Abstract (in English) | 第1-13页 |
Abstract (in Chinese) | 第13-15页 |
Part one The studies of piezoelectric biosensors and detection of staphylococcal enterotoxins | 第15-69页 |
Chapter one The progresses in piezoelectric biosensors and detection of staphylococcal enterotoxins | 第15-44页 |
Section one The progresses in piezoelectric biosensors | 第15-32页 |
1 The principle and constitutes of piezoelectric biosensor | 第16-20页 |
·The principle and constitutes of piezoelectric sensor with bulk acoustic waves | 第17-18页 |
·The principle and constitutes of piezoelectric sensor with surface acoustic waves | 第18-20页 |
2 The classify of piezoelectric biosensor | 第20页 |
3 Surface modification technique in piezoelectric biosensor | 第20-21页 |
4 The progresses in piezoelectric biosensor applications | 第21-26页 |
·Immunoassay | 第21-22页 |
·Detection of the gene | 第22-24页 |
·Detection of microorganism | 第24页 |
·Other matters testing | 第24-25页 |
·Piezoelectric biosensors coupled with other techniques | 第25-26页 |
5 The trend of development | 第26页 |
References | 第26-32页 |
Section two The progresses in detection of Staphylococcus aureus and staphylococcal enterotoxins | 第32-44页 |
1 The research in classify and typology of Staphylococcus aureus | 第32-34页 |
·Classify of Staphylococcus aureus | 第32-33页 |
·Typology of Staphylococcus aureus | 第33-34页 |
2 Toxicological study of Staphylococcus aureus | 第34-35页 |
3 The study for determination of Staphylococcus aureus and their toxins | 第35-39页 |
·Measuring with biological methods | 第35页 |
·Methods of immunoserology | 第35-36页 |
·PCR technique | 第36-37页 |
·Nucleic acid hybridization technique | 第37-38页 |
·Biosensor technique | 第38页 |
·Superantigen technique | 第38-39页 |
References | 第39-44页 |
Chapter two Detecting the gene of staphylococcal enterotoxin B using an surface acoustic wave biosensor | 第44-58页 |
Abstract | 第44页 |
1 Introduction | 第44-46页 |
2 Experimental | 第46-48页 |
·Apparatus and materials | 第46-47页 |
·Procedures | 第47-48页 |
3 Results and discussion | 第48-54页 |
·The stability of SAW biosensor | 第48-49页 |
·Pretreatment of biosensor surface and DNA probe immobilization | 第49-51页 |
·Hybridization and real sample detection | 第51-53页 |
·Specificity of the detection | 第53页 |
·Regeneration of the gene biosensor | 第53-54页 |
·Storage condition of the biosensor | 第54页 |
4 Conclusion | 第54页 |
Acknowledgment | 第54-55页 |
References | 第55-58页 |
Chapter three Detection of the gene of staphylococcal enterotoxin B employing a quartz crystal microbalance biosensor | 第58-69页 |
Abstract | 第58页 |
1 Introduction | 第58-59页 |
2 Experimental | 第59-62页 |
·Apparatus and materials | 第60-61页 |
·Procedures | 第61-62页 |
3 Results and discussion | 第62-65页 |
·Cleaning and renewing of the crystal surface | 第62页 |
·Measure of crystal oscillators stability | 第62页 |
·DNA probe immobilization | 第62-64页 |
·Hybridization and sample detection | 第64-65页 |
·Regeneration of the gene biosensor | 第65页 |
4 Conclusion | 第65-66页 |
References | 第66-69页 |
Part two Chemiluminescence behavior based on oxidation reaction of rhodamine B with cerium(Ⅳ) in sulfuric acid medium | 第69-86页 |
Abstract | 第69页 |
1 Introduction | 第69-70页 |
2 Experimental | 第70-72页 |
·Reagents and apparatus | 第70页 |
·Procedure | 第70-72页 |
3 Results and discussion | 第72-81页 |
·The selection of the oxidants | 第72页 |
·Optimization of experimental conditions | 第72-74页 |
·Kinetic curve | 第74-75页 |
·Luminescence spectrograms | 第75-76页 |
·The UV/Vis and IR spectra | 第76-78页 |
·Mechanism of CL reaction | 第78-81页 |
4 Conclusion | 第81-82页 |
References | 第82-86页 |
Acknowledgment | 第86-87页 |
Publications | 第87页 |