| 摘要 | 第6-8页 |
| ABSTRACT | 第8-9页 |
| ABBREVIATIONS | 第20-21页 |
| CHAPTER1 INTRODUCTION | 第21-34页 |
| 1. The honey bees | 第21-34页 |
| 1.1 The beekeeping industry | 第21-22页 |
| 1.2 Chinese beekeeping industry | 第22-23页 |
| 1.3 Economic importance of the honeybee | 第23-26页 |
| 1.3.1 Honey | 第23-24页 |
| 1.3.2 Beeswax | 第24页 |
| 1.3.3 Royal Jelly | 第24-25页 |
| 1.3.4 Pollen | 第25页 |
| 1.3.5 Other products | 第25-26页 |
| 1.3.6 Pollination | 第26页 |
| 1.4 The honeybee colony and the cast dependent role of worker bees | 第26-29页 |
| 1.4.1 The honeybee queen | 第27-28页 |
| 1.4.2 The honeybee drones | 第28页 |
| 1.4.3 The honeybee workers | 第28-29页 |
| 1.5 Anatomy of the honeybee | 第29-34页 |
| 1.5.1 The Hypopharyngeal Glands (HGs) | 第30-34页 |
| CHAPTER2 QUALITATIVE AND QUANTITATIVE PROTEOME COMPARISON OF HYPOPHARYNGEALGLANDS (HGs) OF ITALIAN HONEYBEES (APIS MELLIFERA) AT DIFFERENT AGES | 第34-51页 |
| 2.1 SUMMARY | 第34-35页 |
| 2.2 INTRODUCTION | 第35页 |
| 2.3 OBJECTIVE | 第35页 |
| 2.4 MATERIALS AND METHOD | 第35-38页 |
| 2.4.1 Reagents | 第35页 |
| 2.4.2 Sampling hypopharyngeal glands of ITBs | 第35-36页 |
| 2.4.3 Protein extraction and digestion | 第36页 |
| 2.4.4 LC?MS/MS analysis | 第36-37页 |
| 2.4.5 Identification and abundance level quantitation of proteins | 第37-38页 |
| 2.4.6 Bioinformatics analysis | 第38页 |
| 2.5 RESULTS | 第38-47页 |
| 2.5.1 Qualitative proteome comparison of HG development in ITBs | 第39-43页 |
| 2.5.2 Quantitative proteome comparison of HG development in ITB | 第43-47页 |
| 2.6 DISCUSSION | 第47-50页 |
| 2.6.1 Proper development and basic functions of the HGs requires shared proteome | 第48页 |
| 2.6.2 Age-dependent development and functions of the HGs requires age-specific proteins | 第48-50页 |
| 2.7 CONCLUSION | 第50-51页 |
| CHAPTER3 QUALITATIVE AND QUANTITATIVE PROTEOME COMPARISON OF HYPOPHARYNGEAL GLANDS (HGs) OF HIGH ROYAL JELLY PRODUCING HONEYBEES (RJBs) (APIS MELLIFERALIGUSTICA) AT DIFFERENT AGES | 第51-67页 |
| 3.1 SUMMARY | 第51-52页 |
| 3.2 INTRODUCTION | 第52页 |
| 3.3 OBJECTIVE | 第52页 |
| 3.4 MATERIALS and METHODS | 第52-53页 |
| 3.4.1 Reagents | 第52-53页 |
| 3.4.2 Sampling hypopharyngeal glands of RJBs | 第53页 |
| 3.5 RESULTS | 第53-62页 |
| 3.5.1 Qualitative proteome comparison of HG development in RJBs | 第54-58页 |
| 3.5.2 Quantitative proteome comparison of HG development in RJBs | 第58-62页 |
| 3.6 Discussion | 第62-66页 |
| 3.6.1 Proper development and basic functions of the HGs requires shared proteome | 第63页 |
| 3.6.2 Age-dependent development and functions of the HGs requires age-specific Proteins | 第63-66页 |
| 3.7 CONCLUSION | 第66-67页 |
| CHAPTER4 PROTEOME COMPARISON OF HYPOPHARYNGEAL GLANDS (HGS) BETWEEN TWO STOCKS OF HONEYBEES REVEALS DEEP INSIGHT INTO MOLECULAR UNDERLINGS OF HGDEVELOPMENT, FUNCTIONALITY AND DIFFERENCE | 第67-98页 |
| 4.1 SUMMARY | 第67-68页 |
| 4.2 INTRODUCTION | 第68-69页 |
| 4.3 OBJECTIVE | 第69-70页 |
| 4.4 MATERIALS AND METHOD | 第70页 |
| 4.4.1 Reagents | 第70页 |
| 4.4.2 Sampling hypopharyngeal lands from ITBs and RJBs | 第70页 |
| 4.5 RESULT | 第70-92页 |
| 4.5.1 Proteome comparison of HG between NEBs from ITBs and RJBs | 第72-78页 |
| 4.5.2 Proteome comparison of HG between NBs from ITBs and RJBs | 第78-84页 |
| 4.5.3 Proteome comparison of HGs between FBs from ITBs and RJBs | 第84-92页 |
| 4.6 DISCUSSION | 第92-97页 |
| 4.6.1 Proper development and basic functions of the NEBs HGs requires shared proteome | 第93页 |
| 4.6.2 The NEBs development from ITBs and RJBs adapt divergent HG proteome | 第93-94页 |
| 4.6.3 Proper development and basic functions of the NBs HGs requires shared Proteome | 第94页 |
| 4.6.4 The NBs development from ITBs and RJBs adapt divergent HG proteome | 第94-95页 |
| 4.6.5 Proper development and basic functions of the FBs HGs requires shared proteome | 第95-96页 |
| 4.6.6 The FBs development from ITBs and RJBs adapt divergent HG proteome | 第96-97页 |
| 4.7 CONCLUSION | 第97-98页 |
| REFERENCES | 第98-109页 |
| ACKNOWLEDGEMENTS | 第109-110页 |
| LIST OF TABLES | 第110-135页 |
| AUTHOR’S BIOGRAPHY | 第135-138页 |
