| Abstract | 第6-7页 |
| Abbreviations | 第8-9页 |
| 1 Literature Review | 第9-23页 |
| 1.1 Introduction | 第9-10页 |
| 1.2 Nitrate assimilation | 第10-13页 |
| 1.3 Ammonium assimilation | 第13-14页 |
| 1.4 Genes involved in ammonium assimilation | 第14-16页 |
| 1.5 Nitrogen assimilating enzymes | 第16-19页 |
| 1.6 Information about gene families involved in N transporters and assimilation | 第19-21页 |
| 1.7 Literature reviews about PEX 11 gene | 第21-23页 |
| 2 Objectives and Significance of the Proposed Research | 第23-25页 |
| 2.1 Objectives | 第23页 |
| 2.2 Significance | 第23-25页 |
| 3 Materials and Methods | 第25-30页 |
| 3.1 Constructs and Transformation | 第25-26页 |
| 3.2 Northern blot and Southern blot Analysis | 第26页 |
| 3.3 Plant growth conditions | 第26页 |
| 3.4 Identification of PEX11 homologs in rice | 第26-27页 |
| 3.5 Protein sequence analysis of the PEX11 genes in different species | 第27页 |
| 3.6 Phylogenetic analysis of the PEX11 family genes | 第27-28页 |
| 3.7 Promoter sequence analysis of the OsPEX11 family genes | 第28页 |
| 3.8 Ka/Ks analysis to determine the similarity between OsPEX11-2 and OsPEX11-3 | 第28页 |
| 3.9 Expression profile analysis of PEX11 family genes in rice | 第28-29页 |
| 3.10 Differential expression of OsPEX11 gene family to different kinds of stresses | 第29-30页 |
| 4 Results | 第30-59页 |
| 4.1 Selecting the candidate genes | 第30-32页 |
| 4.2 Overexpression of the candidate genes | 第32-35页 |
| 4.3 Silencing the candidate genes and promoter analysis | 第35-38页 |
| 4.4 Special behavior of one of the candidate gene(LOC_Os07g05820) | 第38-39页 |
| 4.5 Results of PEX11 gene family;Identification of PEX11 homologs in rice | 第39-43页 |
| 4.6 Protein sequence analysis of the PEX11 family members | 第43-46页 |
| 4.7 Phylogenctic analysis of PEX11 family members in all the related species | 第46-48页 |
| 4.8 Promoter sequence analysis of PEX11 gene family members in rice and Arabidopsis | 第48-53页 |
| 4.9 Divergence analysis | 第53-54页 |
| 4.10 Expression profile analysis of PEX11 family in rice | 第54-56页 |
| 4.11 Ka/Ks analysis for OsPEX11 gene family members | 第56-57页 |
| 4.12 Differential responses of PEX11 gene family members to different kinds of stresses | 第57-59页 |
| 5 Discussions | 第59-67页 |
| 5.1 Possible reasons for the failure of the first study | 第59-60页 |
| 5.2 Evolution of PEX11 gene family | 第60-62页 |
| 5.3 Expression profile analysis of PEX11 family genes in rice | 第62-63页 |
| 5.4 Differential expression of PEX11 genes to different kinds of abiotic stresses | 第63-66页 |
| 5.5 Brief perspective of future study | 第66-67页 |
| References | 第67-76页 |
| Protocol | 第76-89页 |
| Resume | 第89-90页 |
| Acknowledgement | 第90-91页 |
