| 摘要 | 第1-10页 |
| Abstract | 第10-13页 |
| Abbreviations | 第13-14页 |
| Chapter 1. Introduction | 第14-21页 |
| ·Somatostatin and its signaling | 第14-15页 |
| ·Somatostatin and Testis | 第15-16页 |
| ·Somatostatin and FSH in testis | 第16-17页 |
| ·Somatostatin receptor 2 on Granulosa cell | 第17-18页 |
| ·Interaction of FSH with SSTR2 in the ovary | 第18页 |
| ·Constitutive activity of SSTR2 and SSTR5 | 第18-20页 |
| ·Conclusion and hypothesis | 第20-21页 |
| Chapter 2 Somatostatin and its receptors: functional regulation in the development ofmice Sertoli cells | 第21-39页 |
| ·Material and Methods | 第21-27页 |
| ·Animals | 第21页 |
| ·Chemicals | 第21页 |
| ·Cell Isolation and Culture | 第21-23页 |
| ·RNA extraction and Real-time PCR | 第23-24页 |
| ·Immunohistochemistry(IHC)of somatostatin receptors in mice testes | 第24-25页 |
| ·Immunocytochemistry | 第25页 |
| ·cAMP measurement | 第25-26页 |
| ·Cell Cycle Analysis | 第26页 |
| ·Apoptotic activity | 第26页 |
| ·Western Blot analysis | 第26-27页 |
| ·Statistical analysis | 第27页 |
| ·Results | 第27-35页 |
| ·Expression of SSTR receptors | 第27-28页 |
| ·The expression of somatostatin receptors subtypes in different developmental stages of Sertoh cells | 第28-31页 |
| ·Forskolin-induced intracellular cAMP accumulation in SRIF14 treated-mice Sertoli cells | 第31页 |
| ·Homologous regulation of SRIF receptors | 第31-33页 |
| ·SRIF14 blocked cellular growth in mice Sertoli cells | 第33页 |
| ·SRIF increased apoptotic cells in mice Sertoli cell culture | 第33-34页 |
| ·Cell cycle and apoptotic protein expressions | 第34-35页 |
| ·SRIF14 down regulated the igfl and kitl mRNA expression in mice Sertoli cells | 第35页 |
| ·Discussion | 第35-39页 |
| Chapter 3. Constitutive activation of Somatostatin receptor 2 and 5 and their interactionwith follicle-stimulating hormone in regulating immature mouse Sertoli cells apoptosis | 第39-51页 |
| ·Material and Methods | 第39-44页 |
| ·Isolation of mice Sertoli cells (SCs) | 第39-40页 |
| ·Construction of recombinant vectors and transfection | 第40-41页 |
| ·Quantitative Polymerase chain reaction | 第41-42页 |
| ·cAMP measurements | 第42页 |
| ·Assessment of cell Apoptosis | 第42-43页 |
| ·Western blot | 第43页 |
| ·Statistical analysis | 第43-44页 |
| ·Results | 第44-48页 |
| ·Selective knock down of SSTR2 and SSTR5 in mouse SCs | 第44-45页 |
| ·Selective knockdown of SSTR2 and SSTR5 regulates ERK1/ERK2 signaling | 第45-46页 |
| ·SSTR2 and SSTR5 are important for their constitutive and FSH-induced regulation on SCs apoptosis | 第46-47页 |
| ·Effect of selective knockdown of SSTR2 and SSTR5 on basal and FSH-induced apoptotic regulatory proteins | 第47-48页 |
| ·Discussion | 第48-51页 |
| Chapter 4. Constitutive and follicle-stimulating hormone-induced action of somatostatinreceptor-2 on regulation of apoptosis and steroidogenesis in bovine granulosa cells | 第51-68页 |
| ·Material and Methods | 第51-56页 |
| ·Isolation and culture of bovine GCs | 第51-52页 |
| ·Reverse transcription-polymerase chain reaction (RT-PCR) for SSTR2 expression | 第52页 |
| ·Construction and transfection of recombinant ssRNA-1 and ssRNAi-2 vectors for SSTR2 and experimental design | 第52-53页 |
| ·Real time PCR for SSTR2 and associative genes | 第53-54页 |
| ·Measurement of estradiol and progesterone levels | 第54页 |
| ·Intracellular cAMP measurement | 第54页 |
| ·Cell apoptosis and proliferation detection | 第54-55页 |
| ·Western blot assay | 第55-56页 |
| ·Statistical analysis | 第56页 |
| ·Results | 第56-64页 |
| ·Expression of SSTR2 in bovine GCs | 第56页 |
| ·Transfection efficiency of recombinant vectors and expression in bovine GCs | 第56-58页 |
| ·Follicle-stimulating hormone(FSH)regulated the SSTR2 protein expression in bovine GCs | 第58-59页 |
| ·Treatment with FSH augmented progesterone production decreased apoptosis and increased cell proliferation in ssRNAi-2 transfected cells | 第59-62页 |
| ·SSTR2 silencing increased basal and FSH-induced progesterone production | 第62-64页 |
| ·Discussion | 第64-68页 |
| 5 Conclusion and Future Prospects | 第68-70页 |
| 6 References | 第70-79页 |
| 7. List of Figures | 第79-81页 |
| 8 List of Tables | 第81-82页 |
| 9 Acknowledgement | 第82-86页 |
| 10 Scientific Contributions | 第86-88页 |
