| 摘要 | 第1-9页 |
| Abstract | 第9-14页 |
| DEDICATION | 第14-34页 |
| Abbreviation | 第34-36页 |
| CHAPTER-1 INTRODUCTION | 第36-54页 |
| ·BACKGROUND OF COTTON | 第36-39页 |
| ·Utilization | 第36页 |
| ·Cultivation and distribution | 第36-37页 |
| ·Current status | 第37-39页 |
| ·MAJOR DISEASES | 第39-40页 |
| ·VERTICILLIUM WILT | 第40-50页 |
| ·Cause of Verticillium wilt | 第40-41页 |
| ·Symptoms | 第41页 |
| ·Perpetuation | 第41-43页 |
| ·Host Plants | 第43页 |
| ·Lifecycle and disease process | 第43页 |
| ·Yield losses | 第43-44页 |
| ·Isolation, identification, and molecular characterization | 第44-45页 |
| ·Management of Verticillium wilt | 第45-50页 |
| ·Resistance germplasm | 第46-47页 |
| ·Chemical control | 第47-48页 |
| ·Organic amendments | 第48页 |
| ·Biological control | 第48-49页 |
| ·Physical control | 第49-50页 |
| ·Molecular studies | 第50页 |
| ·HHPOTHESIS,SALIENT FEATURES AND OBJECTIVES OF RESEARCH | 第50-54页 |
| ·Hypothesis | 第50-52页 |
| ·Salient features | 第52-53页 |
| ·Objectives | 第53-54页 |
| CHAPTER-2 FUNGAL DIVERSITY ASSOCIATED WITH VERTICILLIUM WILT | 第54-102页 |
| ·SUMMARY | 第54-56页 |
| ·INTRODUCTION | 第56-58页 |
| ·MATERIALS AND METHODS | 第58-65页 |
| ·Collection of samples | 第58页 |
| ·Isolation of fungi | 第58-61页 |
| ·Soil assay | 第58-61页 |
| ·Soil dilution plate | 第58页 |
| ·Soil dry plate | 第58-59页 |
| ·Baiting | 第59页 |
| ·Bioassay | 第59-61页 |
| ·COTTON PLANT ASSAY | 第61页 |
| ·Identification of the fungi | 第61-63页 |
| ·Morphological examination | 第61页 |
| ·Molecular identification | 第61-63页 |
| ·DNA extraction | 第61-62页 |
| ·Polymerase chain reaction (PCR) | 第62-63页 |
| ·Sequencing | 第63页 |
| ·IN VITRO EFFICACY OF ANTAGONISTIC FUNGI | 第63页 |
| ·SOME PHYSIOLOGICAL STUDIES OF VERTICILLIUM DAHLIAE | 第63-64页 |
| ·Effect of different media | 第63-64页 |
| ·Effect of temperatures | 第64页 |
| ·Effect of light | 第64页 |
| ·Effect of Hydrogen ion concentration | 第64页 |
| ·STATISTICAL ANALYSIS | 第64-65页 |
| ·RESULTS | 第65-71页 |
| ·Identification of fungal diversity | 第65页 |
| ·Fungi associated with Verticillium wilt infested soil | 第65页 |
| ·Fungi associated with Verticillium wilt of cotton | 第65页 |
| ·Comparison of fungal species associated with soil and cotton plant | 第65-66页 |
| ·Frequency of fungi | 第66-68页 |
| ·Soil plating | 第66页 |
| ·Soil dilution | 第66-67页 |
| ·Baiting and bioassay | 第67-68页 |
| ·Direct isolation from cotton plants | 第68页 |
| ·In vitro efficacy of antagonistic fungi | 第68-69页 |
| ·Effect of some physiological factors on Verticillium dahliae | 第69-71页 |
| ·Effect of different culture media | 第69页 |
| ·Effect of various temperatures | 第69页 |
| ·Effect of light regimes | 第69页 |
| ·Effect of Hydrogen ion concentrations | 第69-71页 |
| ·DISCUSSION | 第71-76页 |
| ·Association of fungi with Verticillium wilt of cotton | 第71-72页 |
| ·Frequency of fungi | 第72-74页 |
| ·Effect of some physiological factors on the development of V. dahliae | 第74-76页 |
| 附录 | 第76-102页 |
| CHAPTER-3 SCREENING FOR HIGH RESISTANCE AND ELITE COTTON GERMPLASMTHROUGH IN VIVO AND IN VITRO EXPERIMENTS AGAINST VERTICILLIUM DAHLIAE KLEB. | 第102-148页 |
| ·SUMMARY | 第102-103页 |
| ·INTRODUCTION | 第103-105页 |
| ·MATERIALS AND METHODS | 第105-111页 |
| ·In vivo experiments | 第105-107页 |
| ·In vivo disease assessment | 第105-107页 |
| ·Wilt incidence | 第105-106页 |
| ·Foliar discoloration | 第106页 |
| ·Vascular discoloration | 第106页 |
| ·Mortality and live plant percentage | 第106-107页 |
| ·Infection rate or intensity of infection (%) of V. dahliae | 第107页 |
| ·Identification of V. dahliae | 第107页 |
| ·In vitro experiments | 第107-109页 |
| ·Inoculation methods | 第108页 |
| ·Inoculum preparation | 第108-109页 |
| ·In vitro disease assessment | 第109页 |
| ·Re-isolation of the fungus | 第109页 |
| ·Resistance and susceptibility | 第109-110页 |
| ·Yield and fibre quality | 第110页 |
| ·Statistical analysis | 第110-111页 |
| ·RESULTS | 第111-118页 |
| ·In vivo disease assessment | 第111-112页 |
| ·Identification of Verticillium species | 第111-112页 |
| ·In vitro disease assessment | 第112-114页 |
| ·Seed germination | 第112页 |
| ·Wlpercentages | 第112页 |
| ·Infection rate (%) of V. dahliae | 第112-113页 |
| ·Foliar discoloration | 第113页 |
| ·Mortality and live plant percentage | 第113页 |
| ·Effect on plant growth | 第113-114页 |
| ·Effect of different inoculation methods | 第114-116页 |
| ·Comparison of in vivo and in vitro screening methods | 第116页 |
| ·Resistance and susceptibility ranking | 第116页 |
| ·Fibre quality and yield | 第116-117页 |
| ·Correlation of some meteorological factors | 第117-118页 |
| ·DISCUSSION | 第118-121页 |
| ·Rapid screening of cotton germplasm with high resistance to V. dahliae | 第118-119页 |
| ·Effect of different inoculation methods | 第119-120页 |
| ·Influence of Verticillium wilt on yield and fibre quality | 第120-121页 |
| ·Influence of meteorological factors on Verticillium wilt | 第121页 |
| ·CONCLUSION | 第121-123页 |
| 附录 | 第123-148页 |
| CHAPTER 4 CHEMICAL INDUCER FOR THE RESISTANCE OF COTTON GERMPLASMAGAINST VERTICILLIUM WILT | 第148-202页 |
| ·SUMMARY | 第148-149页 |
| ·INTRODUCTION | 第149-151页 |
| ·MATERIALS AND METHODS | 第151-155页 |
| ·The elite use of butylated hydroxytoluene (BHT) and sodium alginate (SA) along with conventional fungicides for the suppression of Verticillium wilt of cotton | 第151-153页 |
| ·Fungicides and chemical compound formulation | 第151页 |
| ·In vitro efficacy test of BHT and SA combinations with fungicides | 第151-152页 |
| ·Effcacy of BHT and SA combinations with fungicides under greenhouse | 第152-153页 |
| ·Seed treatment and application methods | 第152页 |
| ·Assessment of treatments efficacy | 第152-153页 |
| ·The elite uses of some selective chemical elicitor for the suppression of Verticillium wilt of cotton | 第153-154页 |
| ·Chemical compound | 第153页 |
| ·Preliminary antifungal test of chemical compounds | 第153页 |
| ·Integrative potential test of chemical compounds under greenhouse | 第153-154页 |
| ·Seed treatment and chemical compound application | 第154页 |
| ·Assessment of treatments efficacy | 第154页 |
| ·Statistical analysis | 第154-155页 |
| ·RESULTS | 第155-162页 |
| ·The elite use of butylated hydroxytoluene (BHT) and sodium alginate (SA) along with conventional fungicides for the suppression of Verticillium wilt of cotton | 第155-158页 |
| ·In vitro efficacy of BHT and SA combinations against the V. dahliae | 第155页 |
| ·Efficacy of BHT and SA combinations with Carbendazim and Topsin-Magainst Ⅴ. dahliae under greenhouse | 第155-158页 |
| ·Wilt incidence (WI) | 第155-156页 |
| ·Infection percentage | 第156页 |
| ·Seed germination | 第156-157页 |
| ·Plant growth promotion | 第157页 |
| ·Response of different treatment methods | 第157-158页 |
| ·The elite uses of some selective chemical elicitor for the suppression of Verticillium wilt of cotton | 第158-162页 |
| ·In vitro antifungal effect of chemical compounds against the V. dahliae | 第158页 |
| ·Integrative potential of pure chemical compounds under greenhouse | 第158-162页 |
| ·Antifungal activity | 第158-160页 |
| ·Plant growth promotions | 第160-162页 |
| ·DISCUSSIONS | 第162-166页 |
| ·Role of BHT and SA combinations with the conventional fungicides | 第162-164页 |
| ·Antifungal potential of pure chemical compounds against the V. dahliae | 第164-165页 |
| ·Plant growth promotion | 第165-166页 |
| ·CONCLUSIONS | 第166-167页 |
| 附录 | 第167-202页 |
| CHAPTER-5 EFFICIENT USE OF SOME POTENTIAL BIOLOGICAL CONTROL AGENTSAGAINSTVERTICILLIUM WILT OF COTTON | 第202-258页 |
| ·SUMMARY | 第202-203页 |
| ·INTRODUCTION | 第203-206页 |
| ·MATERIALS AND METHODS | 第206-215页 |
| ·Plant Materials | 第206页 |
| ·Pathogen culture | 第206页 |
| ·Source of potential biological control agents (BCAs) | 第206页 |
| ·In vitro and greenhouse potential test of fungal BCAs | 第206-209页 |
| ·In vitro potential test of BCAs | 第206-208页 |
| ·Dual culture assay | 第206-207页 |
| ·BCAs alone | 第206-207页 |
| ·BCAs with safe chemica | 第207页 |
| ·Volatile metabolites assay | 第207-208页 |
| ·Greenhouse potential test of fungal BCAs | 第208-209页 |
| ·Application of potential fungal BCAs | 第208-209页 |
| ·Seed treatment (ST) assay | 第208-209页 |
| ·Spore drench (SD) assay | 第209页 |
| ·Solid culture mix (SCM) assay | 第209页 |
| ·In vitro and greenhouse potential test of bacterial BCAs | 第209-211页 |
| ·In vitro potential test of bacterial BCAs | 第209-211页 |
| ·Dual culture assay | 第209-210页 |
| ·BCAs alone | 第209-210页 |
| ·BCAs with safe chemical | 第210页 |
| ·Volatile metabolites assay | 第210-211页 |
| ·Greenhouse potential test of bacterial BCAs | 第211页 |
| ·Application of potential BCAs | 第211页 |
| ·Seed treatment (ST) assay | 第211页 |
| ·Spore drench (SD) assay | 第211页 |
| ·Inoculums preparation | 第211-213页 |
| ·Pathogen inoculums | 第211-212页 |
| ·Antagonistic (BCAs) inoculums | 第212-213页 |
| ·Seed coating | 第213页 |
| ·Seed coating with fungal BCAs | 第213页 |
| ·Seed coating with bacterial BCAs | 第213页 |
| ·Assessment of greenhouse treatments efficacy | 第213-214页 |
| ·Statistical analysis | 第214-215页 |
| ·RESULTS | 第215-221页 |
| ·Efficay of fungal BCAs under in vitro and greenhouse conditions | 第215-217页 |
| ·In vitro effect of fungal BCAs on mycelial colony growth of Ⅴ. dahliae | 第215-216页 |
| ·Efficacy of four potential fungal BCA under greenhouse conditions | 第216-217页 |
| ·Influence on WI, efficacy, DS, DSI, mortality and live (%) | 第216-217页 |
| ·Influence of treatment methods | 第217页 |
| ·Promotion of Plant growth | 第217页 |
| ·Efficay of bacterial BCAs under in vitro and greenhouse conditions | 第217-221页 |
| ·In vitro effect of bacterial BCAs on mycelial colony growth of V. dahliae | 第217-218页 |
| ·Efficacy of four potential bacterial BCA under greenhouse conditions | 第218-221页 |
| ·Influence on WI, DS, DSI, mortality and live (%) | 第218-219页 |
| ·Promotion of Plant growth | 第219-220页 |
| ·Influence of treatment methods | 第220-221页 |
| ·DISCUSSION | 第221-227页 |
| ·Antagonistic potential of BCAs | 第222-226页 |
| ·Antagonistic potential of fungal BCAs | 第222-224页 |
| ·Antagonistic potential of bacterial BCAs | 第224-225页 |
| ·Incorporation of safe chemicals | 第225-226页 |
| ·Efficiency of BCAs applications | 第226-227页 |
| ·Conclusion | 第227-228页 |
| 附录 | 第228-258页 |
| CHAPTER-6 CHINESE MEDICINAL PLANTS:AN ALTERNATIVE APPROACH FORMANAGEMENT OF VERTICILLIUM WILT OF COTTON | 第258-280页 |
| ·SUMMARY | 第258-259页 |
| ·INTRODUCTION | 第259-260页 |
| ·MATERIALS AND METHODS | 第260-263页 |
| ·Plant Materials | 第260页 |
| ·Source of Chinese medicinal herbs | 第260页 |
| ·Pathogen inoculums | 第260页 |
| ·Preparation of stock solution | 第260-261页 |
| ·Preliminary in vitro antifungal test | 第261页 |
| ·Integrative potential test of four selective Chinese herbs under greenhouse | 第261-263页 |
| ·Seed treatment and application of Chinese herbs | 第262页 |
| ·Assessment of treatments efficacy | 第262-263页 |
| ·Statistical analysis | 第263页 |
| ·Results | 第263-266页 |
| ·Antifungal potential of Chinese herbs against the V. dahliae | 第263页 |
| ·Integrative potential of chemical compounds under greenhouse | 第263-266页 |
| ·Antifungal activity | 第263-264页 |
| ·Plant growth promotions | 第264-265页 |
| ·Effect of different treatment methods of application | 第265-266页 |
| ·DISCUSSIONS | 第266-269页 |
| ·Antifungal potential of Chinese herbs against the V. dahliae | 第266-267页 |
| ·Plant growth promotion | 第267-269页 |
| 附录 | 第269-280页 |
| CHAPTER-7 ANALYSIS OF TRANSCRIPTOME FOR DIFFERENTIALLY EXPRESSED GENES(DEGS) INVOLVED IN MECHANISM OF VERTICILLIUM WILT RESISTANT OF COTTON | 第280-326页 |
| ·SUMMARY | 第280-282页 |
| ·INTRODUCTION | 第282-285页 |
| ·MATERIALS AND METHODS | 第285-291页 |
| ·Plant material | 第285页 |
| ·Inoculation of plants | 第285页 |
| ·Sample collection | 第285页 |
| ·RNA extraction | 第285-287页 |
| ·RNA-seq library construction and sequencing | 第287页 |
| ·Alignment of RNA-seq quality control | 第287页 |
| ·De novo assembly | 第287-288页 |
| ·Annotation and classification of all Unigenes | 第288-289页 |
| ·Expression of Transcript abundance | 第289页 |
| ·Assessment of differentially expressed genes (DEGs) | 第289-290页 |
| ·GO and KEGG enrichment analysis | 第290页 |
| ·Identification of differentially expressed genes following Ⅴ. dahliae infection | 第290-291页 |
| ·RESULTS | 第291-296页 |
| ·Reads quality control | 第291页 |
| ·De novo assembly | 第291页 |
| ·Annotation and classification all Unigenes | 第291-292页 |
| ·Expression of Transcript abundance | 第292-293页 |
| ·Assessment of differentially expressed genes (DEGs) | 第293页 |
| ·Enrichment analysis of GO and KEGG | 第293页 |
| ·Identification of DEGs following Ⅴ. dahliae infection | 第293-294页 |
| ·Functional classification of DEGs responsive to defense mechanism of cotton plant against Ⅴ. dahliae infection | 第294-295页 |
| ·Identification of highly expressed genes responsive to defense mechanism of cotton plant against Ⅴ. dahliae infection | 第295-296页 |
| ·DISCUSSION | 第296-300页 |
| ·Transcriptome analysis of Arcot-1 and Arcot-438 germplasm | 第296-297页 |
| ·DEGs following Ⅴ. dahliae infection | 第297-300页 |
| 附录 | 第300-326页 |
| CHAPTER-8 GENERAL CONCLUSION | 第326-331页 |
| SUGGESTIONS | 第331-332页 |
| REFERENCES | 第332-360页 |
| APPENDICES | 第360-398页 |
| ACKNOWLEDGMENT | 第398-400页 |
| AUTHORS' BIOGRAPHY | 第400-404页 |
