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Seinat (Cucumis melovar. tibish)的理化,营养,功能性和抗氧化特性以及其在加工食品中的应用

Acknowledgement第7-12页
List of Abbreviations第12-14页
Abstract第14-16页
摘要(Chinese abstract)第17-29页
Chapter 1 Introduction and literature review第29-60页
    1.1 Introduction第29-31页
    1.2 Significance of the research topic第31页
    1.3 Objectives of research第31-32页
    1.4 Literature review第32-52页
        1.4.1 General description of seinat(Cucumis melo var.tibish)第32-33页
        1.4.2 Seinat seeds第33-34页
        1.4.3 Cucurbits in Sudan and world第34-36页
        1.4.4 Nutritional value of cucurbit seeds第36页
        1.4.5 Cucurbit edible oilseed第36-37页
        1.4.6 Plant protein第37页
        1.4.7 Protein isolate第37-38页
        1.4.8 Amino acids of oilseeds第38页
        1.4.9 Protein hydrolyates第38页
        1.4.10 Protein fractionation第38-39页
        1.4.11 Characterization of protein第39-40页
            1.4.11.1 Electrophoresis第39页
            1.4.11.2 Thermal properties第39-40页
        1.4.12 Functional properties of oilseed protein第40-43页
            1.4.12.1 Protein solubility第40页
            1.4.12.2 Emulsifying properties第40-41页
            1.4.12.3 Water absorbance capacity第41页
            1.4.12.4 Foaming properties第41-42页
            1.4.12.5 Bulk density第42-43页
        1.4.13 Antioxidant activities of oilseed第43页
        1.4.14 Natural antioxidants第43页
        1.4.15 Tocopherol and their antioxidant activity第43-45页
        1.4.16 Antioxidant activity methods第45-48页
            1.4.16.1 DPPH radical scavenging activity第45页
            1.4.16.2 ABTS method第45-46页
            1.4.16.3 β-carotene method第46页
            1.4.16.4 Reducing power第46页
            1.4.16.5 Metal chelating activity method第46页
            1.4.16.6 Hydroxyl radical scavenging assay第46-48页
        1.4.17 Role of cucurbits oilseed in human health第48页
        1.4.18 Application of protein concentrate isolate in food systems第48-49页
        1.4.19 Fortification of bread by protein第49-51页
            1.4.19.1 Bread第49-50页
            1.4.19.2 Dough rheology第50-51页
        1.4.20 Oilseed butter第51-52页
    1.5 References第52-60页
Chapter 2 Chemical and nutritional properties of seinat (Cucumis melo var.tibish)seeds第60-75页
    2.1 Introduction第60-61页
    2.2 Materials and methods第61-64页
        2.2.1 Collection of seeds第61页
        2.2.2 Dry fruit,pulp,and seed weights第61页
        2.2.3 Proximate composition analysis of seinat seeds第61-62页
        2.2.4 Mineral analysis第62页
        2.2.5 Amino acids analysis第62页
        2.2.6 Extraction of the essential oil and its chemical composition第62-63页
        2.2.7 Scanning electron microscopy第63页
        2.2.8 Determination of vitamin content第63页
        2.2.9 Sugar profile第63-64页
    2.3 Results and discussion第64-71页
        2.3.1 Characteristics of seinat fruit and seeds第64页
        2.3.2 Proximate analysis第64-65页
        2.3.3 Mineral content第65-66页
        2.3.4 Amino acid composition第66页
        2.3.5 Chemical composition of the essential oil extracted from seinat seeds第66-70页
        2.3.6 Scanning electron microscopy第70页
        2.3.7 Vitamin content and sugar content第70-71页
    2.4 Conclusion第71-72页
    2.5 References第72-75页
Chapter 3 Physicochemical characteristics and chemical composition of seinat (Cucumis melo var. tibish)seed oil and its antioxidant activity第75-93页
    3.1 Introduction第75-76页
    3.2 Materials and methods第76-80页
        3.2.1 Chemicals and materials第76页
        3.2.2 Oil extraction第76页
        3.2.3 Color parameters第76页
        3.2.4 Oxidative stability第76页
        3.2.5 Chemical analysis of oil第76-77页
        3.2.6 Determination of fatty acid composition第77页
        3.2.7 Determination of tocopherol composition第77页
        3.2.8 Sterols analysis第77-78页
        3.2.9 Determination of total phenolics content第78页
        3.2.10 Reducing power assay第78页
        3.2.11 ABTS radical scavenging assay第78-79页
        3.2.12 DPPH radical scavenging assay第79页
        3.2.13 Antioxidant assay using the β-carotene bleaching method第79页
        3.2.14 Differential scanning calorimeter第79-80页
    3.3 Results and discussions第80-89页
        3.3.1 Chemical analysis of oil第80-81页
        3.3.2 Color第81页
        3.3.3 Oxidative stability第81-82页
        3.3.4 Fatty acid composition第82页
        3.3.5 Tocopherols content第82页
        3.3.6 Sterols第82页
        3.3.7 Total Phenolic contents of seinat seed oil第82-84页
        3.3.8 DSC thermal characteristics第84-85页
        3.3.9 Antioxidant activity of oil第85-89页
            3.3.9.1 Reducing power第85-86页
            3.3.9.2 ABTS radical assay第86-87页
            3.3.9.3 DPPH radical scavenging第87-88页
            3.3.9.4 β-carotene bleaching inhibition第88-89页
    3.4 Conclusion第89页
    3.5 References第89-93页
Chapter 4 Physiochemical and functional properties of flour and protein isolates extracted from seinat(Cucumis melo var.tibish)seeds第93-110页
    4.1 Introduction第93-94页
    4.2 Materials and methods第94-97页
        4.2.1 Materials第94页
        4.2.2 Preparation of seinat seed protein isolates第94-95页
        4.2.3 Physical analysis第95-96页
            4.2.3.1 Differential scanning calorimetric parameters第95页
            4.2.3.2 Color parameters第95页
            4.2.3.3 SDS-PAGE第95-96页
        4.2.4 Proximate composition analysis第96页
        4.2.5 Amino acids analysis第96页
        4.2.6 Mineral analysis第96-97页
        4.2.7 Determination of functional properties第97页
        4.2.8 Statistical analysis第97页
    4.3 Results and discussion第97-107页
        4.3.1 DSC thermal characteristics第97-98页
        4.3.2 Appearance of seinat fruit,seed flour,and protein isolates第98-99页
        4.3.3 Electrophoresis pattern第99-100页
        4.3.4 Proximate analysis第100-101页
        4.3.5 Mineral content第101-102页
        4.3.6 Amino acid composition第102-103页
        4.3.7 Functional properties of protein isolates第103-107页
            4.3.7.1 Protein solubility第103-104页
            4.3.7.2 Water and fat absorption capacity第104-105页
            4.3.7.3 Emulsifying capacity第105页
            4.3.7.4 Bulk density第105-106页
            4.3.7.5 Foaming capacity and foam stability第106-107页
    4.4 Conclusion第107页
    4.5 References第107-110页
Chapter 5 Influence of enzymatic hydrolysis time on the nutritional,functional and antioxidant properties ofprotein hydrolysates prepared from seinat seeds第110-130页
    5.1 Introduction第110-111页
    5.2 Materials and methods第111-115页
        5.2.1 Chemicals第111页
        5.2.2 Preparation of defatted seinat seed flour and protein isolates第111页
        5.2.3 Enzymatic hydrolysis of seinat seed protein isolate第111页
        5.2.4 Determination the degree of hydrolysis第111-112页
        5.2.5 Amino acid analysis第112页
        5.2.6 Determination of molecular weight distribution第112页
        5.2.7 Determination of nutritional characteristics第112-113页
        5.2.8 Determination of functional properties第113-114页
            5.2.8.1 Protein solubility第113页
            5.2.8.2 Water absorption capacity and oil-absorption capacity第113页
            5.2.8.3 Emulsifying activity第113-114页
            5.2.8.4 Foaming capacity and foaming stability第114页
            5.2.8.5 Emulsifying activity index and emulsion stability index第114页
            5.2.8.6 Bulk density第114页
        5.2.9 Determination of antioxidant properties第114-115页
        5.2.10 Statistical analysis第115页
    5.3 Results and discussion第115-126页
        5.3.1 Degree of hydrolysis第115-116页
        5.3.2 Amino acid composition of seinat protein hydrolysates第116-117页
        5.3.3 Molecular weight distribution第117-118页
        5.3.4 Nutritional values of seinat protein hydrolysates第118-120页
        5.3.5 Functional properties第120-122页
        5.3.6 Antioxidant activity of seinat protein hydrolysates第122-126页
    5.4 Conclusion第126页
    5.5 References第126-130页
Chapter 6 In vitro antioxidant activity of hydrolyzed protein fractions prepared from seinat seeds第130-148页
    6.1 Introduction第130-131页
    6.2 Materials and methods第131-135页
        6.2.1 Chemicals and materials第131页
        6.2.2 Preparation of defatted seinat seed flour第131-132页
        6.2.3 Fractionation of seinat seed proteins第132页
        6.2.4 Determination of protein content第132页
        6.2.5 Enzymatic hydrolysis of seinat seed protein fractions第132-133页
        6.2.6 Determination of molecular weight distribution第133页
        6.2.7 Amino acid composition第133页
        6.2.8 Antioxidant properties of seinat seed protein fractions第133-135页
            6.2.8.1 Determination of total phenolic content第133页
            6.2.8.2 Reducing power第133页
            6.2.8.3 DPPH radical scavenging activity第133-134页
            6.2.8.4 Hydroxyl radical-scavenging activity第134页
            6.2.8.5 Metal chelating activity第134-135页
        6.2.9 Statistical analysis第135页
    6.3 Results and discussion第135-144页
        6.3.1 Protein contents of seinat seed protein fractions第135-136页
        6.3.2 Molecular weight distribution第136-137页
        6.3.3 Amino acid composition第137-139页
        6.3.4 Antioxidant Activity第139-144页
            6.3.4.1 Phenolic contents of seinat seed protein fractions hydrolysate第139-140页
            6.3.4.2 Reducing power第140-141页
            6.3.4.3 DPPH radical scavenging activity第141-142页
            6.3.4.4 Hydroxyl radical scavenging activity第142-143页
            6.3.4.5 Metal chelating activity第143-144页
    6.4 Conclusion第144页
    6.5 References第144-148页
Chapter 7 Impact of seinat seed proteins addition on the sensory, nutritional and physicochemicalcharacteristics of wheat flour bread第148-166页
    7.1 Introduction第148-149页
    7.2 Materials and methods第149-152页
        7.2.1 Raw material第149页
        7.2.2 Preparation of defatted and proteins of seinat seed第149页
        7.2.3 Determination of the dough physical properties第149-151页
        7.2.4 Preparation of pan bread第151页
        7.2.5 Loaf weight and volume第151页
        7.2.6 Chemical composition第151页
        7.2.7 Mineral analysis第151-152页
        7.2.8 Amino acid analysis第152页
        7.2.9 Determination of nutritional characteristics第152页
        7.2.10 Sensory evaluation第152页
        7.2.11 Statistical analysis第152页
    7.3 Results and discussion第152-162页
        7.3.1 Chemical composition of seinat seed proteins and wheat flour第152-153页
        7.3.2 Physical properties of dough第153-155页
        7.3.3 Weight and volume of loaves第155页
        7.3.4 Sensory evaluation of bread第155-156页
        7.3.5 Proximate chemical composition of breads第156-158页
        7.3.6 Mineral content of breads第158-159页
        7.3.7 Amino acids profile of breads第159-160页
        7.3.8 Nutritional evaluation of breads第160-162页
    7.4 Conclusion第162-163页
    7.5 References第163-166页
Chapter 8 Production and evaluation of the nutritional and sensory properties of seinat butter comparable with peanut butter第166-175页
    8.1 Introduction第166-167页
    8.2 Materials and methods第167-170页
        8.2.1 Seeds preparation第167页
        8.2.2 Kernel and Hull percentage第167页
        8.2.3 Seinat seed kernel butter第167页
        8.2.4 Peanut butter第167-169页
        8.2.5 Proximate Analysis第169页
        8.2.6 Minerals content第169页
        8.2.7 In vitro protein digestibility determination第169页
        8.2.8 Sensory evaluation第169页
        8.2.9 Statistical analysis第169-170页
    8.3 Results and discussion第170-173页
        8.3.1 Kernel and hull percentage第170页
        8.3.2 Proximate composition of processed butters第170-171页
        8.3.3 Minerals content第171-172页
        8.3.4 Effect of Processing on the in vitro protein digestibility第172页
        8.3.5 Sensory evaluation第172-173页
    8.4 Conclusion第173页
    8.5 References第173-175页
Chapter 9 Oxidative stability, physicochemical characteristics and chemical composition and of seinat seedoil blends with peanut oil第175-186页
    9.1 Introduction第175-176页
    9.2 Materials and methods第176-177页
        9.2.1 Oil extraction第176页
        9.2.2 Chemical analysis of oil第176-177页
        9.2.3 Oxidative stability第177页
        9.2.4 Determination of fatty acid composition第177页
        9.2.5 Determination of tocopherol composition第177页
        9.2.6 Differential scanning calorimeter(DSC)第177页
    9.3 Results and discussion第177-183页
        9.3.1 Physiochemical analysis of oil第177-178页
        9.3.2 Oxidative stability第178-179页
        9.3.3 Fatty acid composition第179页
        9.3.4 Tocopherols content第179-181页
        9.3.5 DSC thermal characteristics第181-183页
    9.4 Conclusion第183页
    9.5 References第183-186页
General conclusions and recommendations第186-190页
    General conclusions第186-188页
    Keys innovations第188-189页
    Recommendations第189-190页
List of publications第190页

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