| Abstract | 第6-7页 |
| 中文摘要 | 第8-10页 |
| Abbreviations | 第10-12页 |
| Chapter Ⅰ Review: Polarity proteins, Vesicular tracking, Cell migration, Border cellmigration | 第12-54页 |
| 1. Polarity proteins | 第13-22页 |
| 1.1 Polarity proteins in epithelial cell | 第13-16页 |
| 1.2 Crumb complex | 第16-17页 |
| 1.3 Par complex | 第17-19页 |
| 1.4 Par complex and cytoskeleton | 第19-22页 |
| 2. Vesicular trafficking | 第22-28页 |
| 2.1 Endocytosis | 第22-25页 |
| 2.2 endocytic recycling | 第25-27页 |
| 2.3 Exocyst | 第27-28页 |
| 3. Cell migration | 第28-33页 |
| 3.1 Actin-based protrusions | 第28-30页 |
| 3.2 Rho GTPase | 第30-32页 |
| 3.3 Variant polarities in single cell or collective cells | 第32-33页 |
| 4. Border cell migration | 第33-44页 |
| 4.1 Border cells in Drosophila | 第34-35页 |
| 4.2 The initiation of border cell migration | 第35-39页 |
| 4.2.1 Recruit border cells | 第35-37页 |
| 4.2.2 Timing of migration | 第37-38页 |
| 4.2.3 Delamination of border cells | 第38-39页 |
| 4.3 Border cell migration | 第39-42页 |
| 4.3.1 Guidance of border cells | 第39-40页 |
| 4.3.2 Power of border cell migration | 第40-42页 |
| 4.4 Polarities of border cell cluster | 第42-44页 |
| References | 第44-54页 |
| Chapter Ⅱ Apical Complex Molecules Play Essential and Novel Roles in Collective CellMigration | 第54-112页 |
| Introduction | 第55-57页 |
| Materials and Methods | 第57-61页 |
| Drosophila genetics | 第57-58页 |
| Immunostaining and microscopy | 第58-59页 |
| Live-cell imaging and photomanipulation | 第59-60页 |
| Migration analysis of border cells | 第60页 |
| Quantification of protrusions | 第60-61页 |
| Quantification of fluorescence intensity | 第61页 |
| Results | 第61-103页 |
| Section 2.1 Spatial pattern and functions of apical complex in BCs | 第61-66页 |
| 2.1.1 Apical polarity proteins and AJ proteins distribute at the apical junctions and outercortex of border cell cluster | 第62-64页 |
| 2.1.2 Apical polarity proteins are required for border cell migration | 第64-66页 |
| Section 2.2 Disruption of Crumb complex leads to ectopic actin rich protrusions | 第66-74页 |
| 2.2.1 Disrupting any member of Crb complex leads to ectopic actin patches | 第66-68页 |
| 2.2.2 Delayed border cells possess more actin patches | 第68-69页 |
| 2.2.3 Ectopic actin patches are proved to be actin-based protrusions and can dynamicallyextend and retract | 第69-73页 |
| 2.2.4 Ectopic protrusions are not caused by loss of cell-cell communication | 第73-74页 |
| Section 2.3 Apical polarity molecules regulate the direction of border cell cluster | 第74-76页 |
| Section 2.4 aPKC accumulates at the ectopic actin patches | 第76-81页 |
| 2.4.1 Disruption of Crb complex specially causes aPKC and Par6 accumulate at those actinpatches | 第77-79页 |
| 2.4.2 Down-regulating Par6 or Cdc42 but not Baz also induce ectopic protrusions withdissociated aPKC inside | 第79-81页 |
| Section 2.5 aPKC promotes actin polymerization and protrusions formation in BCs | 第81-87页 |
| 2.5.1 Decreasing aPKC inhibits actin polymerization and impedes extension of both leadingand non-leading protrusions | 第81-82页 |
| 2.5.2 Active aPKC promotes actin polymerization and protrusions formation and biases cellto be leader | 第82-85页 |
| 2.5.3 Live imaging shows the effect of aPKC on the morphology of BCs | 第85-87页 |
| Section 2.6 aPKC activatesRac through downstream Sif | 第87-91页 |
| 2.6.1 Sif acts downstream of aPKC in BCs | 第87-90页 |
| 2.6.2 Rac acts downstream of aPKC and Sif | 第90-91页 |
| Section 2.7 Sif localizes at the basolateral side of BCs and co-localizes with aPKC at theleading edge | 第91-94页 |
| Section 2.8 aPKC-Sif-Rac is required for ectopic protrusions caused by patj.RNAi | 第94-98页 |
| 2.8.1 The signaling of aPKC-Sif-Rac is responsible for the ectopic protrsions | 第94-96页 |
| 2.8.2 Active Rac is accumulated in ectopic protrusions | 第96-98页 |
| Section 2.9 Vesicle trafficking is responsible for membrane localization of apical polarityproteins | 第98-103页 |
| 2.9.1 Disruption of vesicle trafficking reveals periphery membrane localization of aPKC,Par6 and Crb | 第98-101页 |
| 2.9.2 Live imaging shows the movement of apical polarity proteins in BCs | 第101-103页 |
| Discussion | 第103-108页 |
| References | 第108-112页 |
| Acknowledgements | 第112-113页 |
| Publications | 第113-115页 |
