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陆地棉耐盐性的QTL定位和候选基因鉴定

摘要第6-7页
abstract第7-8页
DEDICATIONS第9-17页
Abbreviations第17-19页
CHAPTER1:Introduction第19-49页
    1.1 General Description of Cotton第19页
    1.2 Current status of cotton production and Usage第19-21页
        1.2.1 FAS USDA projection第21页
    1.3 Phylogeny of Cotton第21-22页
    1.4 Characteristics,Contribution,and Origin第22-23页
    1.5 Genetic diversity in the cultivated cotton第23-25页
    1.6 Optimum conditions for cotton growth第25-26页
    1.7 Plant Stress第26-27页
        1.7.1 Drought Stress第26-27页
        1.7.2 Salinity Stress第27页
    1.8 Salt-affected area第27-28页
    1.9 Development of Salinity第28-29页
    1.10 Major types of Salts第29页
    1.11 Estimation of salinity第29-31页
        1.11.1 Mapping and Monitoring of Salinity areas第29页
        1.11.2 Need for Monitoring and Mapping?第29页
        1.11.3 Technology used for soil salinity mapping第29-30页
        1.11.4 Remote Sensing第30-31页
            1.11.4.1 Advantages第30页
            1.11.4.2 Requirements第30页
            1.11.4.3 Vegetation reflectance第30-31页
    1.12 Laboratory Method第31页
    1.13 Classification of soil based on EC第31-32页
    1.14 Classification of Soil By USD Soil Laboratory第32-33页
        1.14.1 Saline Soils:第32页
        1.14.2 Sodic Soils第32页
        1.14.3 Saline-Sodic Soils:第32-33页
    1.15 Comparison of Estimation of Salinity methods第33页
    1.16 Effect of salinity on Plants第33页
    1.17 Effect of Salinity on Cotton Growth第33-35页
        1.17.1 Germination and Emergence第34页
        1.17.2 Root and Shoot第34页
        1.17.3 Effect on Other Parts第34-35页
    1.18 Salinity Tolerance第35页
    1.19 Plant Response to Salinity第35-37页
    1.20 Identification Method of Salt Tolerance of Cotton第37页
    1.21 Evaluation of Salt tolerance Indicators of Cotton:第37-39页
    1.22 Salt tolerance of Plants(Molecular level)第39页
    1.23 Reclamation Strategies第39-40页
    1.24 Live with Salinity(Saline Agriculture)第40-43页
        1.24.1 Introduction Natural salt-tolerant plants第40-41页
        1.24.2 Developing GMO Plants:第41-43页
            1.24.2.1 Conventional& Molecular Breeding第41-42页
            1.24.2.2 Advances in Molecular breeding第42-43页
    1.25 QTL mapping第43页
    1.26 Requirements for QTL mapping第43-44页
    1.27 Genetic Markers第44-47页
        1.27.1 Morphological marker第44页
        1.27.2 Biochemical markers第44页
        1.27.3 DNA based markers第44-47页
            1.27.3.1 Hybridization-based markers第44页
            1.27.3.2 Polymerase chain reaction(PCR)based markers第44-47页
                1.27.3.2.1 Simple Sequence Repeats(SSR)第45页
                1.27.3.2.2 Single Nucleotide Polymorphisms(SNP)第45-47页
    1.28 Mapping Population第47页
    1.29 Candidate Genes第47-48页
    1.30 Statement of Purpose第48页
    1.31 Study Objectives:第48-49页
CHAPTER2:MATERIALS AND METHODS第49-62页
    2.1 Plant Materials第49页
    2.2 Mapping Population第49-50页
    2.3 Experimental Conditions第50页
    2.4 Experimental design and procedures第50-51页
        2.4.1 Design and Layout第50页
        2.4.2 Seed treatment第50-51页
    2.5 Soil Culture Experiment第51-53页
        2.5.1 Germination Test第51-52页
            2.5.1.1 Treatments第51页
            2.5.1.2 Salt Calculations第51-52页
            2.5.1.3 Germination/Emergence Data(GR)第52页
        2.5.2 Seedling Test第52页
            2.5.2.1 Basal treatment第52页
            2.5.2.2 Third Leaf Stage Treatment150mM(E3)第52页
        2.5.3 Solution Culture Experiment第52-53页
            2.5.3.1 Treatments第53页
            2.5.3.2 Germination第53页
            2.5.3.3 Transplantation of Seedlings第53页
            2.5.3.4 Salt Treatment第53页
    2.6 Data collection of phenotypic traits第53-55页
        2.6.1 Shoot Length(cm)第54页
        2.6.2 Root Length(cm)第54页
        2.6.3 Shoot Fresh Weight(g)第54页
        2.6.4 Shoot Dry weight(g)第54页
        2.6.5 Root Fresh Weight(g)第54页
        2.6.6 Root Dry Weight(g)第54页
        2.6.7 Leaf Fresh weight(g)第54页
        2.6.8 Stem Weight(g)第54-55页
        2.6.9 Leaf Imbibed weight(g)第55页
        2.6.10 Leaf Dry weight(g)第55页
        2.6.11 Relative water contents(%)第55页
        2.6.12 Chlorophyll Contents(SPAD)第55页
        2.6.13 Electrical Conductivity meter(dS/cm)第55页
        2.6.14 Total Biomass/Total Plant Weight(g)第55页
        2.6.15 Root Shoot Ratio第55页
    2.7 Phenotypic data analysis第55-56页
        2.7.1 Correlation第56页
            2.7.1.1 Correlogram第56页
    2.8 Genotyping and Map Construction第56-60页
        2.8.1 DNA extraction,quantification,and qualification第56页
        2.8.2 Microsatellites genotyping第56-57页
        2.8.3 Polymerase chain reaction and electrophoresis第57页
        2.8.4 SNP Chip-based Sequencing:第57-58页
        2.8.5 Genetic Map第58-60页
    2.9 QTL Mapping第60-62页
        2.9.1 Nomenclature and QTL information第60页
        2.9.2 QTL Comparison第60页
        2.9.3 Candidate Genes Identification第60-62页
CHAPTER3:RESULTS第62-109页
    3.1 Phenotypic data analysis第62-85页
        3.1.1 Evaluation of Parental lines第62-64页
        3.1.2 Germination第64-69页
            3.1.2.1 Germination of Parental lines and Control across different treatments第64-65页
            3.1.2.2 Frequency Distribution/Descriptive statistics of whole population第65-66页
            3.1.2.3 Correlation第66-67页
            3.1.2.4 Biplot Analysis第67页
            3.1.2.5 ANOVA(Analysis of Variance)One Way第67-69页
        3.1.3 Seedling Experiment第69页
            3.1.3.1 Overall Description and Comparison(T1,T2 and T4)第69页
            3.1.3.2 Overall Description and ccomparison of E1 and E2 in aquaculture第69页
        3.1.4 Correlation(r)matrix(Pearson(n))第69-85页
            3.1.4.1 Treatment1 (Control)第69-70页
            3.1.4.2 Treatment T2(75mM)第70页
            3.1.4.3 Treatment T4(125mM)第70页
            3.1.4.4 Treatment5 in E3(150 mM)第70页
            3.1.4.5 Treatment5 in E1(Hydroponic culture)第70页
            3.1.4.6 Treatment5 in E2(Hydroponic culture)第70-82页
            3.1.4.7 Principal component analysis(PCA)第82-84页
            3.1.4.8 Evaluation of Salt tolerant Lines第84-85页
    3.2 QTL Mapping for Salinity tolerance第85-100页
        3.2.1 QTL Mapping第85页
        3.2.2 Stable QTLs第85页
        3.2.3 Stable QTLs making clusters第85-86页
        3.2.4 Already Reported QTLs第86页
        3.2.5 Cluster QTLs第86-100页
    3.3 Candidate genes第100-109页
        3.3.1 Gene Ontology第100-101页
        3.3.2 KEGG pathway第101-102页
        3.3.3 Inter-Pro第102-106页
        3.3.4 The biological function annotations of twenty-one selected candidate genes related to salinity tolerance第106-109页
CHAPTER4:DISCUSSIONS第109-116页
    4.1 QTL mapping on salinity tolerance第109页
    4.2 Germination第109-113页
        4.2.1 Seedling Experiment第110-112页
        4.2.2 Correlation:第112-113页
    4.3 Genetic map and QTL mapping第113-114页
    4.4 Candidate Genes第114-116页
CONCLUSION第116-117页
References(参考文献)第117-134页
Acknowledgements第134-135页
Curriculum Vitae(个人简介)第135-137页
Appendix第137-184页

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