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Effects of Octylphenol on Growth and Development of Rana Chensinensis Tadpoles via Disrupting Thyroid Function

摘要第3-5页
Abstract第5-7页
Chapter 1 Reviews about EDCs Impact on Growth and Development viaDisrupting Thyroid Function第10-22页
    1 Functions of THs and TRs第10-12页
    2 THs control amphibian metamorphosis第12-13页
    3 EDCs impact on reproductive and development第13-16页
    4 EDCs impact on Dio2 and Dio3第16-17页
    5 EDCs impact on THs and TRs第17-21页
    6 The problems and prospect第21-22页
Chapter 2 Effects of Octylphenol on Growth and Development of Tadpole,Rana chensinensis第22-28页
    1. Introduction第22-23页
    2. Materials and methods第23-24页
        2.1 Materials第23页
        2.2 Methods第23页
        2.3 Statistics analysis第23-24页
    3. Results第24-26页
        3.1 Body length of tadpoles第24页
        3.2 Weight of tadpoles第24-25页
        3.3 Developmental times of tadpoles第25-26页
    4. Discussion第26-28页
Chapter 3 Effects of OP on Expression of Deiodinases and ThyroidHormone Receptors Genes of Tadpole, Rana chensinensis第28-48页
    1. Introduction第28-29页
    2. Materials and methods第29-32页
        2.1 Animals第29页
        2.2 Reagents and main instruments第29-30页
        2.3 OP exposure and sample collection第30页
        2.4 Histological analysis第30页
        2.5 RNA extraction and cDNA synthesis第30-31页
        2.6 cDNA amplification第31-32页
        2.7 Quantitative Real-time PCR第32页
    3. Results第32-43页
        3.1 Microscopic structure of thyroid gland第32-35页
        3.2 RNA extraction and Dio2, Dio3 identification第35-37页
        3.3 Expressions of Dio2, Dio3, TRa and TRβ in several tissues of R. chensinensis tadpoles第37-43页
    4. Discussion第43-48页
Summarise第48-50页
Deficiencies in this work第50-52页
References第52-64页
致谢第64-66页
攻读硕士学位期间的研究成果第66页

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