| Abstract | 第8-9页 |
| 1 Review of the germplasm resources, cultivation and genetic diversity of Pogostemon cablin | 第10-14页 |
| 1.1 Flower and pollen morphology | 第10页 |
| 1.2 Resource status | 第10-11页 |
| 1.3 Patchouli cultivation | 第11-12页 |
| 1.4 Genetic diversity of P.cablin | 第12页 |
| 1.5 Prospect | 第12-14页 |
| 2 Effects of consecutively monocultured Pogostemon coblin on diversity of fungal andbacterial community in rhizospheric soil | 第14-31页 |
| 2.1 Introduction | 第14-16页 |
| 2.2 Materials and methods | 第16-19页 |
| 2.2.1 Materials | 第16页 |
| 2.2.2 Methods | 第16-17页 |
| 2.2.3 Soil sampling and collection | 第17页 |
| 2.2.4 DNA extraction and purification | 第17页 |
| 2.2.5 PCR procedure and product recovery | 第17-18页 |
| 2.2.6 Restriction digestion and product isolation | 第18-19页 |
| 2.2.7 T-RFLP profile capture and statistical analysis | 第19页 |
| 2.3 Results | 第19-29页 |
| 2.3.1 Soil chemical changes | 第19-20页 |
| 2.3.2 DNA digestion and T-RFLP profile analysis of patchouli rhizosphere fungal | 第20-22页 |
| 2.3.3 DNA digestion and T-RFLP profile analysis of patchouli rhizosphere bacterial | 第22-23页 |
| 2.3.4 Analysis of the dominant fungal and bacterial species | 第23-24页 |
| 2.3.5 Effects of continuously monocultured P.cablin on Shannon-Wiener index(H) Simpson index(S) and Evenness index (E) of fungal and bacterial community in rhizosphere soil | 第24-26页 |
| 2.3.6 Effect of Soil factor on rhizosphere bacteria community structure of patchouli | 第26-27页 |
| 2.3.7 Effect of Soil factor on rhizosphere fungal community structure of patchouli | 第27-29页 |
| 2.4 Discussion and conclusions | 第29-31页 |
| 2.4.1 Effects of continuously monocultured P.cablin on the diversity of fungal community in rhizosphere soil | 第29-30页 |
| 2.4.2 Further study on continuous monoculture problems of P.cablin | 第30-31页 |
| 3 Effects of shoot root and rhizophere soil aqueous extracts of Pogostemon cablin andallelochemicals on growth and antioxidant enzymes | 第31-44页 |
| 3.1 Introduction | 第31-33页 |
| 3.2 Materials and methods | 第33-35页 |
| 3.2.1 Preparation of patchouli plants extracts | 第33页 |
| 3.2.2 Preparation of rhizosphere soil extracts | 第33页 |
| 3.2.3 Preparation of P.cablin seedlings for three autotoxicity experiments | 第33-34页 |
| 3.2.4 Autotoxicity tests with patchouli plants extracts | 第34页 |
| 3.2.5 Autotoxicity tests with rhizosphere soil extracts | 第34页 |
| 3.2.6 Autotoxicity tests with allelochemicals | 第34-35页 |
| 3.2.7 Lipid peroxidation and enzyme analyses | 第35页 |
| 3.2.8 Statistical analysis | 第35页 |
| 3.3 Results and discussion | 第35-43页 |
| 3.3.1 Autotoxicity tests with patchouli plants extracts | 第35-38页 |
| 3.3.2 Autotoxicity tests with rhizosphere soil extracts | 第38-39页 |
| 3.3.3 Autotoxicity tests with allelochemicals | 第39-42页 |
| 3.3.4 Useful measures on reducing the autotoxicity in continuous cropping of patchouli | 第42-43页 |
| 3.4 Conclusions | 第43-44页 |
| 4 Isolation and identification of autotoxic compounds from rhizosphere soil of patchouli | 第44-50页 |
| 4.1 Introduction | 第44页 |
| 4.2 Materials and methods | 第44-45页 |
| 4.2.1 Experimental designs | 第44-45页 |
| 4.2.2 Soil samples | 第45页 |
| 4.2.3 Extraction and isolation | 第45页 |
| 4.2.4 Trimethylsilylation and identification of autotoxic chemicals | 第45页 |
| 4.3 Results | 第45-48页 |
| 4.4 Discussion and conclusions | 第48-50页 |
| 5 Determination of the nutrient element of patchouli and its influence on the successioncropping obstacle | 第50-61页 |
| 5.1 Introduction | 第50-51页 |
| 5.2 Materials and methods | 第51-53页 |
| 5.2.1 Experimental Designs | 第51-52页 |
| 5.2.2 Materials | 第52页 |
| 5.2.3 Methods | 第52-53页 |
| 5.2.4 Data processing | 第53页 |
| 5.3 Results | 第53-58页 |
| 5.3.1 Changes of contents of soil organic matter,pH,macro-elements and trace elements in rhizosphere soils | 第53-55页 |
| 5.3.2 Nutrient imbalance proportion in rhizosphere soil | 第55-57页 |
| 5.3.3 Contents of macro-elements and trace elements in roots | 第57-58页 |
| 5.4 Discussion and conclusions | 第58-61页 |
| 6 Summary of the full text | 第61-63页 |
| References | 第63-68页 |
| Acknowledgements | 第68页 |
