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L-茶氨酸日粮添加水平对肉鸡生长性能、肉品质及免疫反应的影响

ABSTRACT第8-10页
摘要第11-16页
CHAPTER 1 REVIEW OF LITERATURE第16-33页
    1.1 ADVANCE RESEARCH OF L-THEANINE第16-19页
    1.2 BIOSYNTHESIS OF THEANINE第19-20页
    1.3 SOURCES OF L-THEANINE第20-21页
    1.4 METABOLISM OF L-THEANINE (AA)第21页
    1.5 CHEMICAL STRUCTURE OF L-THEANINE第21-22页
    1.6 PHARMACOKINETICS OF L-THEANINE第22-23页
    1.7 BENEFICIAL EFFECTS OF L-THEANINE第23页
    1.8 LIPID-LOWERING PROPERTIES第23-24页
    1.9 ANTI-HYPERTENSION EFFECTS第24页
    1.10 IMMUNOMODULATORY EFFECTS第24-25页
    1.11 INCREASED RESISTANCE AGAINST PATHOGENIC BACTERIA第25页
    1.12 NEUROPROTECTIVE AND MEMORY ENHANCEMENT PROPERTIES第25-26页
    1.13 RELAXATION PROPERTIES第26页
    1.14 HEPATOPROTECTIVE AND ANTIOXIDANT EFFECTS第26-28页
    1.15 USAGE OF TEA PLANT AMINO ACID L-THEANINE IN POULTRY第28-29页
    1.16 BROILERS CHICKENS第29-30页
    1.17 LAYING HENS第30-31页
    1.18 TOXICOLOGY EFFECT OF L-THEANINE第31-32页
    FLOW CHART OF OUR EXPERIMENTS第32-33页
CHAPTER 2 INFLUENCE OF GRADED LEVELS OF L-THEANINEDIETARY SUPPLEMENTATION ON GROWTH PERFORMANCE,MEAT QUALITY, AND IMMUNE RESPONSE OF BROILERCHICKENS第33-55页
    2.1 INTRODUCTION第34-35页
    2.2 OBJECTIVE第35-36页
    2.3 METHODOLOGY第36-42页
        2.3.1 Ethical Statement第36页
        2.3.2 Design and feeding management第36页
        2.3.3 Diet formulation第36-37页
        2.3.4 Housing and managements第37-38页
        2.3.5 Sample Collection and Processing第38-39页
        2.3.6 Immune response and antioxidant status第39-40页
        2.3.7 Blood chemistry第40页
        2.3.8 The histomorphometry of small intestinal mucosa第40-41页
        2.3.9 The histomorphometry of pectoral and gastrocnemius muscles第41页
        2.3.10 Statistical analysis第41-42页
    2.4 RESULTS AND DISCUSSIONS第42-54页
        2.4.1 Growth performance第42-43页
        2.4.2 Carcass traits第43-45页
        2.4.3 Meat quality第45-46页
        2.4.4 Mucosal histomorphometry of small intestine第46-49页
        2.4.5 Histomorphometry of pectoral and gastrocnemius muscles第49-50页
        2.4.6 Effect on blood chemistry第50-52页
        2.4.7 Immune response第52-54页
    2.5 CONCLUSIONS第54-55页
CHAPTER 3 16S RRNA SEQUENCING REVEALS MODULATION OFINTESTINAL MICROBIOME AND IMMUNE RESPONSE BYDIETARY L-THEANINE SUPPLEMENTATION IN BROILERCHICKENS第55-84页
    3.1 INTRODUCTION第56-59页
    3.2 OBJECTIVE第59页
    3.3 METHODOLOGY第59-64页
        3.3.1 DNA Extraction, 16S r RNA amplification and Miseq sequencing第59-60页
        3.3.2 Relative changes in lactobacillus as compared to bactericides第60页
        3.3.3 Data analysis第60页
        3.3.4 Expression of ZO-1, Occuldin and Claudin-第60-61页
        3.3.5 Experimental design第61-64页
        3.3.6 Sample collection and processing第64页
    3.4 RESULTS第64-80页
        3.4.1 Analysis of Operational Taxonomic Units (OTUs) and Abundance of IntestinalMicrobiome第64-66页
        3.4.2 Distribution of OTUs of intestinal mucosal flora using Venn diagrams第66-67页
        3.4.3 Analysis of species diversity index of intestinal mucosal flora第67-68页
        3.4.4 Changes in microbial community of intestinal mucosa in response to treatment第68-76页
        3.4.5 Detection of changes in Lactobacillus and Bacteroides by using q PCR第76-77页
        3.4.6 Changes in m RNA expression of mucosal structural proteins第77-78页
        3.4.7 Effects of L-theanine on intestinal immunity第78-80页
    3.5 DISCUSSION第80-83页
    3.6 CONCLUSION第83-84页
REFERENCES第84-104页
ACKNOWLEDGEMENTS第104-105页
BIOGRAPHY OF AUTHOR第105-107页

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