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拟南芥GOLDEN 2-LIKE转录因子参与调控ABA应答基因表达机制的研究

Abstract第4-7页
摘要第8-12页
Introduction第12-29页
    1.1.Germination第12-13页
    1.2.Abiotic Stress第13页
    1.3.Phytohormone第13-14页
    1.4.Abscisic Acid(ABA)第14-16页
    1.5.ABA has a Key Role in Germination第16-17页
    1.6.Transcription Factors ABI5 and ABI3第17-18页
    1.7.Abiotic Stress Particularly Drought Stress Affect Root Growth and Germination Process第18-20页
    1.8.WRKY Transcription Factor第20-23页
    1.9.Transcription Factor WRKY40第23-24页
    1.10.GOLDEN2-like Transcription Factor GLK第24-28页
    1.11.Transcription Factor GLK1/2 Involve in Germination第28-29页
Material and Methods第29-38页
    2.1.Plant Materials and Growth Conditions第29页
    2.2.Construction of Plasmids and Generation of Transgenic Plants第29-32页
    2.3.Assays of Sensitivity to ABA and Osmotic Stress第32页
    2.4.RNA-sequence analysis第32-34页
    2.5.Protoplast Isolation,Transfection,and GUS Activity Assay第34-35页
    2.6.Induced Transgene Expression in Stably Transformed Arabidopsis Plants第35页
    2.7.Transient Dual-luciferase Reporter System第35页
    2.8.Chromatin Immunoprecipitation Assays第35-36页
    2.9.Pull-down Assay and EMSA第36页
    2.10.DAB and NBT Staining Assay第36-37页
    2.11.Drought Tolerance Analysis第37页
    2.12.Seed Germination and Seedling Establishment Assays第37-38页
Results第38-67页
    3.1.A glk1 glk2 Double Mutant Displays Increased ABA-sensitivity During Seed Germination,Seedling Growth,and Induced Seed Dormancy第38-42页
    3.2.GLK1 and GLK2 Modulate ABA-mediated Regulation of Gene Expression第42-46页
    3.3.GLK1 and GLK2 Negatively Control the Osmotic and Dehydration Stress Responses第46-51页
    3.4.GLK1/2 specifically Activate the Expression of Some Essential ABA-responsive Genes第51-53页
    3.5.GLK1/2 Control the Expression of WRKY40 via Direct Binding at the Consensus Sequence Located at the Promoter Region第53-57页
    3.6.GLK1/2 and WRKY40 Share Common Target Genes in Response to ABA第57-59页
    3.7.GLK1/2 and WRKY40 Function in the Same Genetic Pathway第59-67页
Discussion第67-71页
Conclusion第71-77页
References第77-90页
Abbreviation第90-92页
Acknowledgements第92-93页
在学期间公开发表论文及著作情况第93页

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