentrations of organic acid | 第68-69页 |
| 3.3.2 Organic acids consumption and microbial population dynamics | 第69-71页 |
| 3.3.3 Effect of C.digboiensis on Microbial activity under organic acid stress | 第71页 |
| 3.3.4 Simulation of C.digboiensis role in sludge bioleaching | 第71-74页 |
| 3.4 Conclusions | 第74-75页 |
| Chapter 4 Effect of acidophilic yeast strain on leaching efficiency | 第75-84页 |
| 4.1 Introduction | 第75-76页 |
| 4.2 Materials and methods | 第76-78页 |
| 4.2.1 Microorganisms and cultures conditions | 第76页 |
| 4.2.2 Minerals sample | 第76页 |
| 4.2.3 Effect of C.digboiensis on iron and sulfur oxidation rate | 第76-77页 |
| 4.2.4 Bioleaching experiments | 第77页 |
| 4.2.5 Analytical methods | 第77-78页 |
| 4.3 Results and disussions | 第78-83页 |
| 4.3.1 Changes in pH and Eh | 第78-79页 |
| 4.3.2 Microbial population dynamics analysis | 第79页 |
| 4.3.3 DOM accumulation | 第79-80页 |
| 4.3.4 Iron and copper extraction rates | 第80-81页 |
| 4.3.5 Effect of C.digboiensis on iron and sulfur oxidation rate | 第81-82页 |
| 4.3.6 Comparison between C.digboiensis sp.and Acidiphilium sp.in bioleaching | 第82-83页 |
| 4.4 Conclusion | 第83-84页 |
| Chapter 5 Comparison of native and exogenous bacteria for the bioleaching of low-gradecopper ores | 第84-103页 |
| 5.1 Introduction | 第84-85页 |
| 5.2 Materials and methods | 第85-88页 |
| 5.2.1 Bacterial strains and cultures conditions | 第85-86页 |
| 5.2.2 Mineral samples | 第86-87页 |
| 5.2.3 Bioleaching experiments | 第87页 |
| 5.2.4 Analytical methods | 第87-88页 |
| 5.3 Results and discussions | 第88-102页 |
| 5.3.1 Physiological characteristics | 第88-94页 |
| 5.3.2 Bioleaching efficiency | 第94-102页 |
| 5.4 Conclusions | 第102-103页 |
| Chapter 6 General conclusions | 第103-105页 |
| References | 第105-125页 |
| Achievements | 第125-126页 |
| Acknowledgements | 第126-127页 |
