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外源24-表油菜素内酯和乙酰水杨酸处理及热驯化诱导番茄耐热性研究

ABSTRACT第5-7页
摘要第8-16页
Chapter 1 Introduction and Review of Literature第16-41页
    1.1 Effect of high temperature on plant growth第16-19页
        1.1.1 Thermal tolerance in plants第17页
        1.1.2 Seed germination response under heat stress第17页
        1.1.3 Morphological symptoms caused by heat stress第17-18页
        1.1.4 Heat stress and photosynthetic performance第18-19页
    1.2 Effect of high temperature on reproductive growth of plant第19-22页
        1.2.1 Effect of heat stress on flowering第19-20页
        1.2.2 Effect of heat stress on pollen performance第20-21页
        1.2.3 Fruit set under heat stress conditions第21页
        1.2.4 Effect of heat stress on yield第21-22页
    1.3 Effect of heat stress on tomato plants第22-26页
        1.3.1 Heat stress induced flower shed in tomato第25页
        1.3.2 Heat stress induced anther cone opening and style elongation in tomato flowers第25-26页
    1.4 Assessment of heat stress effects第26-27页
    1.5 Strategies to manage heat stress第27页
    1.6 Heat acclimation第27-30页
        1.6.1 Heat shock proteins第28-29页
        1.6.2 Role of heat acclimation in survival and morphological characteristics第29页
        1.6.3 Heat acclimation and oxidative stress第29-30页
        1.6.4 Role of heat acclimation for membrane stability and reproductive growth第30页
    1.7 Brassinosteroids第30-36页
        1.7.1 Role of BRs in stress tolerance第30-31页
        1.7.2 BR induced heat shock proteins第31页
        1.7.3 Role of BRs to protect photosynthetic machinery under heat stress第31-32页
        1.7.4 Role of BRs in biomass production and survival第32-33页
        1.7.5 Effect of BRs on yield in heat stressed tomato plants第33页
        1.7.6 Role of BRs in antioxidant enzymes activity第33-34页
        1.7.7 Effect of BRs on dry mass in heat stressed plants第34页
        1.7.8 Effect of BRs on necrotic leaves of plants under heat stress第34页
        1.7.9 Role of BRs in protection of protein synthesis under heat stress第34页
        1.7.10 Role of BRs on root growth第34-35页
        1.7.11 Role of BRs on chlorophyll contents in heat stressed plants第35页
        1.7.12 Effect of BRs on plant morphological features during heat stress第35页
        1.7.13 Role of BRs in heat stress amelioration in tomato第35-36页
    1.8 Acetyl salicylic acid for induction of heat tolerance in plants第36-39页
        1.8.1 Role of SA/ASA in antioxidant activity第37-38页
        1.8.2 Role of SA in photosynthesis in heat stressed plants第38页
        1.8.3 Effect of SA on cell integrity in stressed plants第38页
        1.8.4 Growth responses of ASA/SA in stressed plants第38-39页
    1.9 Need of the present study第39-40页
    1.10 Objectives第40-41页
Chapter 2 Effect of Exogenous Acetyl Salicylic Acid and 24-Epibrassinolide on thePhotosynthesis, Chlorophyll contents and Membrane Thermostability of TomatoSeedlings Grown under Heat Stress in Growth Chamber第41-55页
    2.1 Introduction第41-43页
    2.2 Materials and Methods第43-46页
        2.2.1 Growing seedlings第43页
        2.2.2 Chemical treatments第43-44页
        2.2.3 Heat treatment第44页
        2.2.4 Photosynthesis第44-45页
        2.2.5 Chlorophyll contents第45页
        2.2.6 Cell membrane thermostability第45-46页
        2.2.7 Experimental design and data analysis第46页
    2.3 Results第46-52页
        2.3.1 Plant survival第46-47页
        2.3.2 Effect on membrane thermostability第47-48页
        2.3.3 Effect on vegetative growth第48页
        2.3.4 Effect on reproductive growth第48-49页
        2.3.5 Effect on chlorophyll contents第49-50页
        2.3.6 Effect on photosynthesis第50-52页
    2.4 Discussion第52-54页
    2.5 Summary第54-55页
Chapter 3 Effect of Acetyl Salicylic Acid and 24-Epibrassinolide on Root Activity andRoot Morphological Features in Tomato Plants Grown under Heat Stress in GrowthChamber第55-67页
    3.1 Introduction第55-58页
    3.2 Materials and Methods第58-60页
        3.2.1 Growing seedlings第58页
        3.2.2 Chemical treatments第58-59页
        3.2.3 Heat treatment第59页
        3.2.4 Root architecture第59页
        3.2.5 Root activity第59-60页
        3.2.6 Experimental design and data analysis第60页
    3.3 Results第60-63页
        3.3.1 Root architecture第60-62页
            3.3.1.1 Root length第60-61页
            3.3.1.2 Surface area第61页
            3.3.1.3 Root volume and diameter第61页
            3.3.1.4 No. of root connections and nodes第61-62页
        3.3.2 Root activity第62-63页
    3.4 Discussion第63-65页
    3.5 Summary第65-67页
Chapter 4 Effect of 24-Epibrassinolide and Acetyl Salicylic Acid on Tomato PlantPerformance Grown under Acute Heat Stress Conditions in Plastic Tunnel第67-101页
    4.1 Introduction第67-68页
    4.2 Materials and Methods第68-73页
        4.2.1 Site description and experimental design第68-69页
        4.2.2 Chemical treatments第69页
        4.2.3 Measurement of heat stress第69-70页
        4.2.4 Photosynthesis第70页
        4.2.5 Chlorophyll contents第70页
        4.2.6 Cell membrane thermostability第70-71页
        4.2.7 Plant enzymes第71页
        4.2.8 Plant growth第71页
        4.2.9 Fruit quality第71-73页
        4.2.10 Nutrient contents in stem and roots第73页
        4.2.11 Statistical analysis第73页
    4.3 Results第73-95页
        4.3.1 Plant growth第73-80页
            4.3.1.1 Effect of EBL and ASA on vegetative growth under acute heat stress第74-76页
                4.3.1.1.1 Plant height第74页
                4.3.1.1.2 Stem thickness第74页
                4.3.1.1.3 Leaf count第74-75页
                4.3.1.1.4 Leaf area第75-76页
                4.3.1.1.5 Stem and root dry mass accumulation第76页
                4.3.1.1.6 Biomass produced第76页
            4.3.1.2 Effect of EBL and ASA on reproductive growth under acute heat stress第76-80页
                4.3.1.2.1 Flower count per plant第77页
                4.3.1.2.2 Flower drop per plant第77-78页
                4.3.1.2.3 Fruit count per plant第78页
                4.3.1.2.4 Seed count per fruit第78-79页
                4.3.1.2.5 Yield per plant第79-80页
        4.3.2 Membrane thermostability第80-81页
        4.3.3 Effect of chemical treatment on leaf-tip Sun burn第81页
        4.3.4 Fruit quality第81-86页
            4.3.4.1 Fruit pH and TSS (°Brix)第81-82页
            4.3.4.2 Fruit soluble sugar第82-83页
            4.3.4.3 Vitamin C contents第83-84页
            4.3.4.4 Protein contents第84-86页
            4.3.4.5 Fruit dry mass (%)第86页
        4.3.5 Leaf chlorophyll contents第86-89页
        4.3.6 Photosynthetic performance第89页
        4.3.7 Antioxidant enzymes第89-93页
        4.3.8 Effect of 24-EBL and ASA on NPK contents in stem and roots第93-95页
    4.4 Discussion第95-98页
    4.5 Estimation of economic feasibility of the 24-EBL and ASA application (per acre basis)第98-99页
        4.5.1 Economic feasibility of 24-EBL第98页
        4.5.2 Economic feasibility of Acetyl-SA第98-99页
    4.6 Summary第99-101页
Chapter 5 Performance Evaluation of Heat Acclimated Tomatoes in Controlled andField Conditions第101-119页
    5.1 Introduction第101-102页
    5.2 Materials and Methods第102-106页
        5.2.1 Effect of heat acclimation on thermotolerance of tomato seedlings in controlled conditions第102-104页
            5.2.1.1 Growing seedlings第102-103页
            5.2.1.2 Heat acclimation第103页
            5.2.1.3 Heat stress treatment第103页
            5.2.1.4 Survival第103页
            5.2.1.5 Cell membrane thermostability第103-104页
        5.2.2 Performance evaluation of heat acclimated tomato plants under moderate (open field) and chronic heat stress (plastic tunnel)第104-106页
            5.2.2.1 Photosynthesis第104-105页
            5.2.2.2 Chlorophyll contents第105页
            5.2.2.3 Vegetative and reproductive plant growth第105页
            5.2.2.4 Metrological data第105-106页
        5.2.3 Experimental design and data analysis第106页
    5.3 Results and Discussion第106-117页
        5.3.1 Survival against lethal heat shock第106-107页
        5.3.2 Cell membrane thermostability in growth chamber seedlings第107页
        5.3.3 Survival under field conditions第107-108页
        5.3.4 Membrane thermostability of field plants第108-109页
        5.3.5 Chlorophyll contents第109-111页
        5.3.6 Photosynthetic performance第111-113页
        5.3.7 Vegetative growth under chronic heat stress conditions (inside plastic tunnel)第113-114页
        5.3.8 Reproductive growth at chronic heat stress conditions (inside plastic tunnel)第114-116页
            5.3.8.1 Flower第114-115页
            5.3.8.2 Fruit第115-116页
        5.3.9 Vegetative growth at moderate heat stress conditions第116页
        5.3.10 Reproductive growth at moderate heat stress conditions第116-117页
            5.3.10.1 Flower第116-117页
            5.3.10.2 Fruit第117页
    5.4 Summary第117-119页
Chapter 6 Effect of 24-Epibrassinolide on Pollen Germination第119-125页
    6.1 Introduction第119-121页
    6.2 Materials and methods第121-122页
        6.2.1 Collection of flowers第121页
        6.2.2 Pollen growth medium (PGM) and preparation of treatments第121-122页
        6.2.3 Yielding pollen, inocculation and incubation第122页
        6.2.4 Data collection and statistical analysis第122页
    6.3 Results and Discussion第122-124页
    6.4 Summary第124-125页
REFERENCES第125-146页
ACKNOWLEDGEMENT第146-147页
ABOUT THE AUTHOR第147页

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