| Abstract | 第1-11页 |
| 中文摘要 | 第11-12页 |
| Chapter 1.Introduction | 第12-17页 |
| ·Tumor Metastasis and MMPs | 第12-14页 |
| ·Gangliosides | 第14页 |
| ·Phosphoinositide 3-kinase/AKT pathway | 第14-15页 |
| ·MAPKs | 第15页 |
| ·Caveolae | 第15-16页 |
| ·Summary | 第16-17页 |
| Chapter 2.Meterials and methods | 第17-22页 |
| ·Cell lines and cell culture | 第17页 |
| ·Chemicals | 第17-18页 |
| ·RNA extraction and semi-quantitatively RT-PCR | 第18-19页 |
| ·Gelatin zymography | 第19-20页 |
| ·Ganglioside extraction and HPTLC | 第20页 |
| ·Western blotting analysis | 第20-21页 |
| ·Stable transfection and monoclone | 第21-22页 |
| Chapter 3.Augment MMPs expression in st3gal5 siRNA and st3gal2 siRNA transfected Lewis cells | 第22-25页 |
| ·Increased MMPs expression in st3gal5 siRNA stable transfected Lewis cells | 第22-24页 |
| ·Augment MMPs expression in st3gal2 siRNA by stable transfection | 第24-25页 |
| Chapter 4.Diverse effect of ganglioside GD1a on MMP-9 regulation in Different Cell lines | 第25-32页 |
| ·Different effect of ganglioside GD1a on MMP-9 expression in different cells on transcriptional level | 第25-28页 |
| ·Similar effect of ganglioside GD1a on MMP-2 expression in different type of cells | 第28-30页 |
| ·GD1a in culture medium decreased MMP-9 but not MMP-2 expression by gelatin zymography | 第30-32页 |
| Chapter 5.The differences between B16 cells and Lewis cells | 第32-47页 |
| ·Compared with Lewis cells,B16 cells did not contain GD1a and MMPs secreation was less. | 第32-33页 |
| ·Some GD1a related singnals expression were examined in B16 cells and Lewis cells | 第33-38页 |
| ·GD1a regulated MMP-9 expression through different MAPK pathways between Lewis cells and B16 cells | 第38-40页 |
| ·TNF-α regulate MMP-9 expression through different MAPK pathways between Lewis cells and B16 cells | 第40-42页 |
| ·MβCD and Nystatin for involvement of caveolae in GD1a signals in B16 cells but not in Lewis cells | 第42-44页 |
| ·Akt is used as GD1a signal pathway both in B16 cells and Lewis cells | 第44-47页 |
| Chapter 6.Conclusions and discussion | 第47-49页 |
| References | 第49-52页 |
| Published paper | 第52-53页 |
| Acknowledgements | 第53页 |
