| List of Abbreviations | 第12-13页 |
| Chinese abstract | 第13-16页 |
| Abstract | 第16-18页 |
| Chapter 1: General Introduction | 第19-35页 |
| 1.1 Background of okra crop | 第19-20页 |
| 1.2 Plant tissue culture | 第20-21页 |
| 1.3 Okra tissue culture | 第21页 |
| 1.4 Phenolic compounds secretion from explant and their lethal effects on plant tissueculture | 第21-22页 |
| 1.5 Oxidative browning in plant tissue culture | 第22-24页 |
| 1.6 Factors affecting phenolic secretion | 第24页 |
| 1.7 Strategies to overcome phenolic secretion and culture browning | 第24-25页 |
| 1.8 Mechanism of browning control by additives in culture medium | 第25-26页 |
| 1.9 Limitations of anti-browning additives in plant tissue culture | 第26-28页 |
| 1.10 Plant cell and tissue culture an attractive alternative for secondary metabolitesproduction | 第28-32页 |
| 1.10.1 In vitro anthocyanin production | 第28-29页 |
| 1.10.2 Factors affecting in vitro anthocyanin production | 第29页 |
| 1.10.3 Nitrogen deficiency as a way to modulate anthocyanin accumulation in invitro cultures | 第29-32页 |
| 1.10.4 Light intensity effects anthocyanin accumulation in plant tissues | 第32页 |
| 1.11 Project aims and objectives | 第32-35页 |
| Chapter 2: In vitro regeneration of Abelmoschus esculentus L. cv. Wufu: Influence ofanti-browning additives on phenolic secretion and callus formation frequency inexplants | 第35-57页 |
| 2.1 Introduction | 第37-38页 |
| 2.2 Materials and methods | 第38-42页 |
| 2.2.1 Seed material | 第38-39页 |
| 2.2.2 Media fortification with anti-browning additives | 第39页 |
| 2.2.3 Callus induction | 第39页 |
| 2.2.4 Callus growth and development | 第39-41页 |
| 2.2.5 Shoot induction and elongation | 第41页 |
| 2.2.6 Root formation and acclimatization of plantlets | 第41页 |
| 2.2.7 Statistical analysis | 第41-42页 |
| 2.3 Results | 第42-52页 |
| 2.3.1 Influence of anti-browning additives on phenolic secretion in callus culture | 第42-45页 |
| 2.3.2 Influence of plant growth regulators on callus induction | 第45页 |
| 2.3.3 Interaction effect of anti-browning additives and PGRs on callus induction | 第45-49页 |
| 2.3.4 Callus growth and development | 第49页 |
| 2.3.5 Shoot regeneration | 第49页 |
| 2.3.6 Rooting and acclimatization of plantlets | 第49-52页 |
| 2.4 Discussion | 第52-55页 |
| 2.5 Conclusion | 第55-57页 |
| Chapter 3: Ascorbic acid controls lethal browning and Pluronic F-68 promotes high-frequency multiple shoot regeneration from cotyldonary node explant of okra(Abelmoschusesculentus.L) | 第57-75页 |
| 3.1 Introduction | 第59-61页 |
| 3.2 Materials and methods | 第61-64页 |
| 3.2.1 Preparation of ascorbic acid, silver nitrate, and Pluronic F-68 stock solutions | 第61页 |
| 3.2.2 Surface sterilization and seed germination | 第61-62页 |
| 3.2.3 Fortification of anti-browning additives in culture medium | 第62页 |
| 3.2.4 Culturing of explants on anti-browning additives supplemented medium | 第62页 |
| 3.2.5 Multiple shoots induction and proliferation | 第62-63页 |
| 3.2.6 Shoot elongation | 第63页 |
| 3.2.7 In vitro rooting and acclimatization of plantlets | 第63页 |
| 3.2.8 Hardening and field transfer | 第63页 |
| 3.2.9 Statistical analysis | 第63-64页 |
| 3.3 Results | 第64-71页 |
| 3.3.1 Culture establishment | 第64-65页 |
| 3.3.2 Effects of anti-browning additives on phenolic secretion and culture vigor | 第65-66页 |
| 3.3.3 Effects of plant growth regulators and regeneration enhancers on shoot initiation andmultiplication | 第66页 |
| 3.3.4 Effects of plant growth regulators and regeneration enhancers on phenotypes ofregenerated shoots | 第66-68页 |
| 3.3.5 Shoot elongation | 第68-69页 |
| 3.3.6 In vitro rooting and acclimatization | 第69-71页 |
| 3.4 Discussion | 第71-74页 |
| 3.5 Conclusion | 第74-75页 |
| Chapter 4: Accumulation of anthocyanin in callus cultures of red-pod okra[Abelmoschus esculentus (L.) Hongjiao] in response to light and nitrogen levels | 第75-95页 |
| 4.1 Introduction | 第77-79页 |
| 4.2 Materials and methods | 第79-83页 |
| 4.2.1 Surface sterilization and seed germination | 第79页 |
| 4.2.2 Explant preparation and callus induction | 第79页 |
| 4.2.3 Light irradiation and media composition for anthocyanin induction | 第79-81页 |
| 4.2.4 Determination of callus biomass | 第81页 |
| 4.2.5 Determination of total anthocyanin (TA) content | 第81页 |
| 4.2.6 Determination of total phenolic (TP) content | 第81-82页 |
| 4.2.7 Determination of total antioxidant activity (TAA) | 第82页 |
| 4.2.8 Total flavonoid (TF) content | 第82页 |
| 4.2.9 Microscopic analysis | 第82页 |
| 4.2.10 HPLC analysis | 第82-83页 |
| 4.2.11 Statistical analysis | 第83页 |
| 4.3 Results and discussion | 第83-94页 |
| 4.3.1 Callus culture | 第83-84页 |
| 4.3.2 Effects of nitrogen level and light intensity on callus biomass accumulation | 第84-85页 |
| 4.3.3 Effects of different nitrogen concentrations and light irradiation on anthocyanininduction | 第85-88页 |
| 4.3.4 Callus biomass accumulation and its correlation with anthocyanin accumulation | 第88-89页 |
| 4.3.5 Phenolic content,flavonid accmulation,and antioxidant activity in callus culturesunder different culture conditions | 第89-92页 |
| 4.3.6 Pigment composition in callus cultures | 第92-94页 |
| 4.4 Conclusion | 第94-95页 |
| Chapter 5: General discussion and conclusions | 第95-99页 |
| References | 第99-117页 |
| Appendices | 第117-159页 |
| Publications | 第159-161页 |
| Acknowledgements | 第161页 |
