| Abstract | 第4-5页 |
| 摘要 | 第6-11页 |
| Chapter 1 Introduction | 第11-30页 |
| 1.1 Source of the research | 第11-12页 |
| 1.2 Research background and significance | 第12-25页 |
| 1.2.1 Plant cell wall | 第12-16页 |
| 1.2.2 Enzyme cellulase and xylanase | 第16-18页 |
| 1.2.3 The factor which effect to produce cellulase and xylanase of fungi | 第18-22页 |
| 1.2.4 Aspergillus niger | 第22-24页 |
| 1.2.5 Trichoderma viride | 第24-25页 |
| 1.3 Research Objective and purpose | 第25-28页 |
| 1.4 Scope of the research | 第28-30页 |
| Chapter 2 Material and Method | 第30-40页 |
| 2.1 Oil palm trunk OPT) pretreatment | 第30-31页 |
| 2.2 Strain preparation | 第31-33页 |
| 2.2.1 Spore suspension concentration preparation (1×10~8 spore/m L) | 第31-33页 |
| 2.3 Test antagonism of A. niger and T. viride | 第33页 |
| 2.4 Solid state fermentation(SSF) process | 第33-34页 |
| 2.5 Study the produce Cellulase and Xylanase activity of A. niger and T. Viride onOPT | 第34-35页 |
| 2.5.1 Study produce cellulase and xylanese efficiency of A. niger, T. viride and the mix culture of A. niger and T. viride in Oil Palm trunk tree powder(OPT) thoughtsubstrate fermentation by use distilled water for moisten substrate | 第34页 |
| 2.5.2 Study produce cellulase and xylanese efficiency of A. niger , T. viride and the mix culture of them in Oil Palm trunk tree powder(OPT) thought substratefermentation by moisten substrate with Mendel medium | 第34页 |
| 2.5.3 The suitable initial p H of the medium. | 第34-35页 |
| 2.5.4 The suitable temperature of microorganism for highest cellulase and xylanase production. | 第35页 |
| 2.5.5 The effect of size of substrate. | 第35页 |
| 2.6 Clude cellulase and Xylanase enzymes extraction | 第35-36页 |
| 2.7 Analysis method | 第36-39页 |
| 2.7.1 Analyze reducing sugar by 3,5-Dinitrosalicylic acid(DNS) Reagent. | 第36-37页 |
| 2.7.1.1. The chemical preparation | 第36页 |
| 2.7.1.2. The standard curve of glucose and xylose preparation | 第36-37页 |
| 2.7.2.Sample analysis | 第37页 |
| 2.7.3. Standard curve of reducing sugar analysis by DNS | 第37-39页 |
| 2.8 Statistic analysis | 第39-40页 |
| Chapter 3 Result and discussion | 第40-64页 |
| 3.1 The standard curve of glucose and xylose sugar | 第40-41页 |
| 3.2 Test antagonist of A. niger and T. viride on PDA agar | 第41-42页 |
| 3.3 Study produce cellulase and xylanese efficiency of A. niger , T. viride and the mix culture of them in Oil Palm trunk powder thought substrate fermentation withoutadded nutrient source | 第42-43页 |
| 3.4 Study produce cellulase and xylanese efficiency of A. niger , T. viride and the mix culture of them in Oil Palm trunk powder thought substrate fermentation by useMendel solution for generate final moisture | 第43-50页 |
| 3.5 The suitable initial p H of the substrate for produce cellulase and xylanase whichA. niger and T. viride can got highest production | 第50-55页 |
| 3.6 The suitable temperature of A. niger, T. viride and mix culture for highestcellulase and xylanase production | 第55-59页 |
| 3.7 Study effect of size of substrate for highest cellulase and xylanase production. 49Chapter 4 Conclusions | 第59-64页 |
| Chapter 4 Conclusion | 第64-66页 |
| 4.1 Test antagonist of A. niger and T. viride on PDA agar. | 第64页 |
| 4.2 Study produce cellulase and xylanese efficiency of A. niger , T. viride and the mix culture of A. niger and T. viride in Oil Palm trunk tree powder thought substrate fermentation without added nutrient source. | 第64页 |
| 4.3 Study produce cellulase and xylanese efficiency of A. niger , T. viride and the mix culture of A. niger and T. viride in Oil Palm trunk tree powder thought substrate fermentation by added nutrient source. | 第64页 |
| 4.4 The suitable initial pH of the substrate for produce cellulase and xylanase that can got highest production. | 第64-65页 |
| 4.5 The suitable temperature of A. niger, T.viride and mix culture for highest cellulase and xylanase production. | 第65页 |
| 4.6 Effect of size of substrate for highest cellulase and xylanase production. | 第65-66页 |
| Reference | 第66-71页 |
| ACKNOWLEDGEMENT | 第71页 |
