| 摘要 | 第3-8页 |
| abstract | 第8-10页 |
| Chapter 1 General introduction | 第14-28页 |
| 1.1 An overview of tea | 第14-16页 |
| 1.1.1 Chemical components of tea | 第14页 |
| 1.1.2 Tea polysaccharides | 第14-15页 |
| 1.1.3 Tea polyphenols | 第15-16页 |
| 1.2 Research progress on digestion and fermentation of polysaccharides | 第16-18页 |
| 1.3 Antidiabetic mechanisms related to T2DM | 第18-22页 |
| 1.3.1 α-amylase and α-glucosidase inhibitory effects | 第19页 |
| 1.3.2 Targeting β cell dysfunction | 第19-20页 |
| 1.3.3 Targeting signal pathways | 第20-21页 |
| 1.3.4 Modulation of gut microbiota | 第21-22页 |
| 1.4 Research progress on protein-polyphenols-polysaccharides interaction in drugdelivery system | 第22-25页 |
| 1.4.1 Interaction between protein and polyphenols | 第22-23页 |
| 1.4.2 Interaction between protein and polysaccharides | 第23页 |
| 1.4.3 Interaction between polyphenols and polysaccharides | 第23-24页 |
| 1.4.4 Protein-polyphenols-polysaccharides nanoparticles | 第24-25页 |
| 1.5 Significance of the study | 第25-28页 |
| Chapter 2 In vitro simulated digestion of tea polysaccharides | 第28-42页 |
| 2.1 Introduction | 第28页 |
| 2.2 General experimental procedures | 第28-29页 |
| 2.3 Methods | 第29-32页 |
| 2.3.1 TPS preparation | 第29页 |
| 2.3.2 Simulated digestion in vitro | 第29-30页 |
| 2.3.3 UV-vis spectrophotometric measurement of TPS | 第30页 |
| 2.3.4 IR fingerprint experiment of TPS | 第30-31页 |
| 2.3.5 Morphological properties of TPS | 第31页 |
| 2.3.6 Determination of molecular weight distribution of TPS | 第31页 |
| 2.3.7 Determination of reducing sugar | 第31页 |
| 2.3.8 Determination of monosaccharide changes of TPS | 第31-32页 |
| 2.3.9 α-amylase inhibitory effects of TPS | 第32页 |
| 2.3.10 Statistical analysis | 第32页 |
| 2.4 Results and Discussion | 第32-39页 |
| 2.4.1 UV-vis spectra fingerprint of TPS before and after simulated digestion in vitro | 第32-33页 |
| 2.4.2 IR spectra of TPS before and after simulated digestion in vitro | 第33-34页 |
| 2.4.3 Morphological properties of TPS before and after simulated digestion in vitro | 第34-35页 |
| 2.4.4 Molecular weight changes of TPS after simulated digestion in vitro | 第35-36页 |
| 2.4.5 Changes of the contents of reducing sugar | 第36-37页 |
| 2.4.6 Monosaccharide composition changes of TPS after gastric and intestinaldigestion | 第37-38页 |
| 2.4.7 α-amylase inhibitory effects of TPS before and after the simulated digestion | 第38-39页 |
| 2.5 Conclusion | 第39-42页 |
| Chapter 3 Interaction of EGCG with α-glucosidase and in vitro evaluation ofantidiabetic activity | 第42-56页 |
| 3.1 Introduction | 第42-43页 |
| 3.2 General experimental procedures | 第43页 |
| 3.3 Methods | 第43-47页 |
| 3.3.1 Inhibitory effects of EGCG on α-glucosidase | 第43-44页 |
| 3.3.2 Fluorescence spectroscopy analysis | 第44页 |
| 3.3.3 FI-IR spectra analysis | 第44页 |
| 3.3.4 Molecular docking analysis | 第44-45页 |
| 3.3.5 Cell culture | 第45页 |
| 3.3.6 Cell viability | 第45页 |
| 3.3.7 Glucose uptake | 第45-46页 |
| 3.3.8 Oil red o staining | 第46-47页 |
| 3.3.9 Statistical analysis | 第47页 |
| 3.4 Results and discussion | 第47-53页 |
| 3.4.1 α-Glucosidase inhibition studies | 第47-48页 |
| 3.4.2 Fluorescence quenching of α-glucosidase by EGCG | 第48-49页 |
| 3.4.3 FT-IR measurements | 第49-50页 |
| 3.4.4 Molecular docking analysis | 第50-51页 |
| 3.4.5 Glucose uptake | 第51-52页 |
| 3.4.6 Oil red o staining | 第52-53页 |
| 3.5 Conclusion | 第53-56页 |
| Chapter 4 Encapsulation of EGCG in BSA/Tea Polysaccharides nanoparticles | 第56-68页 |
| 4.1 Introduction | 第56-57页 |
| 4.2 General experimental procedures | 第57页 |
| 4.3 Methods | 第57-60页 |
| 4.3.1 TPS-2 (30-80 KDa) preparation | 第57页 |
| 4.3.2 Nanoparticle preparation | 第57-58页 |
| 4.3.3 Characterization of nanoparticles | 第58-59页 |
| 4.3.4 In vitro release of EGCG from nanoparticles | 第59页 |
| 4.3.5 Antioxidant activities | 第59-60页 |
| 4.3.6 Statistical analysis | 第60页 |
| 4.4 Results and discussion | 第60-66页 |
| 4.4.1 Loading efficiency and loading capacity | 第60-61页 |
| 4.4.2 Characterization of nanoparticles | 第61-64页 |
| 4.4.3 In vitro release of EGCG from nanoparticles | 第64页 |
| 4.4.4 Antioxidant activities | 第64-66页 |
| 4.5 Conclusion | 第66-68页 |
| Chapter 5 Conclusions and prospect | 第68-70页 |
| References | 第70-82页 |
| Appendix | 第82-84页 |
| Publications and participation in scientific research | 第84-86页 |
| Acknowledgement | 第86页 |
