| 中文摘要 | 第10-13页 |
| Abstract | 第13-15页 |
| Abbreviations | 第16-19页 |
| Chapter 1 Literature review | 第19-41页 |
| 1.1 Herbicide mode of action | 第19-31页 |
| 1.2 Herbicide resistance | 第31-33页 |
| 1.3 Bioherbice | 第33-39页 |
| 1.4 Aims of the present study | 第39-41页 |
| Chapter 2 Primary Study of the action site of TeA | 第41-61页 |
| 中文摘要 | 第41-42页 |
| Abstract | 第42页 |
| 2.1 Introduction | 第42-44页 |
| 2.2 Materials and methods | 第44-49页 |
| 2.3 Results and analysis | 第49-57页 |
| 2.3.1 Effect of TeA on oxygen evolution in photosynthesis and Chlorophyll a and b of leaf of E. adenophorum | 第49-50页 |
| 2.3.2 Effect of TeA on two photosystems and membrane protein in thylakoids | 第50-52页 |
| 2.3.3 Effect of TeA on total soluble protein and RuBPCase of E. adenophorum | 第52-53页 |
| 2.3.4 Effect of TeA on chlorophyll a fluorescence of leaves of E. adenophorum | 第53-56页 |
| 2.3.5 Effect of TeA on the chlorophyll fluorescence kinetics of PSII pellets of E. adenophorum | 第56页 |
| 2.3.6 Effect of TeA on ATPase activity of thylakoids of E. adenophorum | 第56-57页 |
| 2.3.7 Effect of TeA on mitochondria | 第57页 |
| 2.4 Discussion | 第57-61页 |
| Chapter 3 Study of precise action site of TeA in photosystem II | 第61-98页 |
| 中文摘要 | 第61-62页 |
| Abstract | 第62页 |
| 3.1 Introduction | 第62-64页 |
| 3.2 Materials and methods | 第64-72页 |
| 3.3 Results and analysis | 第72-90页 |
| 3.3.1 Effect of TeA on PSII electron transport activity of thylakoid membranes | 第72-73页 |
| 3.3.2 Effect of TeA on fluorescence rise transient kinetics OJIP curves | 第73-75页 |
| 3.3.3 Effect of TeA on behaviour of PSII: the JIP test | 第75-78页 |
| 3.3.4 Effect of TeA on PSII reaction centers | 第78-83页 |
| 3.3.5 Effect of TeA on turn over Q_A reducing events | 第83-86页 |
| 3.3.6 Effect of TeA on the FNR system and the reduction of NADP~+ | 第86页 |
| 3.3.7 Effect of TeA on the pipeline models | 第86-89页 |
| 3.3.8 Binding competition between TeA and [~(14)C]atrazine | 第89-90页 |
| 3.4 Discussion | 第90-98页 |
| Chapter 4 Identification of TeA binding in Qs-site of photosystem II | 第98-123页 |
| 中文摘要 | 第98-99页 |
| Abstract | 第99-100页 |
| 4.1 Introduction | 第100-101页 |
| 4.2 Materials and methods | 第101-105页 |
| 4.3 Results and analysis | 第105-116页 |
| 4.3.1 Effect of TeA on O_2 evolution rate of PSII of thylakoids | 第105-106页 |
| 4.3.2 Effect of TeA on chlorophyll fluorescence induction rise kinetics of C. reinhardtii | 第106-108页 |
| 4.3.3 JIP-test: effect of TeA on activity of PSII reaction center | 第108-109页 |
| 4.3.4 Competitive binding between TeA and atrazine assay | 第109-110页 |
| 4.3.5 Effect of TeA on photoaffinity labeling protein of atrazine | 第110页 |
| 4.3.6 Thylakoids of wild-type and mutants D1 of C. reinhardtii sensitivity to TeA assay | 第110-112页 |
| 4.3.7 Effect of TeA on some parameters from JIP-test of wild-type and mutant D1-Gly256Asp of C. reinhardtii | 第112-113页 |
| 4.3.8 Competitive binding of TeA by thylakoids of wild type and mutant D1-Gly256Asp of C. reinhardtii | 第113-115页 |
| 4.3.9 Proposed modeling: TeA binding sites | 第115页 |
| 4.3.10 Analysis of biology activity of TeA and its analogues | 第115-116页 |
| 4.4 Discussion | 第116-123页 |
| Chapter 5 Mechanism of TeA-caused cell death of Eupatorium adenophorum through production of reactive oxygen species | 第123-165页 |
| 中文摘要 | 第123-125页 |
| Abstract | 第125页 |
| 5.1 Introduction | 第125-130页 |
| 5.2 Materials and methods | 第130-137页 |
| 5.3 Results and analysis | 第137-158页 |
| 5.3.1 Timing and pattern of TeA-induced damage in leaves | 第137-138页 |
| 5.3.2 Histochemical location determines TeA-induced oxidative burst | 第138-143页 |
| 5.3.3 Dichlorofluorescein measures TeA-induced ROS production in mesophyll cells and epidermal peels and protoplasts | 第143-145页 |
| 5.3.4 Cytochemical location confirms cellular sources of TeA-induced H_2O_2 generation | 第145-147页 |
| 5.3.5 EPR detects the species of TeA-induced ROS in thylakoids | 第147-150页 |
| 5.3.6 TeA incubation causes damage to mesophyll cells | 第150-155页 |
| 5.3.7 ROS induced by TeA cause damage in nucleus | 第155-158页 |
| 5.4 Discussion | 第158-165页 |
| 全文结论与讨论 | 第165-171页 |
| References cited | 第171-198页 |
| 致谢 | 第198-199页 |
| 攻读博士学位期间撰写及发表论文、发明专利和参加科研项目目录 | 第199-201页 |
| 附表 | 第201-207页 |
