| acknowledgements | 第5-13页 |
| 摘要 | 第13-14页 |
| Abstract | 第14-15页 |
| Abbreviations | 第16-17页 |
| Chapter 1 Introduction | 第17-37页 |
| 1.1 Epidemiology of Japanese Encephalitis Virus | 第17-19页 |
| 1.2 History | 第19-21页 |
| 1.3 Geographic distribution of Japanese encephalitis virus | 第21-22页 |
| 1.4 Molecular Characterization of Japanese Encephalitis Virus | 第22-24页 |
| 1.5 Mosquito Vectors of Japanese Encephalitis Virus | 第24-26页 |
| 1.6 Japanese encephalitis Infection in vertebrates | 第26-28页 |
| 1.6.1 Infection in human | 第26-27页 |
| 1.6.2 Infection in pigs | 第27-28页 |
| 1.6.3 Infection in other animals | 第28页 |
| 1.7 Toll-like Receptors 7 activate innate immunity | 第28-33页 |
| 1.8 Activation of Innate immunity and adaptive immunity | 第33-35页 |
| 1.9 Surface molecule | 第35-37页 |
| Chapter 2 Materials and Methods | 第37-45页 |
| 2.1 Mice | 第37页 |
| 2.2 Virus and Cells | 第37页 |
| 2.3 Bone marrow preparation | 第37-38页 |
| 2.4 Bone marrow cell culture with GM-CSF | 第38页 |
| 2.5 JEV-P3 infection of bmDCs | 第38-39页 |
| 2.6 JEV-P3 inoculums in mice | 第39页 |
| 2.7 Quantitative real-time RT-PCR to detect viral load and cytokine expression | 第39-40页 |
| 2.8 Preparation of RNA Sample | 第40页 |
| 2.9 Preparation of cDNA for Quantitative real-time RT-PCR | 第40-41页 |
| 2.10 Preparation of Plates for Quantitative real-time RT-PCR | 第41页 |
| 2.11 Flow cytometry | 第41-42页 |
| 2.12 Immunohistochemistry | 第42页 |
| 2.13 Hematoxylin eosin staining (HE staining) | 第42-43页 |
| 2.14 Immunofluorescence assay | 第43-44页 |
| Statistical Analysis | 第44-45页 |
| Chapter 3 Results | 第45-68页 |
| 3.1 In-vitro study | 第45-52页 |
| 3.1.1 TLR7 is up-regulated on spleen after JEV infection | 第45-46页 |
| 3.1.2 Expression of TLR7 on brain in C57BL/6 mice post JEV infection | 第46-47页 |
| 3.1.3 TLR7 is up-regulated on bmDC after JEV infection | 第47-48页 |
| 3.1.4 Expression of CD80 at 48 H and 72H post JEV infection | 第48-49页 |
| 3.1.5 Expression of CD86 at 48 H and 72H post JEV infection | 第49-50页 |
| 3.1.6 Expression of CD274 at 48 H and 72H post JEV infection | 第50-51页 |
| 3.1.7 Expression of CD273 at 48H and 72H post JEV infection | 第51-52页 |
| 3.2 In-vivo study | 第52-62页 |
| 3.2.1 TLR7 deficiency did not change survivorship and body weight | 第52-53页 |
| 3.2.2 Increased viral load in the brain in TLR7 deficient mice | 第53-54页 |
| 3.2.3 Response of TLR8 in TLR7 deficient mice post-JEV infection | 第54-55页 |
| 3.2.4 Response of TLR8 in TLR7 deficient mice | 第55-56页 |
| 3.2.5 Histopathological changes of brain tissue in TLR7 deficient mice withoutJEV infection | 第56-57页 |
| 3.2.6 Pathological changes on brain tissue after JEV infection in TLR7 deficientmice without disease signs | 第57-58页 |
| 3.2.7 Pathological changes on brain tissue in TLR7 deficient mice after JEVinfection with disease signs | 第58-59页 |
| 3.2.8 Detection of TLR8 and JEV E protein in brain tissue of TLR7 deficient miceafter JEV-P3 strain without disease signs | 第59-60页 |
| 3.2.9 Detection of TLR8 and JEV-E protein in brain tissue of TLR7 deficient micepost JEV infection with disease signs | 第60-61页 |
| 3.2.10 Detection of TLR8 on brain tissue in TLR7 deficient mice post JEVinfection | 第61-62页 |
| 3.3 Immunology study | 第62-68页 |
| 3.3.1 Responses of DC, and pDC on spleen in TLR7 deficient mice | 第62-63页 |
| 3.3.2 Responses of DC, and pDC on blood in TLR7 deficient mice | 第63-65页 |
| 3.3.3 Response of CD4 and CD8 +T subsets cell on spleen in TLR7 deficient mice | 第65-66页 |
| 3.3.4 Response of CD4 and CD8 +T subsets cell on blood in TLR7 deficient mice | 第66-67页 |
| 3.3.5 Response of Treg subset cell in TLR7 deficient mice | 第67-68页 |
| Chapter 4 Discussion | 第68-75页 |
| 4.1 TLR7 is superior as compare to other TLRs | 第69页 |
| 4.2 Role of TLR8 against JEV in TLR7~(-/-) mice | 第69-71页 |
| 4.3 CD80, CD 86 and pDC Response against JEV infection | 第71-72页 |
| 4.4 T cell response in TLR7~(-/-) mice against JEV infection | 第72-73页 |
| 4.5 Response of Treg subset cells in TLR7~(-/-) mice post JEV infection | 第73-75页 |
| Conclusion | 第75-77页 |
| References | 第77-91页 |
| List of Publications | 第91页 |
