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C3植物籼、粳水稻光合作用相关基因及其与DNA甲基化关系的研究

摘要第6-8页
ABSTRACT第8-9页
LIST OF ABBREVIATION第17-19页
Chapter1 Introduction第19-38页
    1.1 Rice(Oryza sativa)第19-20页
        1.1.1 Indica and japonica rice第19-20页
    1.2 Rice and future challenges第20页
    1.3 Photosynthesis第20-24页
        1.3.1 C3 Photosynthesis第21-23页
        1.3.2 C4 Photosynthesis第23-24页
        1.3.3 The site of photosynthesis in plants第24页
    1.4 Epigenetic regulation of photosynthesis in rice第24-26页
    1.5 DNA methylation第26-31页
        1.5.1 DNA Adenine Methylation(6mA)In Plants第27-29页
        1.5.2 DNA Cytosine Methylation(5mC)第29-31页
    1.6 RNA directed DNA methylation (RdDM) pathway第31-32页
    1.7 DNA demethylation第32页
    1.8 DNA methyltransferases and their functions第32-33页
    1.9 DNA demethylases and their functions第33-34页
    1.10 Methods for detecting DNA Methylation第34-36页
    1.11 Significance and objectives of the study第36-38页
Chapter2 Evaluation of leaf color,grain yield,gas exchange measurement,chlorophyll florescence and photosynthetic pigments in indica and japonica rice第38-52页
    2.1 Summary第38页
    2.2 Introduction第38-40页
    2.3 Material and Methods第40-42页
        2.3.1 Plant material and growth conditions第40页
        2.3.2 Chlorophyll Contents第40-41页
        2.3.3 Gas Exchange measurements第41页
        2.3.4 Chlorophyll florescence measurements第41页
        2.3.5 Assessment of yield and other related traits第41-42页
        2.3.6 Statistical analysis第42页
    2.4 Results第42-49页
        2.4.1 Assessment of Leaf color variation第42-43页
        2.4.2 Assessment of yield and other related traits第43-44页
        2.4.3 Flag leaf chlorophyll contents and chlorophyll a/b ratio第44-46页
        2.4.4 Variation in photosynthesis rate and other gas-exchange traits第46-48页
        2.4.5 Variation in chlorophyll florescence parameters第48-49页
    2.5 Discussion第49-52页
        2.5.1 Leaf color第49-50页
        2.5.2 Leaf chlorophyll contents第50页
        2.5.3 Gas exchange第50-51页
        2.5.4 Chlorophyll Florescence第51-52页
Chapter3 Characterization of key photosynthesis related genes in Rice第52-69页
    3.1 Summary第52页
    3.2 Introduction第52-53页
    3.3 Material and Methods第53-57页
        3.3.1 Plant materials and growth conditions第53页
        3.3.2 Gene identification and sequence analysis第53-55页
        3.3.3 RNA isolation第55页
        3.3.4 Quantification of RNA on Nano-drop spectrophotometer第55-56页
        3.3.5 cDNA synthesis第56页
        3.3.6 Quantitative real time PCR(qRT-PCR)第56-57页
    3.4 Results第57-65页
        3.4.1 Expression pattern of key photosynthetic genes in rice第57-58页
        3.4.2 Expression Pattern of PEPC and PEPC第58页
        3.4.3 Expression Pattern of NADP-ME and NADP-MDH第58-59页
        3.4.4 Expression Pattern of PPDK and PPDK-RP第59-60页
        3.4.5 Expression Pattern of PP and AK第60页
        3.4.6 Expression Pattern of NAD-ME and MEP3A第60-61页
        3.4.7 Expression Pattern of TPT and PPT第61-62页
        3.4.8 Expression Pattern of PCK and CA第62页
        3.4.9 Expression Pattern of ASPAT and GLK第62-63页
        3.4.10 Expression Pattern of Phytochromes PHYA and PHYB第63-64页
        3.4.11 Expression Pattern of RBCS and RBCACT第64页
        3.4.12 Expression Pattern of AMT1 and DiT第64-65页
    3.5 Discussion第65-69页
        3.5.1 Regulation of key photosynthesis pathway genes第65-67页
        3.5.2 Transporters and translocators in photosynthesis pathway第67-69页
Chapter4 Estimation of global and photosynthetic gene specific associated methylation levels in Rice第69-85页
    4.1 Summary第69页
    4.2 Introduction第69-71页
    4.3 Material and methods第71-77页
        4.3.1 Plant materials and growth conditions第71-72页
        4.3.2 DNA extraction第72页
        4.3.3 Dot blot assay第72-74页
        4.3.4 Liquid Chromatography-Mass Spectrometry(LC-MS/MS)assay第74页
        4.3.5 Bisulfite conversion and sequencing第74-77页
        4.3.6 Data analysis第77页
    4.4 Results第77-83页
        4.4.1 Dot Blot analysis of DNA modifications第77-79页
        4.4.2 LC-MS/MS analysis of DNA modifications第79-83页
    4.5 Discussions第83-85页
        4.5.1 DNA modifications are dynamic in plants第83-85页
Chapter5 Conclusion第85-87页
REFERENCES第87-104页
ACKNOWLEDGEMENT第104-105页
RESUME第105-106页

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