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Molecular Cloning of the Rhyzopertha Dominica and Antennal Sensilla of Sitophilus Granarius,Tribolium Castaneum and Their Host Range

摘要第7-9页
Abstract第9-11页
CHAPTER Ⅰ. LITERATURE REVIEW第13-30页
    1.1. Introduction第13-14页
    1.2. The three stored-product pests第14-17页
        1.2.1 Rhyzopertha dominica第15页
        1.2.2 Tribolium castaneum第15-16页
        1.2.3 Sitophilus granarius第16-17页
    1.3. Olfaction in insects第17页
    1.4. The olfactory system第17-19页
    1.5. THE INSECT CHEMOSENSORY SYSTEM第19-21页
        1.5.1. Host plant selection in insects第19-21页
    1.6. Chemical detection and integration第21-29页
        1.6.1 Chemical detection at the antennal level第21-29页
    1.7. Objectives of this study第29-30页
Chapter Ⅱ. The influence of grain cracked on reproduction of sitophilus granarious(Coleoptera: curculionidae) on different types of host grains第30-40页
    2.1. Introduction第30-31页
    2.2. Material and Methods:第31-33页
        2.2.1 Insect第31-32页
        2.2.2 Sitophilus granarius rearing on Different host grains第32页
        2.2.3 Sitophilus granarius adult weight第32-33页
        2.2.4 Data analysis第33页
    2.3. Result & Discussion第33-39页
        2.3.1 Sitophilus granarius demonstration on different host grains第33-36页
        2.3.2. Sitophilus granarius adult weight第36-39页
    2.4. Conclusion第39-40页
Chapter Ⅲ. Effect of broken grains on reproduction of Rhyzopertha dominica andTribolium castaneum in different host grains第40-52页
    3.1. INTRODUCTION第40-41页
    3.2. MATERIAL AND METHODS第41-42页
        3.2.1 Insects第41页
        3.2.2 Rhyzopertha dominica and Tribolium castaneum rearing on different hostgrains第41-42页
        3.2.3 Rhyzopertha dominica and Tribolium castaneum adult weight and sex ratio第42页
        3.2.4 Data analysis第42页
    3.3. RESULT&DISCUSSION第42-51页
        3.3.1 Rhyzopertha dominica and Tribolium castaneum rearing on different hostgrains第42-51页
    3.4. Conclusion第51-52页
Chapter Ⅳ. Transmission electron microscopy of the antennal sensilla of Sitophilusgranarius (COLEOPTERA: CURCULIONIDAE)第52-62页
    4.1 Introduction第52-53页
    4.2. Materials and Methods第53-54页
        4.2.1. Insects第53-54页
        4.2.3. TEM第54页
    4.3. Results第54-60页
        4.3.1. TEM of Sensilla basiconica (SB)第54-55页
        4.3.2. TEM of Sensilla coeloconica (SC)第55-57页
        4.3.3. TEM of Sensilla chaetica (SCH)第57-60页
    4.4 Discussion第60-61页
    4.5 Conclusion第61-62页
Chapter Ⅴ. Transmission electron microscopy of the antennal sensilla of Triboliumcastaneum (Coleoptera: Tenebrionidae)第62-70页
    5.1. Introduction第62-63页
    5.2. MATERIALS AND METHODS第63-64页
        5.2.1. INSECT REARING第63页
        5.2.2. TRANSMISSION ELECTRON MICROSCOPY (TEM)第63-64页
        5.2.3. TERMINOLOGY AND DATA PROCESSING第64页
    5.3. RESULTS第64-68页
        5.3.1. GENERAL MORPHOLOGY OF THE ANTENNAE第64页
        5.3.2. TEM OF SENSILLA TRICHODEA第64-66页
        5.3.3. TEM of SENSILLA BASICONICA第66-68页
    5.4. Discussion第68-69页
    5.5. Conclusion第69-70页
Chapter Ⅵ. Molecular cloning of CSPs and PBPs of the lesser grain borer Rhyzoperthadominica (Coleoptera: Bostrichidae)第70-102页
    6.1 Introduction第70-71页
    6.2 Materials and Methods第71-86页
        6.2.1. Molecular cloning of CSP2s, PBP1 and PBP2 genes of Rhyzopertha dominica第71-82页
        6.2.2. Sequencing and phylogenetic analysis第82页
        6.2.3. Expression level of PBPs1 and PBPs2 of Rhyzopertha dominica第82-86页
    6.3. RESULTS AND ANALYISIS第86-102页
        6.3.1 Molecular cloning of CSP2s, PBP1 and PBP2 genes of Rhyzoperthadominica第86-90页
        6.3.2 Sequencing and phylogenetic analysis第90-98页
        6.3.3 Expression level of PBPs1 and PBPs2 of Rhyzopertha dominica第98-100页
        6.3.4 Conclusion第100-102页
References第102-111页
Acknowledgements第111-113页
Pulications list第113-114页

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