| DEDICATION | 第3-4页 |
| ACKNOWLEDGEMENTS | 第4-5页 |
| ABSTRACT | 第5-7页 |
| Abbreviations | 第12-13页 |
| CHAPTER 1 INTRODUCTION | 第13-27页 |
| 1.1 Introduction | 第13-18页 |
| 1.2 Literature review | 第18-27页 |
| 1.2.1 Soil phosphorus | 第19-20页 |
| 1.2.2 Applied phosphate | 第20-21页 |
| 1.2.3 Phosphorus solubilizing bacte ria PSB | 第21-22页 |
| 1.2.4 Mechanisms of phosphorus solubilization | 第22-24页 |
| 1.2.5 Bacterial diversity | 第24-25页 |
| 1.2.6 Role of PSB in crop productivity | 第25-27页 |
| CHAPTER 2 ISOLATION.AND CHARACTERATION PHOSPHORUS SOLUBILIZING BACTERIA | 第27-38页 |
| 2.1 Material and methods | 第27页 |
| 2.1.1 Soil samples collection | 第27页 |
| 2.1.2 Sterilization of glassware | 第27页 |
| 2.1.3 Measurable of soil pH | 第27页 |
| 2.2 Determination of soil soluble phosphorus | 第27-29页 |
| 2.2.1 Extraction and reagents | 第28页 |
| 2.2.2 Standard series of Phosphorus | 第28-29页 |
| 2.2.3 Procedure | 第29页 |
| 2.2.4 Calculation | 第29页 |
| 2.3 Isolation of bacteria from soil sample | 第29-30页 |
| 2.3.1 Screening of Phosphorus solubilizing bacteria(PSB) | 第29-30页 |
| 2.3.2 Storage of bacterial isolate in glycerol | 第30页 |
| 2.3.3 Characterization of phosphorus solubilizing bacteria | 第30页 |
| 2.3.4 Gram staining | 第30页 |
| 2.4 Molecular characterization of bacteria isolate | 第30-34页 |
| 2.4.1 Isolation of genomic DNA from bacteria isolate | 第31页 |
| 2.4.2 Electrophoresis of DNA on agarose gels | 第31-32页 |
| 2.4.3 DNA purification | 第32页 |
| 2.4.4 Ethidium bromide fluorescent quantification | 第32页 |
| 2.4.5 Amplification of 16S rDNA | 第32-34页 |
| 2.4.6 Purification of PCR products | 第34页 |
| 2.4.7 DNA sequence analysis | 第34页 |
| 2.5 Results and discussion | 第34-37页 |
| 2.5.1 General characteristics of the isolate | 第34-36页 |
| 2.5.2 Identification of phosphorus solubilizing bacterium strain | 第36-37页 |
| 2.6 Summary | 第37-38页 |
| CHAPTER 3 IN VITRO EXPERIMENTTO TEST THE SELECTED ISOLATE AS PHOSPHORUS SOLUBILIZING BACTERIA | 第38-44页 |
| 3.1 Material and methods | 第38页 |
| 3.2 Results | 第38-42页 |
| 3.2.1 Effect of inoculation with selected bacterial isolate on pH decreased under in vitro condition26 | 第38-40页 |
| 3.2.2 Determination of phosphorus solubilization in liquid culture | 第40-42页 |
| 3.3 Discussion | 第42-43页 |
| 3.4 Summary | 第43-44页 |
| CHAPTER 4 EFFECT OF BACTERIA INOCULANT AS PSB ON TOMATO AND WHEAT PLANT WITH DIFFERENTLEVELS OF PHOSPHATE FERTILIZER IN POTS EXPERIMENT | 第44-65页 |
| 4.1 Material and methods | 第44-46页 |
| 4.1.1 Preparation of bacterial inoculant | 第44页 |
| 4.1.2 Cultivation | 第44页 |
| 4.1.3 Collection | 第44页 |
| 4.1.4 Soil sterilization | 第44-45页 |
| 4.1.5 Soil preparation | 第45页 |
| 4.1.6 Application | 第45页 |
| 4.1.7 Application of selected isolate as phosphorus solubilizing bacteria with three levels of super phosphate in pots experiment | 第45页 |
| 4.1.8 Experiment irrigation | 第45-46页 |
| 4.2 Analysis plant dry weight | 第46-48页 |
| 4.2.1 Acid Extraction for Total Phosphorus | 第46页 |
| 4.2.2 Procedure | 第46-47页 |
| 4.2.3 Total phosphorus in plant samples | 第47页 |
| 4.2.4 Procedure | 第47-48页 |
| 4.2.5 Calculation | 第48页 |
| 4.2.6 Parameters analysis | 第48页 |
| 4.3 Results | 第48-58页 |
| 4.3.1 Effect of inoculation with selected PSB on plant height at different super phosphate fertilizer levels in a pot experiment | 第48-51页 |
| 4.3.2 Effect of inoculation with selected PSB on plant stem diameter at different super phosphate fertilizer levels in a pot experiment | 第51-53页 |
| 4.3.3 Effect of inoculation with selected PSB on plant leaf area, fresh weight, and dry weight at different super phosphate fertilizer levels in a pot experiment | 第53-56页 |
| 4.3.4 Effect of inoculation with selected PSB on soil available phosphorus in soil in a pot experiment | 第56-57页 |
| 4.3.5 Effect of inoculation with selected PSB on total phosphorus content in the plant material at different super phosphate fertilizer levels in a pot experiment | 第57-58页 |
| 4.4 Discussion | 第58-59页 |
| 4.5 Summary | 第59-60页 |
| 4.6 Effect of inoculation with PSB on wheat plant height at different super phosphate fertilizer levels ina pots experiment | 第60-63页 |
| 4.6.1 Effect of PSB inoculation at different super phosphate fertillizer levels on wheat stem diameter in pots experiment | 第61页 |
| 4.6.2 Effect of PSB inoculant on wheat plant,leaf area,dry weight,available phosphorus in soil, and plant material at different super phosphate fertilizer levels in pots experiment | 第61-63页 |
| 4.7 Discussion | 第63页 |
| 4.8 Summary | 第63-65页 |
| CHAPTER 5 EFFECT OF BACTERIAL ISOLATE AS PSB ON TOMATO PLANT WITHOUT PHOSPHATE FERTILIZER IN POTS EXPERIMENT | 第65-79页 |
| 5.1 Material and methods | 第65-66页 |
| 5.1.1 Irrigation | 第65-66页 |
| 5.2 Results | 第66-76页 |
| 5.2.1 Effect of bacterial inoculation with different amount of cells on tomato plant height in pots experiment | 第66-69页 |
| 5.2.2 Effect of bacterial inoculation with different amount of cells on plant stem diameter in pots experiment | 第69-72页 |
| 5.2.3 Effect of bacterial inoculation with different amount of cells on plant leaf area,fresh weight, and dry weight in pots experiment | 第72-74页 |
| 5.2.4 Effect of bacte rial inoculation on available phosphorus in the soil in pots experiment | 第74-75页 |
| 5.2.5 Effect of bacterial inoculation with different amount of cells on phosphorus uptake by plant in pots experiment | 第75-76页 |
| 5.3 Discussion | 第76-77页 |
| 5.4 Summary | 第77-79页 |
| CHAPTER 6 CONCLUSION | 第79-82页 |
| 6.1 Conclusion | 第79-82页 |
| REFERENCES | 第82-95页 |
| Appendix | 第95-100页 |
