| 摘要 | 第6-7页 |
| ABSTRACT | 第7-8页 |
| ABBREVIATIONS | 第12-13页 |
| CHAPTER ONE INTRODUCTION | 第13-21页 |
| 1.1 Cotton | 第13-14页 |
| 1.2 AAI gene family: | 第14-15页 |
| 1.3 Brassinosteroids(BRs) | 第15-17页 |
| 1.4 Early Flowering | 第17-18页 |
| 1.5 Biomass | 第18-19页 |
| 1.6 Hypothesis | 第19-20页 |
| 1.7 Research Objectives | 第20-21页 |
| CHAPTER TWO Genome-wide identification of GhAAI genes and GhAAI66 triggers a phase transition to induce early flowering | 第21-40页 |
| 2.1 MATERIALS AND METHODS | 第21-23页 |
| 2.1.1 Gene identification and analysis of conserved residues | 第21页 |
| 2.1.2 Phylogenetic analysis and determination of protein motif distribution and gene structure | 第21-22页 |
| 2.1.3 Chromosomal location,gene duplication,and synteny analysis | 第22页 |
| 2.1.4 Vector construction and generation of transgenic lines | 第22页 |
| 2.1.5 RNA-seq,KEGG,and GO analysis | 第22-23页 |
| 2.1.6 Plant material,hormone treatments,and qRT-PCR analysis | 第23页 |
| 2.2 RESULTS | 第23-34页 |
| 2.2.1 Identification and analysis of conserved amino acid residues in AAIs | 第23-24页 |
| 2.2.2 Phylogenetic analysis of AAI genes | 第24-26页 |
| 2.2.3 Protein motifs and gene structure analysis | 第26页 |
| 2.2.4 Chromosomal location,gene duplication,and synteny analysis of GhAAI genes | 第26-28页 |
| 2.2.5 Tissue specific expression pattern and responses to phytohormone treatment | 第28-30页 |
| 2.2.6 Ectopic expression of GhAAI66 regulates phase transition to induce early flowering | 第30-31页 |
| 2.2.7 GhAAI66 integrates multiple flower signaling pathways to induce early flowering | 第31-34页 |
| 2.3 DISCUSSION | 第34-40页 |
| 2.3.1 AAI genes were highly conserved during evolution | 第34-35页 |
| 2.3.2 Expansion and duplication of GhAAI gene family during evolution | 第35-36页 |
| 2.3.3 GhAAI genes have ubiquitous expression in tissues and regulated hormone treatments | 第36-37页 |
| 2.3.4 GhAAI66 regulates early flowering in Arabidopsis | 第37-40页 |
| CHAPTER THREE NGhAAI66 mediates BR signaling and increase biomass | 第40-56页 |
| 3.1 MATERIALS AND METHODS | 第40-41页 |
| 3.1.1 Plant material and Fox hunting for bri1-5 suppressor | 第40页 |
| 3.1.2 Construction of expression vectors | 第40页 |
| 3.1.3 Plant treatment and hypocotyl measurement | 第40页 |
| 3.1.4 Generation of promoter-GUS transgenic plants | 第40-41页 |
| 3.1.5 Biomass analysis | 第41页 |
| 3.1.6 Gene expression by qRT-PCR and statistical analysis | 第41页 |
| 3.2 RESULTS | 第41-51页 |
| 3.2.1 Identification of GhAAI66 gene | 第41-42页 |
| 3.2.2 GhAAI66 expressed ubiquitously | 第42-44页 |
| 3.2.3 GhAAI66 is a dominant genetic suppressor of bri1- | 第44-45页 |
| 3.2.4 GhAAI66 mediate multiple aspects of plant growth and development in Arabidopsis | 第45-47页 |
| 3.2.5 GhAAI66 plays positive role in BR signaling pathway | 第47-49页 |
| 3.2.6 GhAAI66 is the direct target of GhBES | 第49页 |
| 3.2.7 GhAAI66 enhance vegetative biomass and seed size in Arabidopsis | 第49-51页 |
| 3.3 DISCUSSION | 第51-56页 |
| 3.3.1 GhAAI66 is a dominant genetic suppressor of bri1-5 and act downstream of BRI1 in BR signaling | 第51-52页 |
| 3.3.2 A functional BRI1 is required for GhAAI66 to mediates plant growth and development | 第52-53页 |
| 3.3.3 GhAAI66 functions through GhBES1 to regulate BR signaling pathway | 第53-54页 |
| 3.3.4 GhAAI66 enhance vegetative capacity and seed size in Arabidopsis | 第54-56页 |
| CHAPTER FOUR CONCLUSION | 第56-58页 |
| REFERENCES | 第58-70页 |
| SUPPLEMENTARY DATA | 第70-81页 |
| ACKNOWLEDGEMENT | 第81-82页 |
| CURRICULUM VITAE | 第82-83页 |
