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镉低积累小白菜食品安全的农艺调控机制

Acknowledgements第8-10页
Abbreviations第10-16页
ABSTRACT第16-18页
摘要第19-21页
CHAPTER 1 General Introduction and Review of Literature第21-28页
    1.1 Cadmium in Soil第21-22页
    1.2 Effect of Cd on essential trace metals bioavailability in the soil第22-23页
    1.3 Cadmium in Food and Dietary toxicity第23-24页
    1.4 Bioaccessibility of Cd to human第24页
    1.5 Remediation of Cd contaminated soils through Amendments第24-26页
    1.6 Using biochar as soil amendment第26-28页
CHAPTER 2 Bioavailability and bioaccessibility of Cd in low and high Cd uptake affinity Cultivars of Brassica rapa ssp.chinensis L.(pakchoi)using an in vitro physiologically based extraction test第28-41页
    2.1 Introduction第28-29页
    2.2 Materials and methods第29-32页
        2.2.1 Soil characterization and contamination第29-30页
        2.2.2 Mehlich-3 extraction of Cd第30页
        2.2.3 Plants growth and Cd determination in shoots第30-31页
        2.2.4 Physiologically-based extraction test(PBET)第31页
        2.2.5 Calculation of bioaccessibility第31-32页
        2.2.6 Statistical Analysis第32页
    2.3 Results第32-38页
        2.3.1 Availability of soil Cd第32页
        2.3.2 Dry biomass and Cadmium concentration in plant shoots第32-34页
        2.3.3 Bioaccessibility of Cd from pak choi cultivars through PBET第34-38页
    2.4 Discussion第38-39页
    2.5 Conclusion第39-41页
CHAPTER 3 Study of Ultrastructural localization of cadmium,plant growth parameters and amino acid contents in two Brassica rapa ssp. chinensis L.(pak choi)cultivars differing in cadmium accumulation第41-61页
    3.1 Introduction第41-42页
    3.2 Material and Method第42-44页
        3.2.1 Plant material and growth conditions第42页
        3.2.2 Morphological parameters第42-43页
        3.2.3 Determination of Cd contents第43页
        3.2.4 Leaf gas exchange analysis第43页
        3.2.5 Free amino acids analysis第43页
        3.2.6 Transmission electron microscopy第43-44页
        3.2.7 Statistical analysis第44页
    3.3 Results第44-57页
        3.3.1 Effects of Cd on the plant growth第44-45页
        3.3.2 Cd contents in the plants第45页
        3.3.3 Effects of Cd on photosynthetic parameters第45-48页
        3.3.4 Free amino acids in plants第48页
        3.3.5 Cd-toxicity induces ultrastructural changes第48-57页
    3.4 Discussion第57-60页
    3.5 Conclusion第60-61页
CHAPTER 4 Effect of humic acid amendment on cadmium bioavailability and accumulation by pak Choi (Brassica rapa ssp. chinensis L.)to alleviate dietary toxicity risk第61-75页
    4.1 Introduction第61-62页
    4.2 Materials and methods第62-64页
        4.2.1 Study sites and soil material collection第62页
        4.2.2 Pot experiment第62-63页
        4.2.3 Analysis of Cd in the soils and plants samples第63页
        4.2.4 Statistical analysis第63-64页
    4.3 Results and discussion第64-73页
        4.3.1 Effect of HA-amendment on soil characteristics第64页
        4.3.2 Effect of HA-amendment on soil Cd bioavailability第64-68页
        4.3.3 Effect of HA on plant dry biomass第68页
        4.3.4 Soil Cd thresholds with HA amendment for potential dietary toxicity risk in pak choi第68-69页
        4.3.5 Effect of HA-amendment on Cd accumulation in pak choi第69-73页
    4.4 Conclusion第73-75页
Chapter 5 Impact of Different Feedstocks Derived Biochar Amendment with Cadmium Low uptake Affinity Cultivar of pak choi(Brassica rapa ssb.chinensis L.)on Phytoavoidation of Cd to reduce potential dietary toxicity第75-97页
    5.1 Introduction第75-77页
    5.2 Materials and Methods第77-80页
        5.2.1 Biochar production and physicochemical characterization第77-78页
        5.2.2 Soil and amendments第78页
        5.2.3 Mehlich-3 extraction第78页
        5.2.4 Plant and greenhouse experiment第78-79页
        5.2.5 Metal analysis of soil and plant samples第79页
        5.2.6 Statistical analysis第79-80页
    5.3 Results第80-88页
        5.3.1 Characteristics of biochar第80-82页
        5.3.2 Effect of biochar on soil pH第82页
        5.3.3 Effect of biochar on Cd and trace metals bioavailability第82-83页
        5.3.4 Effect of biochar and cultivars on Cd accumulation第83-84页
        5.3.5 Effect of biochar amendment on essential trace metals accumulation第84页
        5.3.6 Effect of biochar on dry biomass of cultivars第84-88页
    5.4 Discussion第88-95页
    5.5 Conclusion第95-97页
CHAPTER 6 Cow manure and cow manure-derived biochar application as a soil amendment for reducing cadmium availability and accumulation by pak choi (Brassica rapa ssp. chinensis L.)in acidic red soil第97-112页
    6.1 Introduction第97-98页
    6.2 Materials and methods第98-101页
        6.2.1 Biochar production and characterization第98-99页
        6.2.2 Mehlich-3 extraction for analysis of soil nutrients第99-100页
        6.2.3 Soil Preparation第100页
        6.2.4 Plant and greenhouse experiments第100页
        6.2.5 Analysis of Cd and trace metals in plants and soil第100-101页
        6.2.6 Statistical analysis第101页
    6.3 Results第101-107页
        6.3.1 Characteristics of cow manure-derived biochar第101-104页
        6.3.2 Plant dry biomass in CMB and CM amended soils第104-106页
        6.3.3 Bioaccumulation of Cd and trace metals in pak choi第106-107页
    6.4 Discussion第107-111页
    6.5 Conclusion第111-112页
CHAPTER 7第112-114页
    7.1 Major Findings and Future Perspectives第112-114页
        7.1.1 Major Findings第112-113页
        7.1.2 Future Perspectives第113-114页
References第114-137页
List of publications第137页

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