| 附件 | 第4-5页 |
| 摘要 | 第5-6页 |
| Abstract | 第6页 |
| Abbreviations | 第11-12页 |
| 1 Introduction | 第12-31页 |
| 1.1 General Information | 第12-14页 |
| 1.2 Literature Review | 第14-31页 |
| 1.2.1 Elicitors | 第14-16页 |
| 1.2.2 Protein Elicitor | 第16-18页 |
| 1.2.3 Rice Blast fungus | 第18-22页 |
| 1.2.4 Defense signaling pathway in plants | 第22-27页 |
| 1.2.4.1 Salicylic-acid-mediated defenses | 第23页 |
| 1.2.4.2 Jasmonic-acid-dependent defenses | 第23页 |
| 1.2.4.3 Ethylene-dependent responses | 第23-24页 |
| 1.2.4.4 cross talk in defense signaling | 第24-27页 |
| 1.2.5 Rice Growth Stages | 第27-29页 |
| 1.2.5.1 Germination | 第27-28页 |
| 1.2.5.2 Vegetative | 第28页 |
| 1.2.5.3 Reproductive | 第28页 |
| 1.2.5.4 Ripening | 第28-29页 |
| 1.2.6 Aim and Objectives | 第29-31页 |
| 1.2.6.1 Technology Roadmap | 第30-31页 |
| 2 Induce resistance and growth enhancement of Hrip1 protein elicitor from alternaria tenuissima in Rice | 第31-42页 |
| 2.1 Introduction | 第31-32页 |
| 2.2 Material and Methods | 第32-35页 |
| 2.2.1 Protein Expression and Purification | 第32-33页 |
| 2.2.2 Pathogen and plant cultivation | 第33页 |
| 2.2.3 Bioassay for Hrip1-induced disease resistance in rice | 第33页 |
| 2.2.4 Analysis of the expression of pathogenesis-related genes induced by Hrip1 using Quantitative rea | 第33-34页 |
| 2.2.5 Measurement of rice plant height,root length and number of seeds germination | 第34-35页 |
| 2.2.5.1 Measurement of rice plant Height in pot | 第34-35页 |
| 2.2.6 Statistical analysis | 第35页 |
| 2.3 Result and Discussion | 第35-42页 |
| 2.3.1 expression and purification of the elicitor protein | 第35-36页 |
| 2.3.2 growth enhancement of rice seedling by Hripl protein elicitor | 第36-38页 |
| 2.3.2.1 Growth enhancement of rice plant was growing in soil by Hrip1 | 第37-38页 |
| 2.3.3 Induction of resistance to rice blast by Hrip1 | 第38-40页 |
| 2.3.4 The defense signaling network depends on Hrip1 in rice plants | 第40-42页 |
| 3 Induction of resistance and promotion of Growth of Peatl elicitor from altemaria tenuissima in Rice | 第42-49页 |
| 3.1 Introduction | 第42-43页 |
| 3.2 Material and Methods | 第43-44页 |
| 3.2.1 Expression and purification of elicitor PeaT1 | 第43页 |
| 3.2.2 Pathogen and plant cultivation | 第43页 |
| 3.2.3 Bioassay for PeaT1-induced disease resistance in rice | 第43页 |
| 3.2.4 Measurement of rice plant height,root length and number of seeds germination | 第43-44页 |
| 3.2.4.1 Measurement of rice plant Height in pot | 第44页 |
| 3.3 Result and Discussion | 第44-49页 |
| 3.3.1 Purification of elicitor PeaT1 | 第44-45页 |
| 3.3.2 growth enhancement of rice seedling by PeaT1 protein elicitor | 第45-47页 |
| 3.3.2.1 Growth enhancement of rice plant was growing in soil by PeaT1 | 第46-47页 |
| 3.3.3 Induce resistance against rice blast by Peatl protein elicitor | 第47-49页 |
| 4 Induce resistance against rice blast fungus and growth enhancement in rice by PevD1 | 第49-56页 |
| 4.1 Introduction | 第49-50页 |
| 4.2 Material and methods | 第50-51页 |
| 4.2.1 Expression and purification of PevD1 elicitor | 第50页 |
| 4.2.2 Pathogen and plant cultivation | 第50页 |
| 4.2.3 Bioassay for PevD1-induced disease resistance in rice | 第50-51页 |
| 4.2.4 Measurement of rice plant height,root length and number of seeds germination | 第51页 |
| 4.2.5 Measurement of rice plant Height in pot | 第51页 |
| 4.3 Result and Discussion | 第51-56页 |
| 4.3.1 Purification of elicitor | 第51-52页 |
| 4.3.2 growth enhancement of rice seedling by PevD1 protein elicitor | 第52-53页 |
| 4.3.2.1 Growth enhancement of rice plant was growing in soil by PevD1 | 第53页 |
| 4.3.3 Induce resistance against rice blast by PevD1 protein elicitor | 第53-56页 |
| 5 Study on Growth enhancement in rice by Mohrip1 elicitor and Comparison of MoHrip1 Protein elicitor withHrip1,PeaT1 and PevD1 in growth enhancement and Resistance to rice blast | 第56-64页 |
| 5.1 Introduction | 第56-57页 |
| 5.2 Material and Methods | 第57-58页 |
| 5.2.1 Protein preparation | 第57页 |
| 5.2.2 plant cultivation | 第57页 |
| 5.2.3 Measurement of rice plant height,root length and number of seeds germination | 第57-58页 |
| 5.2.3.1 Measurement of rice plant Height in pot | 第58页 |
| 5.3 Result and Discussion | 第58-64页 |
| 5.3.1 expression and purification of the elicitor protein | 第58-59页 |
| 5.3.2 growth enhancement of rice seedling by MoHrip1 protein elicitor | 第59-60页 |
| 5.3.2.1 Growth enhancement of soil-grown rice by MoHrip1 treatment | 第60页 |
| 5.3.3 Comparison of Protein elicitors in growth enhancement of rice seedling,number of seed germination and soil grown rice plant height | 第60-62页 |
| 5.3.4 Comparision of Protein elicitors in growth enhanccement of soil grown rice | 第62页 |
| 5.3.5 Comparison of Protein elicitors in induce resistance against rice blast fungus in rice plants | 第62-64页 |
| Conclusion | 第64-66页 |
| References | 第66-78页 |
| ACKNOWLEDGEMENTS | 第78-79页 |
| CV | 第79-80页 |
| PUBLICATION | 第80页 |
