| Abstract | 第9-11页 |
| CHAPTER 1 LITERATURE REVIEW | 第12-30页 |
| 1.1 INTRODUCTION | 第12-13页 |
| 1.2 SHRIMP ENDOCRINOLOGY A REVIEW | 第13-19页 |
| 1.2.1 Ecdysteroids | 第13-14页 |
| 1.2.2 Molt-inhibiting hormone (MIH) | 第14-15页 |
| 1.2.3 Crustacean hyperglycemic hormone (CHH) | 第15-16页 |
| 1.2.4 Vitellogenesis-inhibiting hormone (VIH) | 第16-17页 |
| 1.2.5 Methyl farnesoate | 第17页 |
| 1.2.6 Chromatophorotropins | 第17-18页 |
| 1.2.7 Other factors | 第18-19页 |
| 1.3 CRUSTACEANS MOLTING | 第19-21页 |
| 1.3.1 Overview | 第19-20页 |
| 1.3.2 Crustacean cuticle structure | 第20页 |
| 1.3.3 Mechanism of molting (ecdysis) | 第20-21页 |
| 1.4 MOLTING HORMONES | 第21-24页 |
| 1.4.1 Ecdysteroids | 第21-24页 |
| 1.4.1.1 Ecdysteroid measurement | 第22-24页 |
| (1) Radioimmunoassay (RIA) | 第22-23页 |
| (2) High-performance liquid chromatography (HPLC) | 第23页 |
| (3) Inositol triphosphate (IP3) and diacylglycerol (DAG) measurements | 第23页 |
| (4) Cell permeabilization and added IP3 | 第23-24页 |
| 1.5 MOLT CYCLE | 第24-25页 |
| 1.6 MOLECULAR CONTROL OF MOLTING IN CRUSTACEANS | 第25页 |
| 1.7 ECDYSONE RECEPTOR | 第25-26页 |
| 1.8 ECDYSONE INDUCIBLE GENES | 第26-27页 |
| 1.9 SIGNIFICANT PROGRESS AND ADVANCES IN RESEARCH ON MOLT RELATED GENES IN CRUSTACEAN MOLTING | 第27-28页 |
| 1.10 SUMMARY | 第28-29页 |
| 1.11 AIMS OF THE THESIS | 第29-30页 |
| CHAPTER 2 MICROSCOPIC EXAMINATION OF MOLTING STAGES IN CHINESE SHRIMP FENNEROPENAEUS CHINENSIS | 第30-40页 |
| 2.1 INTRODUCTION | 第30-32页 |
| 2.2 MOLT STAGING IN CHINESE SHRIMP FENNEROPENAEUS CHINENSIS | 第32-38页 |
| 2.3 DISCUSSION | 第38-40页 |
| CHAPTER 3 ECDYSONE INDUCIBLE GENE E75: MOLECULAR CLONING, EXPRESSION AND EFFECT OF dsRNA SILENCING ON THE MOLTING OF CHINESE SHRIMP FENNERPENAEUS CHINENSIS | 第40-73页 |
| 3.1 INTRODUCTION | 第40-43页 |
| 3.2 METHODS | 第43-58页 |
| 3.2.1 Preparation of total RNA and cDNAsynthesis | 第43-46页 |
| 3.2.2 Initial amplification of partial fragment of FcE75 | 第46-49页 |
| 3.2.3. SMART-RACE of FcE75 | 第49-51页 |
| 3.2.3.1 5 ′ RACE | 第50页 |
| 3.2.3.2 3′ RACE | 第50-51页 |
| 3.2.4 SEQUENCE ANALYSIS | 第51页 |
| 3.2.5 MULTIPLE ALIGNMENT & PHYLOGENETIC TREE CONSTRUCTION | 第51-52页 |
| 3.2.6 QUANTIFICATION OF FcE75 TRANSCRIPTS BY REAL TIME PCR | 第52-53页 |
| 3.2.7 dsRNA PREPARATION | 第53-56页 |
| 3.2.8 SILENCING OF FcE75 GENE BY dsRNA | 第56-58页 |
| 3.2.9 OBSERVATION OF MOLTING AND MORTALITY RATE OF EXPERIMENTAL SHRIMP | 第58页 |
| 3.3 RESULTS | 第58-69页 |
| 3.3.1 TOTAL RNA EXTRACTION | 第58-59页 |
| 3.3.2 AMPLIFICATION OF cDNA AND SEQUENCE ANALYSIS OF FcE75 | 第59-63页 |
| 3.3.3 TISSUE SPECIFIC EXPESSION OF FcE75 | 第63页 |
| 3.3.4 CHANGES IN mRNA EXPRESSION OF FcE75 THROUGH MOLTING STAGES | 第63-65页 |
| 3.3.5 dsRNA PREPARATION AND INJECTION INTO ANIMALS | 第65页 |
| 3.3.6 DOSE-DEPENDENT SILENCING RESPONSE OF FcE75 dsRNA | 第65-66页 |
| 3.3.7 EFFECT OF dsRNA INJECTION ON MOLTING | 第66-68页 |
| 3.3.8 EFFECT OF E75 dsRNA ON MORTALITY OF SHRIMP | 第68-69页 |
| 3.4 DISCUSSION | 第69-73页 |
| CHAPTER 4 ECDYSONE INDUCIBLE GENE RXR: GENOMIC ORGANIZATION, TWO mRNA ISOFORMS, EXPRESSION ANALYSIS AND dsRNA SILENCING IN CHINESE SHRIMP FENNERPENAEUS CHINENSIS | 第73-92页 |
| 4.1 INTRODUCTION | 第73-75页 |
| 4.2 METHODS | 第75-81页 |
| 4.2.1 CLONING AND SEQUENCING OF FULL LENGTH FcRXR CDNA | 第75-77页 |
| 4.2.2 SEQUENCE ANALYSIS AND PHYLOGENETIC ANALYSIS OF FcRXR | 第77-78页 |
| 4.2.3 GENOMIC ORGANIZATION OF FcRXR | 第78-79页 |
| 4.2.4 QUANTIFICATION OF FcRXR TRANSCRIPTS BY REAL TIME PCR | 第79-80页 |
| 4.2.5 dsRNA PREPARATION AND INJECTION INTO ANIMALS | 第80页 |
| 4.2.6 SILENCING OF FcRXR GENE BY dsRNA | 第80-81页 |
| 4.3. RESULTS | 第81-90页 |
| 4.3.1 AMPLIFICATION OF cDNA AND SEQUENCE ANALYSIS OF FcRXR | 第81-85页 |
| 4.3.2 GENOMIC ORGANIZATION OF FcRXR | 第85-88页 |
| 4.3.3 TISSUE SPECIFIC EXPRESSION OF FcRXR | 第88-89页 |
| 4.3.4 CHANGES IN FcRXR EXPRESSION THROUGH MOLTING STAGES | 第89页 |
| 4.3.5 PREPARATION OF dsRXR | 第89-90页 |
| 4.3.6 SILENCING RESPONSE OF dsRXR | 第90页 |
| 4.4 DISCUSSION | 第90-92页 |
| CHAPTER 5 CONSEQUENCE OF dsE75 & dsRXR SILENCING - potential (POSSIBLE) LINK | 第92-99页 |
| 5.1 INTRODUCTION | 第92-94页 |
| 5.2 METHODS | 第94-95页 |
| 5.2.1 QUANTIFICATION OF FcChi & FcChi-1 TRANSCRIPT BY REAL TIME PCR | 第94-95页 |
| 5.2.2 DETECTION OF FcRXR, FcChi & FcChi-1 TRANSCRIPTS IN dsE75 SILENCED SHRIMP | 第95页 |
| 5.2.3 DETECTION OF FcE75, FcChi & FcChi-1 TRANSCRIPTS IN dsRXR SILENCED SHRIMP | 第95页 |
| 5.3 RESULTS | 第95-98页 |
| 5.3.1 EXPRESSION OF FcChi mRNAS AT DIFFERENT MOLT STAGES | 第95-96页 |
| 5.3.2 EXPRESSION OF FcChi-1 mRNA AT DIFFERENT MOLT STAGES | 第96页 |
| 5.3.3 CONSEQUENCE OF dsE75 SILENCING ON RXR, FcChi & FcChi-1 | 第96-97页 |
| 5.3.4 CONSEQUENCE OF dsRXR SILENCING ON E75, FcChi & FcChi-1 | 第97-98页 |
| 5.4 DISCUSSION | 第98-99页 |
| CHAPTER 6 CONCLUSION AND FUTURE DIRECTIONS | 第99-103页 |
| 6.1 SUMMARY & CONCLUSION | 第99-102页 |
| 6.2 FUTURE DIRECTIONS | 第102-103页 |
| REFERENCES | 第103-126页 |
| Publications | 第126-127页 |
| ACKNOWLEDGEMENTS | 第127-129页 |
