| Abstract | 第1-9页 |
| 摘要 | 第9-12页 |
| Abbreviations | 第12-13页 |
| Part I Introduction | 第13-15页 |
| Part II Preparation of retrovirus expressing transcription factors | 第15-27页 |
| ·Materials, reagent and equipment | 第15-17页 |
| ·Plasmids and cells | 第15页 |
| ·Reagents and chemicals | 第15-16页 |
| ·Equipments | 第16页 |
| ·Reagent preparation | 第16-17页 |
| ·Methods | 第17-22页 |
| ·Plasmid preparation | 第17-19页 |
| ·package cell line culture | 第19页 |
| ·retrovirus production | 第19-20页 |
| ·Functional test of retrovirus | 第20-22页 |
| ·Results and analysis | 第22-25页 |
| ·Plasmid preparation | 第22页 |
| ·Retrovirus production | 第22页 |
| ·Functional tests of retrovirus | 第22-25页 |
| ·Discussion | 第25-26页 |
| ·Summary | 第26-27页 |
| Part III Reprogramming of rat embryonic fibroblasts by 4 factors | 第27-45页 |
| ·Materials, reagent and equipment | 第27-29页 |
| ·Animal, cell, plasmid | 第27页 |
| ·Reagent and chemicals | 第27-28页 |
| ·Equipments | 第28页 |
| ·Reagent preparation | 第28-29页 |
| ·Methods | 第29-37页 |
| ·Preparation and culture of rat embryonic fibroblasts | 第29-30页 |
| ·Generation of 4 factor reprogrammed rat embryonic fibroblasts | 第30-31页 |
| ·Establishment and maintenance of colony culture | 第31-32页 |
| ·Characterization of reprogrammed cells | 第32-37页 |
| ·Results and analysis | 第37-42页 |
| ·Rat embryonic fibroblast culture | 第37-38页 |
| ·Reprogramming of rat embryonic fibroblasts with 4 factors | 第38-40页 |
| ·Differentiation capacity of 4 factors reprogrammed cells | 第40-42页 |
| ·Discussion | 第42-44页 |
| ·Summary | 第44-45页 |
| Part IV Reprogramming of rat embryonic fibroblasts by 3 factors | 第45-63页 |
| ·Materials, reagent and equipment | 第45-48页 |
| ·Animal, cell, plasmid | 第45页 |
| ·Reagent and chemicals | 第45-46页 |
| ·Equipments | 第46-47页 |
| ·Reagent preparation | 第47-48页 |
| ·Methods | 第48-56页 |
| ·Preparation and culture of rat embryonic fibroblasts | 第48-49页 |
| ·Generation of 3 factor reprogrammed rat embryonic fibroblasts | 第49页 |
| ·Establishment and maintenance of colony culture | 第49-51页 |
| ·Characterization of reprogrammed cells | 第51-52页 |
| ·Differentiation of 3 factor reprogrammed cells | 第52-56页 |
| ·Results and analysis | 第56-61页 |
| ·Establishment of single colony culture | 第56-57页 |
| ·Characterization of 3 factor reprogrammed cells | 第57-59页 |
| ·Differentiation capacity of 3f reprogrammed cells | 第59-61页 |
| ·Discussion | 第61-62页 |
| ·Summary | 第62-63页 |
| Conclusions | 第63-64页 |
| Reference | 第64-68页 |
| Published paper | 第68-69页 |
| ACKNOWLEDGEMENT | 第69页 |
