| ACKNOWLEDGEMENTS | 第10-18页 |
| Abstract | 第18-19页 |
| 摘要 | 第20-22页 |
| Abbreviations | 第22-23页 |
| Chapter 1 Literature review | 第23-30页 |
| 1.1 Background | 第23页 |
| 1.2 Effects of Co~(2+) on plant growth | 第23-24页 |
| 1.3 Effect of Co~(2+) on photosynthetic pigments and hill reaction | 第24-25页 |
| 1.4 Effect of Co~(2+) on carbonylated protein accumulation,DNA fragmentation,and induction of DNase activity | 第25-26页 |
| 1.5 ROS and modulation of antioxidant enzymes under Co~(2+) stress | 第26-27页 |
| 1.6 Effect of Co~(2+) stress on Co~(2+) and some nutrient contents | 第27-28页 |
| 1.7 Effect of Co~(2+) stress on Co~(2+) on subcellular distribution and chemical forms of Co~(2+) | 第28-29页 |
| 1.8 Research objectives | 第29-30页 |
| Chapter 2 Genotypic difference of cobalt stress resistance in barley | 第30-42页 |
| 2.1 Materials and Methods | 第30-32页 |
| 2.1.1 Plant material and hydroponic culture | 第30-31页 |
| 2.1.2 Plant sampling and measurement | 第31-32页 |
| 2.1.3 Cobalt content | 第32页 |
| 2.1.4 Translocation factor of cobalt | 第32页 |
| 2.1.5 Statistic analysis | 第32页 |
| 2.2 Results | 第32-40页 |
| 2.2.1 Preliminary screening selection | 第32-35页 |
| 2.2.2 Second screening experiment | 第35-40页 |
| 2.3 Discussion | 第40-42页 |
| Chapter 3 The Effect of Co~(2+) Stress on Growth and Physiological Traits and its Association with Co Accumulation in Barley Genotypes Differing in Co~(2+) Resistance | 第42-60页 |
| 3.1 Materials and Method | 第43-47页 |
| 3.1.1 Plant material and growth condition | 第43-44页 |
| 3.1.2 Plant biomass and physiological parameters | 第44页 |
| 3.1.3 Proline content | 第44-45页 |
| 3.1.4 Biochemical analysis | 第45-46页 |
| 3.1.5 Lipid peroxidation and reactive oxygen species | 第46-47页 |
| 3.1.6 Element content | 第47页 |
| 3.1.7 Statistical analysis | 第47页 |
| 3.2 Results | 第47-55页 |
| 3.2.1 The effect of Co~(2+) stress on plant growth | 第47-49页 |
| 3.2.2 Effects of Co~(2+) stress on chlorophyll content and photosynthetic parameters | 第49-50页 |
| 3.2.3 Effects of Co~(2+) stress on the contents of proline,MDA,hydrogen peroxide and superoxide radicals | 第50-51页 |
| 3.2.4 Effects of Co~(2+) on total soluble protein (TSP) content | 第51-52页 |
| 3.2.5 Effects of Co~(2+) on antioxidant enzyme activities | 第52-54页 |
| 3.2.6 Effect of Co~(2+) stress on Co~(2+) and some nutrient contents | 第54-55页 |
| 3.3 Discussion | 第55-60页 |
| Chapter 4 Subcellular distribution and chemical forms of Co~(2+) in three Barley Genotypes under different Co~(2+) levels | 第60-76页 |
| 4.1 Materials and Methods | 第62-65页 |
| 4.1.1 Plant material and hydroponic culture | 第62页 |
| 4.1.2 Treatments and sampling | 第62-63页 |
| 4.1.3 Cobalt content | 第63页 |
| 4.1.4 Separation and measurement of subcellular fractions | 第63-64页 |
| 4.1.5 Separation and measurement of chemical forms | 第64页 |
| 4.1.6 Statistical analysis | 第64-65页 |
| 4.2 Results | 第65-72页 |
| 4.2.1 The effect of Co~(2+) stress on plant growth and cobalt concentration | 第65-66页 |
| 4.2.2 Subcellular distribution of Co~(2+) | 第66-69页 |
| 4.2.3 Chemical form of Co~(2+) | 第69-72页 |
| 4.3 Discussion | 第72-76页 |
| Chapter 5 Conclusion and prospective | 第76-78页 |
| References | 第78-87页 |
| List of publications | 第87页 |
