| Acknowledgement | 第1-16页 |
| Summary | 第16-20页 |
| 中文摘要 | 第20-23页 |
| SECTION-A | 第23-53页 |
| CHAPTER 1.GENERAL INTRODUCTION | 第24-35页 |
| ·Cotton-an important cash crop | 第24-25页 |
| ·Cotton plant and its classification | 第24-25页 |
| ·Genetic improvement approaches | 第25页 |
| ·Herbicides menace in plants | 第25-28页 |
| ·Development of herbicide resistance | 第26页 |
| ·Natural resistance | 第26页 |
| ·Introduced resistance | 第26页 |
| ·Crop herbicide selectivity | 第26-27页 |
| ·Genetic engineering for herbicide tolerance | 第27-28页 |
| ·Agrobacterium-a small genetic engineer | 第28-30页 |
| ·T-DNA region of Agrobacterium | 第28-29页 |
| ·Steps in Agrobacterium-mediated gene transformation | 第29-30页 |
| ·Cadmium toxicity syndrome in plants | 第30-32页 |
| ·Aims,strategies and overview of the present study | 第32-35页 |
| ·Aims of the study | 第32页 |
| ·Stepwise approaches/strategies to accomplish the intended objectives | 第32-33页 |
| ·An overview of the whole study | 第33-35页 |
| CHAPTER 2.LITERATURE REVIEW | 第35-53页 |
| ·Genetic engineering in upland cotton via Agrobacterium-mediated technique | 第35-38页 |
| ·Genetic engineering for herbicide tolerance in cotton | 第38-42页 |
| ·Glyphosate-tolerant cotton | 第39-40页 |
| ·Bromoxynil-resistant cotton | 第40-41页 |
| ·Gluphosinate-resistant cotton | 第41-42页 |
| ·Imidazolinone-resistant cotton | 第42页 |
| ·Cadmium toxicity syndrome in plants | 第42-53页 |
| ·Cellular mechanisms in response to cadmium stress | 第43-47页 |
| ·Phytochelatins | 第44-45页 |
| ·Vacuolar compartmentalization | 第45-46页 |
| ·Metallothioneins | 第46-47页 |
| ·Stress Proteins | 第47页 |
| ·Cadmium uptake and translocation | 第47-49页 |
| ·Generation and detoxification of ROS in plants | 第49-53页 |
| SECTION-B | 第53-151页 |
| CHAPTER 3.Studies on genetic transformation of the Bar gene and its inheritance and segregation in the resultant transgenic cotton germplasm | 第54-72页 |
| Abstract | 第54页 |
| ·Introduction | 第54-56页 |
| ·Materials and methods | 第56-62页 |
| ·Plant materials | 第56-57页 |
| ·Agrobacterium strain and plant species | 第57页 |
| ·Transformation methods | 第57-59页 |
| ·Pollen Tube Pathway via Ovarian Injection method (Method-Ⅰ) | 第57-58页 |
| ·Shoot Apex Culture method (Method-Ⅱ) | 第58页 |
| ·Preparation of shoot apex explants | 第58页 |
| ·Agrobacterium co-cultivation | 第58-59页 |
| ·Selection of transgenic plants | 第59页 |
| ·Putative transgenic plants | 第59页 |
| ·Selection and screening of putative transgenic seedlings | 第59-60页 |
| ·Polymerase chain reaction (PCR) analysis | 第60页 |
| ·Southern blot hybridization analysis | 第60-61页 |
| ·Progeny tests | 第61页 |
| ·Identification of herbicide resistance | 第61页 |
| ·Inheritance of the herbicide resistant trait in transgenic cotton germplasm (BR001) | 第61-62页 |
| ·Agronomic characters and fiber quality of the resultant transgenic germplasm | 第62页 |
| ·Data analysis | 第62页 |
| ·Results and analysis | 第62-69页 |
| ·Kanamycin test for the presence of nptⅡ gene | 第62-63页 |
| ·Bioassay test for basta(?) and the comparative analysis of the two transformation methods | 第63-65页 |
| ·3 PCR analysis | 第65-66页 |
| ·Analysis of T1 plants | 第66页 |
| ·Inheritance and segregation of exogenous Bar gene in transgenic cotton germplasm (BR001) | 第66-67页 |
| ·Identification of herbicide resistance | 第67-68页 |
| ·Agronomic characters and fiber quality of BR001 | 第68-69页 |
| ·Discussion | 第69-70页 |
| ·Conclusions | 第70-72页 |
| CHAPTER 4.Agrobacterium-mediated genetic transformation of Cyp81A6 gene in upland cotton using embryogenic callus as explant | 第72-84页 |
| Abstract | 第72页 |
| ·Introduction | 第72-74页 |
| ·Materials and methods | 第74-79页 |
| ·Plant materials and embryogenic callus induction | 第74-76页 |
| ·Transformation and plant regeneration process | 第76-77页 |
| ·Inoculation,co-culture and selection processes | 第76-77页 |
| ·Agrobacterium tumefaciens strain and plasmid vector | 第77-78页 |
| ·Kanamycin and bentazon assays | 第78-79页 |
| ·PCR analysis for putative transformants | 第79页 |
| ·Results and analysis | 第79-82页 |
| ·Embryogenic calli growth and proliferation on selective medium (MSB3) | 第79-80页 |
| ·Confirmation of transformation event by kanamycin and bentazon assay | 第80-81页 |
| ·Molecular analysis of the putative transgenic embryogenic calli | 第81页 |
| ·Somatic embryos production,their germination,maturation and conversion to plantlets | 第81-82页 |
| ·Discussion | 第82-83页 |
| ·Conclusions | 第83-84页 |
| CHAPTER 5.Cadmium-induced functional and ultrastructural alterations in roots of herbicide resistant transgenic cotton germplasm (BR001) | 第84-108页 |
| Abstract | 第84-85页 |
| ·Introduction | 第85-86页 |
| ·Materials and methods | 第86-89页 |
| ·Seed surface sterilization and treatment process | 第86-87页 |
| ·Seed germination assay and measurement of hypocotyl and radical lengths | 第87页 |
| ·Plant growth parameters and tolerance index | 第87-88页 |
| ·Determination of Cd content | 第88页 |
| ·Transmission electron microscopy | 第88-89页 |
| ·Statistical analysis | 第89页 |
| ·Results | 第89-102页 |
| ·Germination assay and radical and hypocotyl lengths | 第89-91页 |
| ·Biomass production,tolerance indices and root tip percentage | 第91-93页 |
| ·Root morphological traits | 第93-94页 |
| ·Bioaccumulation of cadmium in roots and shoots of plants | 第94-95页 |
| ·Ultrastructural observations and features | 第95-102页 |
| ·Discussion | 第102-107页 |
| ·Effect of Cd treatments on plant's qualitative and quantitative parameters | 第102-104页 |
| ·Effect on root morphological parameters | 第104页 |
| ·Effect on Cd accumulation in roots and shoots | 第104-105页 |
| ·Effect on root ultrastructure | 第105-107页 |
| ·Conclusions | 第107-108页 |
| CHAPTER 6.Cadmium-induced ultramorphological and physiological changes in leaves of herbicide resistant transgenic cotton germplasm (BR001) | 第108-132页 |
| Abstract | 第108-109页 |
| ·Introduction | 第109-110页 |
| ·Materials and Methods | 第110-112页 |
| ·Seed surface sterilization and treatment process | 第110-111页 |
| ·Plant growth parameters and Water Absorption Capacity (WAC) | 第111页 |
| ·Transmission electron microscopy | 第111页 |
| ·Statistical analysis | 第111-112页 |
| ·Results | 第112-126页 |
| ·Plant growth responses | 第112页 |
| ·Biomass production | 第112-116页 |
| ·Water Absorption Capacity (WAC) | 第116-117页 |
| ·Correlation among qualitative and quantitative traits of the seedlings | 第117-120页 |
| ·Ultrastructural changes under Cd treatments | 第120-126页 |
| ·Discussion | 第126-130页 |
| ·Plant growth responses | 第126-127页 |
| ·Biomass production | 第127页 |
| ·Water Absorption Capacity (WAC) | 第127-128页 |
| ·Correlation among qualitative and quantitative traits of the seedlings | 第128页 |
| ·Ultrastructural changes under Cd stress | 第128-130页 |
| ·Conclusions | 第130-132页 |
| CHAPTER 7.Cadmium-induced oxidative metabolism in herbicide resistant transgenic cotton germplasm (BR001) | 第132-147页 |
| Abstract | 第132页 |
| ·Introduction | 第132-133页 |
| ·Materials and methods | 第133-135页 |
| ·Plant materials and growth conditions | 第133-134页 |
| ·Determination of antioxidant enzymes activities and Lipid peroxidation | 第134-135页 |
| ·Statistical analysis | 第135页 |
| ·Results | 第135-143页 |
| ·Effect of Cd on APOX activity | 第135-136页 |
| ·Effect of Cd on POX activity | 第136-138页 |
| ·Effect of Cd on SOD activity | 第138页 |
| ·Effect of Cd on CAT activity | 第138-141页 |
| ·Effect of Cd on MDA activity | 第141-143页 |
| ·Discussion | 第143-145页 |
| ·APOX activity under Cd stress | 第143-144页 |
| ·POX activity under Cd stress | 第144页 |
| ·SOD activity under Cd stress | 第144-145页 |
| ·CAT activity under Cd stress | 第145页 |
| ·MDA activity under Cd stress | 第145页 |
| ·Conclusions | 第145-147页 |
| CHAPTER 8.Major findings and future recommendations | 第147-151页 |
| ·Major Findings | 第147-149页 |
| ·Future Recommendations | 第149-151页 |
| References | 第151-177页 |
| Curriculum Vitae | 第177-180页 |
