| ACKNOWLEDGEMENTS | 第1-13页 |
| ABSTRACT | 第13-15页 |
| 中文摘要 | 第15-19页 |
| CHAPTER 1 GENERAL INTRODUCTION AND PROJECT LAYOUT | 第19-22页 |
| CHAPTER 2 REVIEW OF LITERATURE | 第22-50页 |
| ·PLANT DEFENSES | 第22-24页 |
| ·NON-EXPRESSOR OF PATHOGENESIS RELATED GENES 1 (NPR1) | 第24-36页 |
| ·NPR1 in SA mediated SAR | 第25-29页 |
| ·The role of NPR1 in induced systemic resistance (ISR) and JA mediated defense against herbivore (HIR) | 第29-31页 |
| ·Plant defense by NPR1 | 第31-36页 |
| ·ALLENE OXIDE SYNTHASE (AOS) | 第36-50页 |
| ·Copy number of the gene | 第36-37页 |
| ·AOS in the JA signaling pathway | 第37-43页 |
| ·Expression of AOS | 第43-48页 |
| ·Plant defense by AOS | 第48-50页 |
| CHAPTER 3 EXPRESSION PROFILE OF NPR1 IN RICE | 第50-75页 |
| ·INTRODUCTION | 第50-51页 |
| ·MATERIALS AND METHODS | 第51-61页 |
| ·Plant material | 第51-52页 |
| ·Insects | 第52页 |
| ·Plant treatments | 第52-54页 |
| ·Sample processing | 第54-57页 |
| ·Cloning of NPR1 and sequence analysis | 第57-59页 |
| ·Quantitative real-time PCR (qRT-PCR) | 第59-61页 |
| ·RESULTS | 第61-71页 |
| ·Cloning,identification of NPR1 and its sequence analysis | 第61-62页 |
| ·NPR1 protein nucleotide and amino acid sequence | 第62-63页 |
| ·Phylogenetic tree of NPR1 and its homologues | 第63-64页 |
| ·Relative expression levels of NPR1 in different rice tissues | 第64-65页 |
| ·Influence of signaling molecules on the expression levels of NPR1 | 第65-68页 |
| ·Influence of herbivore infestation on expression levels of NPR1 | 第68-71页 |
| ·DISCUSSIONS | 第71-74页 |
| ·Relative expression levels of NPR1 in different rice tissues | 第71-72页 |
| ·Influence of signaling molecules on the expression levels of NPR1 | 第72-73页 |
| ·Influence of herbivore infestation on expression levels of NPR1 | 第73-74页 |
| ·CONCLUSIONS AND FUTURE PROSPECTS | 第74-75页 |
| CHAPTER 4 GENETIC TRANSFORMATION OF NPR1 (NON-EXPRESSOR OF PATHOGENESIS RELATED GENES-1) IN RICE | 第75-94页 |
| ·INTRODUCTION | 第75-76页 |
| ·MATERIALS AND METHODS | 第76-88页 |
| ·Cloning of NPR1 gene fragment into pCAMBIA 1301 vector | 第76-82页 |
| ·Transformation to Agrobacterium | 第82-86页 |
| ·Tissue culture/Callus induction | 第86-87页 |
| ·Bacterium activation and co-cultivation | 第87页 |
| ·Selection of the transformed calli | 第87-88页 |
| ·GUS assay of the resistant calli | 第88页 |
| ·Regeneration of the resistant calli | 第88页 |
| ·RESULTS AND DISCUSSION | 第88-94页 |
| ·Restriction enzyme digestion of DNA fragment | 第88-89页 |
| ·Successful extraction ofpCAMBIA 1301 | 第89页 |
| ·Identification of the recombinant plasmid | 第89-90页 |
| ·Callus induction | 第90页 |
| ·Bacterium and Co-cultivation | 第90-91页 |
| ·Selection and plant regeneration | 第91-93页 |
| ·Transformation efficiency | 第93-94页 |
| CHAPTER 5 CLONING AND GENETIC TRANSFORMATION OF A OS (ALLENE OXIDE SYNTHASE) IN RICE | 第94-109页 |
| ·INTRODUCTION | 第94-95页 |
| ·MATERIALS AND METHODS | 第95-102页 |
| ·Plant material | 第95页 |
| ·Isolation of total RNA | 第95页 |
| ·Reverse Transcription | 第95页 |
| ·Polymerase Chain Reaction | 第95-96页 |
| ·Cloning of AOS gene fragment into pMD19-T vector | 第96-98页 |
| ·Cloning of AOS gene fragment into pCAMBIA 1301 vector | 第98-102页 |
| ·Transformation to Agrobacterium | 第102页 |
| ·RESULTS AND DISCUSSION | 第102-109页 |
| ·Cloning and identification of AOS and its sequence analysis | 第102-103页 |
| ·AOS protein nucleotide and amino acid sequence | 第103-105页 |
| ·Phylogenetic tree of AOS and its homologues in different plant species | 第105-106页 |
| ·Successful extraction of pCAMBIA 1301 | 第106-107页 |
| ·Restriction enzyme digestion of the DNA fragment | 第107页 |
| ·Identification of the recombinant plasmid | 第107-108页 |
| ·Callus induction | 第108页 |
| ·Bacterium and co-cultivation | 第108页 |
| ·GUS Histochemical Assay,election and plant regeneration | 第108页 |
| ·Transformation efficiency | 第108-109页 |
| CHAPTER 6 CONCLUSIONS AND FUTURE PROSPECTS | 第109-112页 |
| ·CONCLUSIONS | 第109-110页 |
| ·RECOMMENDATIONS | 第110-112页 |
| REFERENCES | 第112-147页 |
| APPENDIX | 第147-148页 |
| CHAPTER 7 DIFFERENTIAL ATTRACTION OF PARASITOIDS IN RELATION TO SPECIFICITY OF KAIROMONES FROM HERBIVORE AND ITS BY-PRODUCTS | 第148-187页 |
| CURRICULUM VITAE | 第187-189页 |
