| 摘要 | 第7-9页 |
| ABSTRACT | 第9-10页 |
| Purpose and significance of the research | 第14-16页 |
| literature review | 第16-54页 |
| CHAPTER 1 MUSCLE TISSUE | 第16-30页 |
| 1.1 STRUCTURE | 第17-19页 |
| 1.2 SKELETAL STRIATED MUSCLE | 第19-30页 |
| CHAPTER 2 SKELETAL MUSCLE DEVELOPMENT AND MYOFIBRILLOGENESIS | 第30-46页 |
| 2.1 PRENATAL MYOGENESIS | 第30-32页 |
| 2.2 POSTNATAL MUSCLE GROWTH | 第32-33页 |
| 2.3 HORMONALAND REGULATORY PROTEIN CONTROL OF MUSCLE DEVELOPMENT | 第33-34页 |
| 2.4 INVERSE RELATIONSHIP BETWEEN MUSCLE FIBRE NUMBER AND SIZE | 第34-35页 |
| 2.5. FACTORS INFLUENCING MUSCLE FIBRE NUMBER AND SIZE | 第35-43页 |
| 2.5.1 Species | 第35-36页 |
| 2.5.2 Gender | 第36页 |
| 2.5.3 Selection and breed | 第36-39页 |
| 2.5.4 Nutrition | 第39-40页 |
| 2.5.5 Physical activity | 第40-42页 |
| 2.5.6 Influence of major genes | 第42-43页 |
| 2.6 RELATIONSHIP BETWEEN MYOSIN ISOFORMS AND MEAT QUALITY TRAITS | 第43-44页 |
| 2.7 GENETIC IMPROVEMENT OF ANIMAL PERFORMANCE BY MUSCLE FIBRE TRAITS | 第44-46页 |
| CHAPTER 3 MYOTILIN | 第46-54页 |
| 3.1 IDENTIFICATION AND CHARACTERIZATION OF MYOTILIN | 第46-47页 |
| 3.2 STRUCTURE OF MYOTILIN AND HOMOLOGY WITH OTHER INTRACELLULAR IG- FAMILY PROTEINS | 第47页 |
| 3.3 ORGANIZATION AND CHROMOSOMAL LOCALIZATION OF THE MYOTILIN GENE | 第47-48页 |
| 3.4 EXPRESSION OF MYOTILIN IN ADULT TISSUES | 第48-49页 |
| 3.5 EXPRESSION OF MYOTILIN IN FETAL TISSUES | 第49-50页 |
| 3.6 EXPRESSION OF ENDOGENOUS MYOTILIN IN DIFFERENTIATING MUSCLE CELLS | 第50-51页 |
| 3.7 THE ROLE OF MYOTILIN IN Z-DISC FORMATION | 第51-52页 |
| 3.8. ASSOCIATION OF MYOTILIN WITH LGMD1A | 第52-54页 |
| RESEARCH WORK | 第54-85页 |
| CHAPTER 4 BOVINE MYOTILIN GENE (MYOT) POLYMORPHISMS AND THEIR ASSOCIATION STUDY WITH MEAT PRODUCTION TRAITS | 第54-63页 |
| 4.1 MATERIAL AND METHODS | 第54-56页 |
| 4.1.1 Genomic DNA isolation and traits measurement | 第54页 |
| 4.1.2 Primers design and polymerase chain reaction (PCR) | 第54-55页 |
| 4.1.3 Restriction fragment length polymorphism | 第55页 |
| 4.1.4 Single-strand conformation polymorphism (SSCP) analysis | 第55页 |
| 4.1.5 Statistical analysis | 第55-56页 |
| 4.2 RESULTS | 第56-62页 |
| 4.3 DISCUSSION | 第62-63页 |
| CHAPTER 5: BOVINE MYOT GENE CLONING, MRNA EXPRESSION PROFILING AND BIOINFORMATICS STUDY | 第63-70页 |
| 5.1 MATERIAL AND METHODS | 第63-65页 |
| 5.1.1 Bovine MYOT cDNA cloning | 第63页 |
| 5.1.2 RNA extraction and cDNA synthesis | 第63页 |
| 5.1.3 Real time PCR | 第63-64页 |
| 5.1.4 Bioinformatic Study | 第64页 |
| 5.1.5 Statistical analysis | 第64-65页 |
| 5.2 RESULTS | 第65-68页 |
| 5.2.1 mRNA expression analysis | 第65页 |
| 5.2.2 MYOT gene cDNA isolation and MYOT protein comparison | 第65-68页 |
| 5.3 DISCUSSION | 第68-70页 |
| CHAPTER 6: LENTIVIRUS-MEDIATED MYOT GENE INHIBITION REGULATE MYOFIBER FORMATION IN BOVINE MUSCLE CELL | 第70-85页 |
| 6.1 MATERIAL AND METHODS | 第70-76页 |
| 6.1.1 Isolation, initial culturing and maintenance of Bovine MSC | 第70-72页 |
| 6.1.2 Myoblast differentiation | 第72页 |
| 6.1.3 Lentivirus production | 第72页 |
| 6.1.4 Multiplicity of infection (MOI) test | 第72-73页 |
| 6.1.5 Lentiviral construct knockdown efficiency test | 第73页 |
| 6.1.6 Target cell transduction | 第73页 |
| 6.1.7 RNA extraction, Reverse Transcriptase-PCR and Real Time-PCR | 第73-74页 |
| 6.1.8 Protein extraction and western blot | 第74-75页 |
| 6.1.9 Statistical analysis | 第75-76页 |
| 6.2 RESULTS | 第76-82页 |
| 6.2.1 Bovine MSC isolation and initial culturing | 第76页 |
| 6.2.2 Myotube Formation | 第76-77页 |
| 6.2.3 Longitudinal expression profile comparison of myotube markers and MYOT gene | 第77-78页 |
| 6.2.4 Multiplicity of infection (MOI) test | 第78页 |
| 6.2.5 Lentiviral construct knockdown efficiency test | 第78-79页 |
| 6.2.6 Bovine MYOT gene down regulation experiment | 第79-80页 |
| 6.2.7 Myofiber as affected by MYOT gene | 第80-82页 |
| 6.3 Discussion | 第82-85页 |
| Conclusion | 第85-86页 |
| References | 第86-99页 |
| Acknowledgement | 第99-101页 |
| Author Introduction | 第101页 |
